A5024853354- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Autoimmune and Inflammatory Disorders Research
- Birth, Development, and Health
- IL-33, ST2, and ILC Pathways
- Single-cell and spatial transcriptomics
- Renal and related cancers
- Hematopoietic Stem Cell Transplantation
- Pancreatic function and diabetes
- Fish biology, ecology, and behavior
- Immune cells in cancer
- Congenital heart defects research
- Assisted Reproductive Technology and Twin Pregnancy
- Pregnancy and preeclampsia studies
- Immunotherapy and Immune Responses
- Cancer Genomics and Diagnostics
- Fish Ecology and Management Studies
Cincinnati Children's Hospital Medical Center
2021-2024
Children's Hospital & Medical Center
2022-2024
University of Cincinnati
2021
Abstract Analysis of the human hematopoietic progenitor compartment is being transformed by single-cell multimodal approaches. Cellular indexing transcriptomes and epitopes sequencing (CITE-seq) enables coupled surface protein transcriptome profiling, thereby revealing genomic programs underlying states. To perform CITE-seq systematically on primary bone marrow cells, we used titrations with 266 antibodies (antibody-derived tags) machine learning to optimize a panel 132 antibodies....
The dynamics of the hematopoietic flux responsible for blood cell production in native conditions remains a matter debate. Using CITE-seq analyses, we uncovered distinct progenitor population that displays cycle gene signature similar to one found quiescent stem cells. We further determined CD62L marker can be used phenotypically enrich this Flt3+ multipotent (MPP4) compartment. Functional vitro and vivo analyses validated heterogeneity MPP4 compartment established quiescent/slow-cycling...
Macrophage activation syndrome (MAS) is a life-threatening cytokine storm complicating systemic juvenile idiopathic arthritis (SJIA) driven by IFN-γ. SJIA and MAS are also associated with an unexplained emerging inflammatory lung disease (SJIA-LD), our recent work supporting pulmonary of IFN-γ pathways pathologically linking SJIA-LD MAS. Our objective was to mechanistically define the potentially novel observation inflammation in TLR9 mouse model In acute MAS, lungs exhibit mild but diffuse...
Most nephrons are added in late gestation. Truncated extrauterine nephrogenesis premature infants results fewer and significantly increased risk for CKD adulthood. To overcome the ethical technical difficulties associated with studies of late-gestation human fetal kidney development, third-trimester rhesus macaques served as a model to understand lateral branch (LBN) at molecular level.
Systemic autoimmune and autoinflammatory diseases are characterized by genetic cellular heterogeneity. While current single-cell genomics methods provide insights into known disease subtypes, these analysis do not readily reveal novel cell-type perturbation programs shared among distinct patient subsets. Here, we performed RNA-Seq of PBMCs patients with systemic juvenile idiopathic arthritis (SJIA) diverse clinical manifestations, including macrophage activation syndrome (MAS) lung (LD). We...
Abstract How two-chambered hearts in basal vertebrates have evolved from single-chamber found ancestral chordates remains unclear. Here, we show that the teleost sinus venosus (SV) is a chamber-like vessel comprised of an outer layer smooth muscle cells. We find adult zebrafish nr2f1a mutants, which lack atria, SV comes to physically resemble thicker bulbus arteriosus (BA) at arterial pole heart through adaptive, hypertensive response involving proliferation due aberrant hemodynamic flow....
Abstract Plasma cell (PC) precursors are generated during a germinal center (GC) response and migrate to the bone marrow (BM) where they undergo terminal differentiation into short-lived (SL) or long-lived (LL) antibody secreting cells. The developmental dynamics genomic states of PC remain be elucidated1. We utilize novel experimental system, involving CD138+ splenocytes isolated from NP-KLH immunized C57Bl/6J mice (21–42 dpi) their adoptive transfer B deficient mMT mice, analyze...