An efficient and high yielding expression system is required to produce recombinant proteins. Furthermore, the transient can be used identify localization of proteins in plant cells. In this study, we demonstrated that combination a geminiviral replication double terminator dramatically enhanced protein level plants. The GFP was expressed transiently lettuce, Nicotiana benthamiana, tomatoes, eggplants, hot peppers, melons, orchids with agroinfiltration. Compared single terminator, level. A...

Cells elongate on a surface with nanogrooved (NG) patterns and align along that pattern. Although various models have been proposed for how this occurs, much remains to be clarified. Studies fixed cells do not lend themselves answering some of these open questions. In study, the dynamic behaviours living mesenchymal stem an NG substrate 200 nm groove depth, 870 ridge width 670 were observed using time-lapse microscopes. We found filopodia moved as if they probing surroundings cell...

Collagen hydrogel is a popular extracellular matrix (ECM) material in regenerative medicine and has an isotropic structure. In contrast, native ECM anisotropic Electrospinning of collagen dissolved organic solvents widely used for fabricating nanofibres; however, such fibres are water-soluble require cross-linking before use as scaffolds cell culture. Herein, electrospinning using core-shell nozzle was employed to spin aqueous acidic solution encapsulate it within shell polyvinylpyrrolidone...

Abstract Background The oncological outcome of patients who underwent curative surgery for lower rectal cancer was investigated to clarify whether lateral pelvic lymph node dissection (LPLD) conferred any benefit. Methods A total 246 stage II and III (below the peritoneal reflection) between 1985 1998 reviewed. Forty-two these did not undergo LPLD. Results Patients LPLD were older, more likely have anterior resection nerve preservation, had smaller tumours metastasis at an earlier than those...

Cell behavior can be manipulated by the topography of culture surface. In this study, we examined intercellular communication and osteogenic differentiation mesenchymal stem cells (MSCs) grown on electrospun fibers with different orientations densities. Human bone marrow-derived MSCs (hMSCs) were seeded poly(ε-caprolactone) (PCL) scaffolds composed aligned (1D) or cross-aligned (2D) (1.0–1.2 µm diameter) high, medium, low fiber It was found that preferred to adhere onto PCL rather than flat...

Hydrogel nanofibers derived from alginate with an anisotropic structure fabricated by using core–shell electrospinning play important role in cell adhesion and proliferation as the extracellular matrix (ECM).

An investigation into the acute toxicity of mechanically fibrillated cellulose nanofibers (fib-CNFs), with a fiber length ranging from 500 to 600 nm, was conducted in accordance OECD TG412 guidelines. In this study, rats were exposed fib-CNFs via nasal inhalation for 6 h daily over 28-day period. The highest exposure concentration set at 35 mg/m3, intermediate and low concentrations 7.0 mg/m3 1.5 respectively. No significant differences observed body weight, hematological parameters, or...

In this study, we aimed to develop soy protein-derived edible porous hydrogel scaffolds for cultured meat based on mechanical anisotropy mimic the physical and biochemical properties of muscle tissues. Based traditional Japanese Ito-Kanten (thread agar) freeze–thaw process, used liquid nitrogen directional freezing combined with ion crosslinking fabricate an aligned scaffold composed protein isolate (SPI), carrageenan (CA), sodium alginate (SA). SPI, CA, SA were dissolved in water, heated,...

The prognosis for patients with liver metastases from colorectal cancer is still poor. Thus, patient selection hepatic resection essential to improve the poor results of procedure. Some reports have shown that synchronous worse than those metachronous metastases. Therefore, determination factors influence outcome after more important metastasis.We studied who had been followed 5 years undergoing cancer.Among 12 prognostic (age, gender, adjuvant chemotherapy, tumor site, CEA level,...

A novel optical FSK heterodyne single filter detection system, which can use direct modulation and tolerate the relatively large spectral width of a laser diode, is proposed. The receiver sensitivity improvement, as much 8 dB over using Ge APD, has been achieved after 105 km transmission at 100 Mbit/s.

Here we show a new strategy for efficient freeze concentration-mediated cytoplasmic delivery of proteins, obtained via the endosomal escape property polyampholyte-modified liposomes. The concentration method successfully induces internalization proteins simply by freezing cells with protein and nanocarrier complexes. However, mechanism remains unclear. Here, designed novel carrier modifying liposomes through incorporating hydrophobic polyampholytes therein. These complexes were characterized...

Electrospun nanofibers, featured a lower glass-transition temperature than the freezing and loose mesh structure, allows direct cryopreservation of adherent cells towards investigation cell-material composites.

The development of sustained control drug release for delivering hydrophilic drugs has been challenging due to a burst release. Nanofibers are used as materials that enable efficient delivery systems. In this study, we designed drug-encapsulated core-shell nanofibers comprising core collagen (Col) incorporated with berberine chloride (BC), an anti-inflammatory and anti-cancer agent model drug, hydrophobic shell poly-l-lactic acid (PLLA). Long-term profiles under both the physiological...

This study was undertaken to evaluate the long-term prognostic significance of conventional peritoneal cytology in patients with advanced colorectal carcinoma after curative resection.A review performed 189 who underwent resection for pT3/T4 colon and upper/middle rectum between March 1987 December 1991. Patient outcomes were reviewed retrospectively. Peritoneal before manipulation tumor. Intraoperatively, 50 ml saline instilled 20 reaspirated cytology. In all patients, Papanicolaou Giemsa...

To investigate the outcome of surgical treatment for patients with T4 rectal cancer and to evaluate prognostic factors influencing 5-year disease-free survival.Of 1600 cancers seen between 1985 1998, there were 197 whom 128 treated curative intent. In this retrospective study organ invaded, type analysed.Of patients, 89% had visceral involvement 11% pelvic wall involvement. The most frequently involved was bladder, followed by prostate vagina. Low anterior resection performed in 52,...

Ten secondary metabolites were identified from Goniothalamus tapis Miq. The structures of these compounds elucidated using spectroscopic analyses. It was revealed for the first time that 3-methyl-1H-benz[f]indole-4,9-dione produced by organism.

In 1991, the Japan Research Society for Cancer of Colon and Rectum proposed clinical criteria (Japanese criteria) hereditary nonpolyposis colorectal cancer (HNPCC). According to Japanese Amsterdam criteria, three groups patients are defined: 1) those diagnosed as having HNPCC using (Amsterdam HNPCQ; 2) classified category A (putative HNPCC-A) 3) B HNPCC-B). order evaluate prognosis role clinicopathological characteristics, recurrence survival rates (n 14) putative HNPCC-A 31) 100) were...

To investigate whether cystic dilation may be due to a reduction of postcholinergic cell bodies that leads autonomic dysfunction, ganglion were counted in five patients with choledochal cysts and normal choledochus. In the choledochus, counts proximal distal portions 374.7 +/- 82.7/cm2 349.2 51.2/cm2, respectively. cases cysts, dilated portion narrow 298.6 86.9/cm2 81.0 34.8/cm2, The ratio (narrow dilated) was 0.271. Significant reductions numbers observed all having various types...

Nanofibrous nonwoven fabrics have attracted attention as porous adsorbents with high specific surface areas for the safe and efficient treatment of spilled organic dyes petroleum. For this purpose, a method fabricating nanofibers would be highly beneficial. In study, phase separation in electrospun from blended solutions immiscible polymers [poly(styrene) (PS) poly(vinylpyrrolidone) (PVP)] was investigated. The removal PVP sacrificial polymer afforded imprinting mesopores (40-70 nm) PS...