A5012181098- Immunotherapy and Immune Responses
- RNA Interference and Gene Delivery
- Nanofabrication and Lithography Techniques
- Polymer Surface Interaction Studies
- Immune Response and Inflammation
- Rice Cultivation and Yield Improvement
- Advanced Drug Delivery Systems
- Advanced Biosensing Techniques and Applications
- Inhalation and Respiratory Drug Delivery
- Force Microscopy Techniques and Applications
- Plant responses to water stress
- Microfluidic and Capillary Electrophoresis Applications
- Climate change impacts on agriculture
- 3D Printing in Biomedical Research
- Blood properties and coagulation
- Aerosol Filtration and Electrostatic Precipitation
- Hepatitis B Virus Studies
- Innovative Microfluidic and Catalytic Techniques Innovation
- Hydrogels: synthesis, properties, applications
- GABA and Rice Research
- Electrowetting and Microfluidic Technologies
- Microfluidic and Bio-sensing Technologies
- Antimicrobial Peptides and Activities
- Liquid Crystal Research Advancements
- Molecular Junctions and Nanostructures
Dalian University
2017-2025
Dalian University of Technology
2017-2025
Jiangsu Institute of Meteorological Sciences
2023
China Meteorological Administration
2023
University of California, Los Angeles
2012-2017
California NanoSystems Institute
2013-2017
University of Illinois Urbana-Champaign
2011-2012
National University of Singapore
2007-2011
China Agricultural University
2008-2009
Chinese Academy of Meteorological Sciences
2005
The protein resistance of poly(N-isopropylacrylamide) brushes grafted from silicon wafers was investigated as a function the chain molecular weight, grafting density, and temperature. Above lower critical solution temperature (LCST) 32 °C, collapse water-swollen chains, determined by ellipsometry, depends on density weight. Ellipsometry, radio assay, fluorescence imaging demonstrated that, below temperature, repel effectively oligoethylene oxide-terminated monolayers. very low levels adsorb...
The penetration behavior of nanoparticles in mucous depends on physicochemical properties the and mucus microenvironment, due to particle–mucin interactions presence mucin mesh space filtration effect. To date, it is still unclear how surface influence their behaviors various physiological pathophysiological conditions. In this study, we have prepared a comprehensive library amine-, carboxyl-, PEG-modified silica (SNPs) with controlled ligand densities. Using multiple particle tracking,...
Abstract Here, a novel method of immobilizing proteins with well‐defined orientation directly on liquid crystal surfaces that allow subsequent real‐time imaging specific protein–protein binding events these is reported. Self‐assembly nitrilotriacetic acid terminated amphiphiles loaded Ni 2+ ions at aqueous‐liquid interface creates surface capable histidine‐tagged ubiquitin through complex formation between and histidine. When containing immobilized are exposed to anti‐ubiquitin antibody, the...
Abstract Protein adsorption and reversible cell attachment are investigated as a function of the grafting density poly( N‐ isopropyl acrylamide) (PNIPAM) brushes. Prior studies demonstrated that thermally driven collapse grafted PNIPAM above lower critical solution temperature 32 °C is not required for protein adsorption. Here, dependence reversible, protein‐mediated adhesion on polymer chain density, below temperature, reported. Above °C, brushes from non‐adsorbing, oligo(ethylene...
Virus-like particles (VLPs) are self-assembled viral proteins that represent a superior form of antigens in vaccine formulations. To enhance immunogenicity, adjuvants, especially the aluminum salts (Alum), essentially formulated VLP vaccines. However, Alum only induce biased humoral immune responses limits further applications VLP-based stimulate more balanced immunity, we, herein, develop one-step strategy using VLPs as biotemplates to synthesize raspberry-like silica-adjuvanted VLP@Silica...
Zwitterionic polymers have emerged as promising trans-mucus nanocarriers due to their superior antifouling properties. However, for pH-sensitive zwitterionic polymers, the effect of pH microenvironment on fate remains unclear. In this work, we prepared a library polydopamine-modified silica nanoparticles (SiNPs-PDA) with an isoelectric point 5.6. Multiple-particle tracking showed that diffusion SiNPs-PDA in mucus value 5.6 was 3 times faster than 3.0 or 7.0. Biophysical analysis found...
Abstract Monosodium urate (MSU) crystal deposition in joints can lead to the infiltration of neutrophils and macrophages, their activation plays a critical role pathological progress gout. However, MSU physicochemical properties inducing cell death neutrophil macrophage is still unclear. In this study, crystals different sizes are synthesized explore pyroptosis It demonstrated that induce size‐dependent pyroptotic bone marrow‐derived (BMNs) macrophages (BMDMs) by triggering NLRP3...
Protein assays are critical analytical tools performed in various biochemical laboratories to quantify the concentration of proteins. In this study, we report optical responses a thin layer liquid crystals supported on glass slides decorated with proteins and utility phenomenon as new "all-or-nothing" type protein assay. It was found that orientations very sensitive solution applied surface. When exceeds value (IgG 5.0 μg/mL, BSA 6.0 FTIC-anti-biotin 0.40 FITC-anti-IgG 0.37 μg/mL), gives...
Aluminum-salt-based vaccine adjuvants are prevailingly used in FDA-approved vaccines for the prevention of infectious diseases over eighty years. Despite their safe applications, mechanisms regarding how material characteristics affect interactions at nano–bio interface and immunogenicity remain unclear. Recently, studies have indicated that activation NLRP3 inflammasome plays a critical role inducing adjuvant effects controlled by inherent shape hydroxyl contents aluminum oxyhydroxide...
With the wide application of Stöber silica nanoparticles and their ability to access brain, it is crucial evaluate neurotoxicity. In this study, we used three in vitro model cells, i.e., N9, bEnd.3 HT22 representing microglia, microendothelial cells neurons, respectively, assess neurotoxicity with different sizes. We found that almost had no effect on viability cells. contrast, they induced size-dependent toxicity N9 which represent residential macrophages central nervous system. Further...
This study investigated the impact of protein adsorption mechanism(s) on efficiency thermally controlled cell adhesion and release from poly(N-isopropyl acrylamide) brushes. Large format polymer gradients were used to screen for grafting densities substrate chemistries that alter both at 37 °C rapid 25 °C. In particular, conditions allowed underlying substrate, penetration brush only, or outer edge film. At an average molecular weight 30 kDa (degree polymerization N ∼ 270), results show...
The orientational transition of liquid crystals (LCs) is used as a label-free detection mechanism for immunoassays developed in microfluidic systems. LCs only show bright optical textures (visible to the naked eye) line-line intersections which antibodies bind their surface-immobilized antigens, suggesting feasibility using detect specific antigen-antibody binding events high-throughput and multiplexed manner.
Physicochemical properties of nanomaterials play important roles in determining their toxicological profiles during nano-biointeraction. Among them, surface modification is one the most effective manners to tune cytotoxicity induced by nanomaterials. However, currently, there no consistency including moiety types and quantities considering conflicting particles across different studies. In this study, order systematically investigate how density affects NPs, we chose three functional groups,...
Key factors governing the suspension stabilities of aluminum-based adjuvants: particle size, surface charge, free energy and hydrophobicity.
In this paper, we report the role of surfactants in minimizing nonspecific protein adsorption liquid crystal (LC)-based immunoassays which LC is used as a readout system. Among all tested, only nonionic surfactant such Tween 20 can effectively reduce adsorption, while maintaining selectivity LC-based immunoassay. We also show that to minimize be added directly into antibody solution final concentration 0.8 mM. After addition 20, better correlations between concentrations and interference...
The interaction between the aluminum salt-based adjuvants and antigen in vaccine formulation is one of determining factors affecting immuno-potentiation effect vaccines. However, it not clear how intrinsic properties could affect this interaction, which limits to benefit improvement existing further new Here, we engineered oxyhydroxide (AlOOH) nanorods used a variety antigens including hepatitis B surface (HBsAg), SARS-CoV-2 spike protein receptor-binding domain (RBD), bovine serum albumin...
Complex substrate control is demonstrated with a dual-tone hydrogel photoresist. By exposing photodegradable to UV light through photomask, both swollen and eroded micropatterns decreased modulus can be created on the surface under different exposure conditions. This provides an important tool for investigating synergistic effects of spatially heterogeneous mechanical topological cues cell behavior.