Introduction: The emergence of antibiotic resistance is a significant challenge in the treatment bacterial infections, particularly patients intensive care unit (ICU). Phage-antibiotic combination therapy now being utilized as preferred therapeutic option for infections that are multi-drug resistant nature. Methods: In this study, we examined combined impact staph phage vB_Sau_S90 and four antibiotics on methicillin-resistant Staphylococcus aureus (MRSA). We conducted experiments three...

Bacteriophages and bacteriophage-derived peptidoglycan hydrolases (endolysins) present promising alternatives for the treatment of infections caused by multidrug resistant Gram-negative Gram-positive pathogens. In this study, Gp105, a putative lysozyme murein hydrolase from Enterobacter phage myPSH1140 was characterized in silico, vitro as well vivo using purified protein. Gp105 contains T4-type lysozyme-like domain (IPR001165) belongs to Glycoside family 24 (IPR002196). The endolysin indeed...

Treatment failure remains an issue in periprosthetic joint infection (PJI). Bacteriophages offer new treatment options. However, there is still a lack of evidence to better define their usefulness and administration. We report case which antibiotic suppression was successful only after administration bacteriophages. Antibiotic the option for 94-year-old male with methicillin-resistant Staphylococcus aureus (MRSA) PJI hip knee. As could not be suppressed adequately, bacteriophages were...

The emergence of multi-drug resistance in Salmonella , causing food-borne infections, is a significant issue. With over 2,600 serovars sp., it crucial to identify specific solutions for each serovar. Phage therapy serves as an alternate treatment option. In this study, vB_SalP_792 phage was obtained from sewage, forming plaques eight out 13 tested clinical S. enterica isolates. Transmission electron microscopy (TEM) examination revealed T7-like morphotype. characterized by its stability,...

Validating the efficacy and safety of phages prior to clinical application is crucial see phage therapy in practice. Current animal models include mice insect larvae, which pose ethical or technical challenges.

(Background) Numerous reports on carbapenem resistance in different parts of India have been published, yet there are insufficient studies the prevalence antibiotic northeast region country. This study evaluated emergence clinical isolates collected Mizoram. (Results) A total 141 Gram-negative were from two hospitals, including Civil Hospital Lunglei and Synod Aizawl. The include Escherichia coli (n = 62, 43.9%), Klebsiella spp. 43, 30.4%), Pseudomonas aeruginosa 9, 6.3%), Serratia...

The influence of media composition on the life cycle bacteriophages to exhibit diverse plaque morphology various bacteriological was investigated by a double agar overlay method. Both Staphylococcus aureus phage and Vibrio parahaemolyticus showed altered from small large clear turbid, in different culture used for

Abstract Increasing antibiotic resistance poses a serious threat, especially in patients admitted to ICUs. The use of phages combination with antibiotics as compassionate therapy has become choice treatment for pan-drug-resistant bacteria. Here, we studied the cumulative effect four antibiotics, fosfomycin, ciprofloxacin, vancomycin and oxacillin using three different orders against S. aureus . disc synergy method showed that plaque size phage increased subinhibitory zone. sub-inhibitory...

A BSTRACT The current survey was conducted to assess the knowledge, awareness and perception of platelet-rich plasma (PRP) on accelerated tooth movement among dentist present in state Tamil Nadu. Nadu dentists were subjects cross-sectional questionnaire survey. 500 participants completed self-administered questionnaires about their awareness, PRP’s effect movement. Statistical Package for Social Sciences (SPSS) (V 22.0) used do statistical analysis. It computed frequency distribution.The...

Abstract Background Staphylococcus aureus is one of the common opportunistic gram-positive pathogens which are often associated with nosocomial infections. Detection methicillin-resistant S. (MRSA) has become complicated due to complex phenotypic and genomic pattern. Objective To evaluate sensitivity specificity pattern various methods used in screening mec genes harboring MRSA. Methods Clinical isolates were collected from diagnostic centers Tamil Nadu. Phenotypic identification such as...

Staphylococcus aureus is a pathogen endemic in India and sometimes deadly for patients intensive care units.To determine the antibiotic-resistance pattern, biofilm forming ability, clonal type of S. from isolates collected Tamil Nadu (south) Mizoram (northeast) regions India.We diagnostic laboratories Mizoram. An antibiotic susceptibility test was performed according to Clinical Laboratory Standards Institute methods. Antibiotic-resistant determinants such as mecA, mecC, blaZ, vanA, vanB,...

We describe the complete genome sequence of bacteriophage Motto, which infects clinical strains Pseudomonas aeruginosa. Motto is a T1-like siphovirus related to members family Drexlerviridae and has capsid width ~57 nm tail length ~255 nm. The 49.9-kb contains 84 protein-coding genes.

Abstract Methicillin-Resistant Staphylococcus aureus is a human pathogen. MRSA has acquired resistance to major antibiotics; thus, phage therapy become potential alternative treatment. In this work, two broad host range phages were characterized for lifecycle, physio-chemical parameters and bacterial killing kinetics, the in vitro behavior of insensitive cells serial passage multiple doses assessed by reduction turbidity spot assay. Phage vB_Sau_S90 showed an absorption efficiency 91 ± 0.6%...

Abstract In order to establish phage therapy as a standard clinical treatment for bacterial infections, testing of every ensure the suitability and safety biological compound is required. While some issues have been addressed over recent years, easy-to-use animal models test phages are still rare. Testing in highly suitable mammalian such mice subjected strict ethical regulations, while insect larvae Galleria mellonella model suffers from batch-to-batch variations requires manual operator...

Abstract Bacteriophages and bacteriophage-derived peptidoglycan hydrolases (endolysins) present promising alternatives for the treatment of infections caused by multi-drug resistant Gram-negative Gram-positive pathogens. In this study, Gp105, a putative lysozyme murein hydrolase from Enterobacter phage myPSH1140 was characterized in silico, vitro as well vivo using purified protein. Gp105 contains T4-type lysozyme-like domain (IPR001165) belongs to Glycoside family 24 (IPR002196). The...