A5058543160- Cellular transport and secretion
- Lipid Membrane Structure and Behavior
- Erythrocyte Function and Pathophysiology
- Immunotherapy and Immune Responses
- Ubiquitin and proteasome pathways
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Hemoglobin structure and function
- Whipple's Disease and Interleukins
- Genetic and Kidney Cyst Diseases
- CAR-T cell therapy research
- Genomics and Chromatin Dynamics
- Endoplasmic Reticulum Stress and Disease
- Nuclear Structure and Function
- Renal and related cancers
- Signaling Pathways in Disease
- Virus-based gene therapy research
- Biomedical Research and Pathophysiology
- Cancer Immunotherapy and Biomarkers
- Chemokine receptors and signaling
- interferon and immune responses
- Biochemical and Structural Characterization
- Protist diversity and phylogeny
University of California, Santa Barbara
2011-2020
Institut thématique Génétique, génomique et bioinformatique
2011-2017
Abstract Purpose: We previously reported that autologous dendritic cells pulsed with acid-eluted tumor peptides can stimulate T cell–mediated antitumor immune responses against brain tumors in animal models. As a next step vaccine development, phase I clinical trial was established to evaluate this strategy for its feasibility, safety, and induction of systemic intracranial T-cell patients glioblastoma multiforme. Experimental Design: Twelve were enrolled into multicohort dose-escalation...
Autosomal-dominant (AD) polycystic kidney disease (PKD) is a leading cause of renal failure in the United States, and currently lacks available treatment options to slow progression. Mutations gene coding for polycystin-1 (PC1) underlie majority cases but function PC1 has remained poorly understood. We have previously shown that regulates transcriptional activity signal transducer activator transcription-6 (STAT6). Here we show STAT6 aberrantly activated cyst-lining cells PKD mouse models....
Little is known about the quantitative modulation of major histocompatibility complex (MHC) Class I expression on human gliomas that effected by interferons; even less immunogenic peptides are accommodated in peptide-binding motifs MHC alleles these brain tumors. In this article authors investigated ability interferon (IFN)alpha and IFNgamma to upregulate modulate acid-eluted I-bound glioblastoma multiforme (GBM) cells vitro.Early-passage primary GBM cell cultures U87MG were incubated with...
Syntaxin 3 (Stx3), a SNARE protein located and functioning at the apical plasma membrane of epithelial cells, is required for polarity. A fraction Stx3 localized to late endosomes/lysosomes, although how it traffics there its function in these organelles unknown. Here we report that undergoes monoubiquitination conserved polybasic domain. present basolateral-but not apical-plasma rapidly endocytosed, targeted endosomes, internalized into intraluminal vesicles (ILVs), excreted exosomes....
Syntaxins are a conserved family of SNARE proteins and contain C-terminal transmembrane anchors required for their membrane fusion activity. Here we show that Stx3 (syntaxin 3) unexpectedly also functions as nuclear regulator gene expression. We found alternative splicing creates soluble isoform termed Stx3S, lacking the anchor. Soluble Stx3S binds to import factor RanBP5 (RAN-binding protein 5), targets nucleus, interacts physically functionally with several transcription factors, including...
Abstract Syntaxins are a family of membrane-anchored SNARE proteins that essential components required for membrane fusion in eukaryotic intracellular trafficking pathways. contain an N-terminal regulatory domain, termed the H abc domain is not highly conserved at primary sequence level but folds into three-helix bundle structurally among members. The syntaxin has previously been found to be very similar GAT present GGA members and related otherwise completely unrelated syntaxins. Because...
Abstract Synergistic signaling of IL-15 and IL-21 is important in driving pathogenic T NK cell responses multiple inflammatory diseases. are part the common gamma chain (γc) cytokine family, which use shared γc receptor subunit their complexes. each promote cytolytic activity IFNγ production but can also synergistically enhance function other cytotoxic cytokines. Consequently, blockade or alone likely insufficient to improve disease outcome pathologic environments. EQ102 a multi-cytokine...
SUMMARY Syntaxins - a conserved family of SNARE proteins contain C-terminal transmembrane anchors required for their membrane fusion activity. Here we show that syntaxin 3 (Stx3) unexpectedly also functions as nuclear regulator gene expression. Alternative splicing leads to soluble isoform, termed Stx3S, lacking the anchor. Soluble Stx3S binds import factor RanBP5, targets nucleus and interacts physically functionally with several transcription factors, including ETV4 ATF2. is differentially...
Abstract Syntaxin 3 (Stx3), a SNARE protein located and functioning at the apical plasma membrane of epithelial cells, is required for polarity. A fraction Stx3 localized to late endosomes / lysosomes though how it traffics there its function in these organelles unknown. Here we report that undergoes mono - ubiquitination conserved polybasic domain. present basolateral – but not rapidly endocytosed, targeted endosomes, internalized into intraluminal vesicles (ILVs) excreted exosomes. non...
The uptake and trafficking of cell surface receptors can be monitored by a technique called 'antibody-feeding' which uses an externally applied antibody to label the receptor on cultured, live cells. Here, we adapt traditional antibody-feeding experiment polarized epithelial cells (Madin-Darby Canine Kidney) grown permeable Transwell supports. By adding two tandem extracellular Myc epitope tags C-terminus SNARE protein syntaxin 3 (Stx3), provided site where could bind, allowing us perform...
Abstract Advances in adoptive transfer immunotherapy have found beneficial effects for Interleukin-15 (IL-15) the graft versus tumor (GvT) activity of NK and cytotoxic T cells. However, benefits this must be weighed against increased risk acute host disease (GvHD) patients. We propose that BNZ-2, a peptide antagonist IL-15 IL-21 signaling, as therapeutic option GvHD may relieve intestinal pathologies while retaining activity. As novel model inflammatory disease, we humanization with...
Abstract Syntaxins are a family of membrane-anchored SNARE proteins that essential components required for membrane fusion in all eukaryotic intracellular trafficking pathways. contain an N-terminal regulatory domain, termed the H abc is not highly conserved at primary sequence level but folds into three-helix bundle structurally among members. The syntaxin domain has previously been found to be very similar GAT present GGA members and related otherwise completely unrelated syntaxins....