A5102949051- Evolution and Genetic Dynamics
- Bacterial Identification and Susceptibility Testing
- Nosocomial Infections in ICU
- Innovative Microfluidic and Catalytic Techniques Innovation
- vaccines and immunoinformatics approaches
- Temporomandibular Joint Disorders
- Cancer Treatment and Pharmacology
- Neutropenia and Cancer Infections
- Antimicrobial Peptides and Activities
- Bacterial Genetics and Biotechnology
- Antibiotic Resistance in Bacteria
- Gene Regulatory Network Analysis
- Biochemical and Structural Characterization
- Hemispheric Asymmetry in Neuroscience
- Electrowetting and Microfluidic Technologies
- Voice and Speech Disorders
- 3D Printing in Biomedical Research
- Nanoparticle-Based Drug Delivery
- Cystic Fibrosis Research Advances
- Brain Metastases and Treatment
- Estrogen and related hormone effects
- Parkinson's Disease and Spinal Disorders
- Facial Nerve Paralysis Treatment and Research
- Urinary Tract Infections Management
- Phytoestrogen effects and research
Centre National de la Recherche Scientifique
2021-2022
Institut Pasteur
2021-2022
Université Paris Cité
2022
Shionogi (Japan)
2019
University of Glasgow
2015-2018
University of Fukui
2016
University of Fukui Hospital
2016
Osaka University
1982-2014
Yamamoto Hospital
2006
Tokyo University of Science
2002
Multi-drug strategies have been attempted to prolong the efficacy of existing antibiotics, but with limited success. Here we show that evolution multi-drug-resistant Escherichia coli can be manipulated in vitro by administering pairs antibiotics and switching between them ON/OFF manner. Using a multiplexed cell culture system, find certain combinations completely suppresses development resistance one antibiotics. this data, develop simple deterministic model, which allows us predict fate...
Three dimensional (3D) printing is actively sought after in recent years as a promising novel technology to construct complex objects, which scope spans from nano- over millimeter scale. Previously we utilized Fused deposition modeling (FDM)-based 3D printer chemical fluidic systems, and here demonstrate the construction of milli-fluidic structures for programmable liquid handling control biological samples. Basic operation devices, such water-in-oil (W/O) droplet generators producing...
Bacterial interactions with surfaces rely on the coordinated expression of a vast repertoire surface-exposed adhesins. However, how bacteria dynamically modulate their adhesion potential to achieve successful surface colonization is not yet well understood. Here, we investigated changes in capacity an initially poorly adherent Escherichia coli strain using experimental evolution and positive selection for mutations improving biofilm formation abiotic surfaces. We showed that all identified...
In bacteria, cell size affects chromosome replication, the assembly of division machinery, wall synthesis, membrane synthesis and ultimately growth rate. addition, can also be a target for Darwinian evolution protection from predators. This strong coupling growth, however, could lead to introduction defects after evolution. An important question remains: bacterial change and/or evolve without imposing burden?The directed particular sizes, burden, was tested with laboratory Escherichia coli...
ObjectiveThe epidemiology and role of the anti-PcrV titer in non-cystic fibrosis patients with Pseudomonas aeruginosa airway tract infections is not fully understood. This study was performed to compare titers without P. respiratory infections.MethodsThis prospective cohort conducted at Hokkaido University Hospital Japan. Participants had blood sputum specimens collected on admission. They were divided into two groups based their culture results. Those a infection assigned (PA) group those...
The number of effective antibiotics is rapidly decreasing due to the emergence resistant bacteria, which necessitates development not only new, but novel strategies for developing antibiotics. Antimicrobial peptides (AMPs) are considered be a promising new class antibiotics, and predict potential candidates still actively sought after. Here, we present closed-loop artificial intelligence-based approach that combines an in silico genetic algorithm, machine learning prediction, vitro...
We clinically investigated a total of 288 cases bacteremia for the past ten years, from January 1986 to December 1995, at Second Department Internal Medicine in jikei University Hospital.All subjects who had positive reaction blood culture or catheter tip were their basic disease, complications, and detected bacteria. Malignant tumors, chronic renal failure, diabetes mellitus, hematologic disease frequent by noted.The due primary infection mainly respiratory organ urinary tract infection,...
Chronic alcohol consumption affects brain functions such as learning, memory and motor skills, which constitute signal transduction, neural transmission, ion channels. However, any roles of ethanol in expression channels the have never been clarified. In this study, we evaluated effects on mRNA rat slices. The experiment with organotypic slice cultures was performed to examine various genes expression. Wistar male rats (8 weeks old) were anesthetized by carbon dioxide immediately...
It is well known that acute EtOH consumption results in poor prognosis after traumatic brain injury (TBI). Survival rate was dropped about 50% by edema augmentation TBI under rat. Water channel aquaporin-4 (AQP4) related to edema. We previously reported AQP4 important role swelling EtOH. And affects sodium ion concentration. be regulated Na-K ATPase, Na(+)-K(+)-2Cl(-) co-transporter. involved those channels. These findings indicate may affect expression In this study, rat primary astrocyte...
ABSTRACT Bacterial interactions with surfaces rely on the coordinated expression and interplay of surface exposed adhesion factors. However, how bacteria dynamically modulate their vast repertoire adhesins to achieve colonization is not yet well-understood. We used experimental evolution positive selection for improved investigate an initially poorly adherent Escherichia coli strain increased its capacities abiotic surfaces. showed that all identified evolved clones acquired mutations...