A5018862316- Genomics and Chromatin Dynamics
- Coal Combustion and Slurry Processing
- Advanced Power Generation Technologies
- RNA Research and Splicing
- Coal and Coke Industries Research
- Industrial Engineering and Technologies
- Electrospun Nanofibers in Biomedical Applications
- HIV Research and Treatment
- Bacteriophages and microbial interactions
- DNA Repair Mechanisms
- Chromosomal and Genetic Variations
- RNA Interference and Gene Delivery
- RNA and protein synthesis mechanisms
- Engineering Technology and Methodologies
- Periodontal Regeneration and Treatments
- Molecular Biology Techniques and Applications
- Tissue Engineering and Regenerative Medicine
- Bone Tissue Engineering Materials
- HIV/AIDS drug development and treatment
- Engineering Diagnostics and Reliability
- CRISPR and Genetic Engineering
- Thermochemical Biomass Conversion Processes
- Radiation Therapy and Dosimetry
- Cell death mechanisms and regulation
- Nuclear Issues and Defense
Federal Medical-Biological Agency
2022-2025
All-Russia Thermal Engineering Institute
2009-2024
Obninsk Institute for Nuclear Power Engineering
2020
Institute for Physics and Power Engineering
2020
National Research Nuclear University MEPhI
2019
Engelhardt Institute of Molecular Biology
2006-2017
Russian Academy of Sciences
2006-2013
Insufficient vascular growth in the area of artificial-material implantation contributes to ischemia, fibrosis, development bacterial infections, and tissue necrosis around graft. The purpose this study was evaluate angiogenesis after polycaprolactone microfiber scaffolds modified by a pCMV-VEGF165-plasmid rats. Influence vascularization on scaffold degradation also examined. We investigated flat microfibrous obtained electrospinning with incorporation pCMV-VEGF-165 plasmid into microfibers...
DNA double-strand breaks (DSBs) are involved in many cellular mechanisms, including replication, transcription, and genome rearrangements. The recent observation that hot spots of DSBs human chromosomes delimit domains possess coordinately expressed genes suggests a strong relationship between the organization transcription patterns DSBs. In this study, we performed mapping 43-kb ribosomal (rDNA) repeated unit. We observed rDNA units corresponded to most fragile sites these possessed at...
Immune responses to tissue-engineered grafts made of xenogeneic materials remain poorly studied. The scope current investigations is limited by the lack information on orthotopically implanted grafts. A deeper understanding these processes great importance since innovative surgical approaches include implantation decellularized scaffolds seeded cells. purpose our work study immunological features tracheal repair during constructs based human modified via laser radiation in rabbits. samples...
Genome instability plays a key role in multiple biological processes and diseases, including cancer. Genome-wide mapping of DNA double-strand breaks (DSBs) is important for understanding both chromosomal architecture specific regions at DSBs. We developed method precise genome-wide blunt-ended DSBs human chromosomes, observed non-random fragmentation DSB hot spots. These spots are scattered along chromosomes delimit protected 50–250 kb domains. found that about 30% the domains (denoted forum...
Abstract Endogenous hot spots of DNA double-strand breaks (DSBs) are tightly linked with transcription patterns and cancer genomics 1,2 . There nine DSBs located in human rDNA units 3–6 Here we describe that the profiles these coincide γ-H2AX or H2AX, strongly suggesting a high level vivo breakage inside genes. The data were confirmed by microscopic observation largest foci nucleoli interphase chromosomes. In metaphase chromosomes, observed only some portion clusters possess all co-localize...
The extracellular matrix is an organized three-dimensional network of protein-based molecules and other macromolecules that provide structural biochemical support to tissues. Depending on its properties, the influences cell adhesion signal transduction and, in general, can influence differentiation proliferation through specific mechanisms chemical mechanical sensing. development body tissues during ontogenesis accompanied by changes not only cells but also composition properties matrix....
Uncovering the nature of communication between enhancers, promoters and insulators is important for understanding fundamental mechanisms that ensure appropriate gene expression levels. Here we describe an approach employing transient genetic luciferase reporter constructs with quantitative RT–PCR analysis transcription enhancer Hsp70 promoter. We tested containing gypsy and/or Fab7 in different orientations, from copia LTR-retroelement [(enh)copia]. A single or insulator inserted promoter...
Forum domains are stretches of chromosomal DNA that excised from eukaryotic chromosomes during their spontaneous non-random fragmentation. Most forum 50-200 kb in length. We mapped domain termini using FISH on polytene and we performed genome-wide mapping a Drosophila melanogaster genomic tiling microarray consisting overlapping 3 fragments. found very often correspond to regions intercalary heterochromatin late replication chromosomes. contain clusters several or many genes. The largest the...
Any method for silencing the activity of HIV-1 retrovirus should tackle extremely high variability sequences and mutational escape. We studied sequence in vicinity selected RNA interference (RNAi) targets from isolates subtype A Russia, we propose that using artificial RNAi is a potential alternative to traditional antiretroviral therapy. prove multiple overcomes isolates. The optimal number critically depends on conservation target sequences. total are conserved with probability 0.7–0.8...
The high sensitivity of living organic forms to space radiation remains the critical issue during spaceflight, which they will be chronically exposed months interplanetary or even decades interstellar spaceflight. In human body, all actively dividing and poorly differentiated cells are always close being damaged by radiological chemical agents. chronic exposure ionizing primarily causes changes in blood counts intestinal damage such as fibrosis, obliterative vasculitis, gut microbiota,...