A5073887845- Bacteriophages and microbial interactions
- Genomics and Phylogenetic Studies
- Antibiotic Resistance in Bacteria
- Microbial infections and disease research
- Vibrio bacteria research studies
- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- Bacterial Genetics and Biotechnology
- Plant Pathogenic Bacteria Studies
- Plant Virus Research Studies
- Bacterial biofilms and quorum sensing
- Antibiotic Use and Resistance
- Legume Nitrogen Fixing Symbiosis
- CRISPR and Genetic Engineering
- Evolution and Genetic Dynamics
- Salmonella and Campylobacter epidemiology
- Respiratory viral infections research
- RNA modifications and cancer
- Antimicrobial Peptides and Activities
- Ocular Surface and Contact Lens
- Biochemical and Structural Characterization
- MicroRNA in disease regulation
- Antifungal resistance and susceptibility
- Protein Structure and Dynamics
- T-cell and B-cell Immunology
Fraunhofer Institute for Cell Therapy and Immunology
2021-2025
Zhejiang University-University of Edinburgh Institute
2018-2022
Sir Run Run Shaw Hospital
2021-2022
Zhejiang University
2021-2022
Xi’an Jiaotong-Liverpool University
2021
Singapore General Hospital
2017-2018
University of Hohenheim
2016-2017
Max Planck Society
2014
Max Planck Institute for Developmental Biology
2013-2014
Medical Research Council
2010
Nonsense-mediated mRNA decay (NMD) is a eukaryotic quality control mechanism that detects aberrant mRNAs containing nonsense codons and induces their rapid degradation. This degradation mediated by SMG6, an NMD-specific endonuclease, as well the SMG5 SMG7 proteins, which recruit general enzymes. However, it remains unknown specific factors are recruited whether this recruitment direct. Here, we show binds directly to POP2, catalytic subunit of CCR4–NOT deadenylase complex, elicits...
Abstract Phage-antibiotic combinations to treat bacterial infections are gaining increased attention due the synergistic effects often observed when applying both components together. Most studies however focus on a single pathogen, although in many clinical cases multiple species present at site of infection. The aim this study was investigate anti-biofilm activity phage-antibiotic/antifungal single- and dual-species biofilms formed by P. aeruginosa fungal pathogen Candida albicans ....
The removal of the 5′-cap structure by decapping enzyme DCP2 and its coactivator DCP1 shuts down translation exposes mRNA to 5′-to-3′ exonucleolytic degradation XRN1. Although yeast directly interact, an additional factor, EDC4, promotes DCP1–DCP2 association in metazoan. Here, we elucidate how human proteins interact assemble active complex decapped mRNAs are handed over We show that EDC4 serves as a scaffold for assembly, providing binding sites DCP1, XRN1 bind simultaneously C-terminal...
Multidrug-resistant bacterial infections are becoming increasingly common, with only few last-resort antibiotics such as colistin available for clinical therapy. An alternative therapeutic strategy gaining momentum is phage therapy, which has the advantage of not being affected by resistance to antibiotics. However, a major challenge in therapy rapid emergence phage-resistant bacteria. In this work, our main aim was understand mechanisms phage-resistance used top priority pathogen...
Non-antibiotic alternative treatments to combat the increasing number of infections caused by multidrug resistant bacteria are urgently needed. In recent years, bacteriophages have reemerged potentially replace or complement role antibiotics, as bacterial viruses ability inactivate pathogens. This study aimed evaluate synergy phage–antibiotic combinations. A Citrobacter amalonaticus isolate was used in this together with phage MRM57. Eight different antibiotics mechanisms action were...
The CCR4-NOT complex plays a crucial role in post-transcriptional mRNA regulation eukaryotes. This catalyzes the removal of poly(A) tails, thereby repressing translation and committing an to degradation. conserved core is assembled by interaction at least two modules: NOT module, which minimally consists NOT1, NOT2 NOT3, catalytic module comprising deadenylases, CCR4 POP2/CAF1. Additional subunits include CAF40 newly identified human subunits, NOT10 C2orf29. C2orf29 how they are integrated...
Targeting host factors is a complementary strategy for the development of new antiviral drugs. We screened library isoxazolidine and isoxazole sulfonamides found four compounds that inhibited HIV‐1 infection in human CD4+ lymphocytic T cells with no toxicity at IC 90 concentrations. Structure‐activity relationship showed benzyl halo‐substituted aromatic ring on heterocycle scaffold were critical antiretroviral activity. The size position incorporated halogen had marked effect sulfonamide...
Acinetobacter baumannii is of major clinical importance as the bacterial pathogen often causes hospital acquired infections, further complicated by high prevalence antibiotic resistant strains. Aside from natural tolerance to certain classes, resistance exchange genetic information via conjugation but also competence exhibited A. . In addition, bacteriophages are able introduce genes toxins and virulence factors phage mediated transduction. this work, we analyzed complete genomes 177 strains...
Chemical genetics is an emerging approach to investigate the biology of host-pathogen interactions. We screened several inhibitors ATP-dependent DNA motors and detected gyrase B inhibitor coumermycin A1 (C-A1) as a potent antiretroviral. C-A1 inhibited HIV-1 integration gene expression from acutely infected cell, but two activities mapped distinct targets. Target discovery identified Hsp90 target affecting viral expression. Chromatin immunoprecipitation revealed that associates with promoter...
Phage therapy represents a possible treatment option to cure infections caused by multidrug-resistant bacteria, including methicillin and vancomycin-resistant Staphylococcus aureus , which most antibiotics have become ineffective. In the present study, we report isolation complete characterization of novel phage named JD219 exhibiting broad host range able infect 61 138 clinical strains S. tested, included MRSA as well. The JD419 exhibits unique morphology with an elongated capsid flexible...
Infections caused by Pseudomonas aeruginosa are becoming increasingly difficult to treat due the emergence of strains that have acquired multidrug resistance. Therefore, phage therapy has gained attention as an alternative treatment pseudomonal infections. Phages not only bactericidal but occasionally show activity against biofilm well. In this study, we describe Motto, a T1-like can clear P. infections in animal model and also exhibits biofilm-degrading properties. The substantial...
Bacteriophages and bacteriophage-derived peptidoglycan hydrolases (endolysins) present promising alternatives for the treatment of infections caused by multidrug resistant Gram-negative Gram-positive pathogens. In this study, Gp105, a putative lysozyme murein hydrolase from Enterobacter phage myPSH1140 was characterized in silico, vitro as well vivo using purified protein. Gp105 contains T4-type lysozyme-like domain (IPR001165) belongs to Glycoside family 24 (IPR002196). The endolysin indeed...
Abstract Introduction: While neoadjuvant chemotherapy with interval debulking surgery (IDS) has comparable clinical outcomes to primary (PDS) for advanced epithelial ovarian cancer, their economic dimension remains understudied. Methods: This retrospective chart review examined Stage IIIC–IV cancer patients who underwent IDS or PDS between 2011 and 2014. We compared the demographics, disease-specific, intraoperative, thirty-day outcome billing, ten-year survival data. Results: Patients ( n =...
Phages utilize lysis systems to allow the release of newly assembled viral particles that kill bacterial host. This is also case for phage AP1, which infects rice pathogen Acidovorax oryzae. However, how occurs on a molecular level currently unknown. We performed in silico bioinformatics analyses, indicated cassette contains holin (HolAP) and endolysin (LysAP), are encoded by two adjacent genes. Recombinant expression LysAP caused Escherichia coli lysis, while HolAP arrested growth....
Our trust in modern medicine is currently eroding as we come to realize the global health crisis that caused by pathogens are resistant many, if not all, antibiotics. Soon, will be exposed a situation similar era before discovery of Penicillin, where an ordinary injury, simple cut -if infected- could result our lives coming abrupt end.1 With emergence bacteria antibiotic [antimicrobial resistance (AMR)], governments many countries have recognized criticality this issue and started programmes...