A5014670331- Epigenetics and DNA Methylation
- Cancer-related gene regulation
- Mass Spectrometry Techniques and Applications
- Bioactive Compounds and Antitumor Agents
- Metabolomics and Mass Spectrometry Studies
- Cancer Genomics and Diagnostics
- Genomics and Chromatin Dynamics
- Histone Deacetylase Inhibitors Research
- Signaling Pathways in Disease
- RNA Research and Splicing
- Cancer therapeutics and mechanisms
- Advanced Proteomics Techniques and Applications
Broad Institute
2022-2025
Eli and Edythe Broad Foundation
2024
Abstract KMT2C and KMT2D , encoding histone H3 lysine 4 methyltransferases, are among the most commonly mutated genes in triple-negative breast cancer (TNBC). However, how these mutations may shape epigenomic transcriptomic landscapes to promote tumorigenesis is largely unknown. Here we describe that deletion of Kmt2c or Kmt2d non-metastatic murine models TNBC drives metastasis, especially brain. Global chromatin profiling immunoprecipitation followed by sequencing revealed altered H3K4me1,...
Despite the widespread use of MS for hydrogen/deuterium exchange measurements, no systematic, large-scale study has been conducted to compare observed rates in protein-derived, unstructured peptides measured by predicted calculated from NMR-derived values and how neighboring residues post-translational modifications influence those rates. In this study, we sought test accuracy performing hydrogen measurements on whole cell digests generate an unbiased dataset 563 unique derived...