A5089674354- RNA Interference and Gene Delivery
- Cancer Research and Treatments
- DNA Repair Mechanisms
- Virus-based gene therapy research
- Salmonella and Campylobacter epidemiology
- Telomeres, Telomerase, and Senescence
University of Sheffield
2019-2023
Abstract Salmonella Typhi activates the host DNA damage response through typhoid toxin, facilitating symptoms and chronic infections. Here we reveal a non-canonical response, which call RING (response induced by genotoxin), characterized accumulation of phosphorylated histone H2AX (γH2AX) at nuclear periphery. is result persistent mediated toxin nuclease activity hyperphosphorylation RPA, sensor single-stranded (ssDNA) replication stress. The overloads RPA pathway with ssDNA substrate,...
Abstract The typhoid toxin is a secreted virulence factor of typhoidal serovars the bacterial pathogen Salmonella enterica implicated in fever and chronic infections. causes DNA damage response human cells, characterised by cell-cycle arrest cellular distension, resulting senescence increased burden. To better understand host responses to toxin, we performed transcriptomic analysis intoxicated cells found that induced expression genes relating type-I interferon response, including...
Damage to our genome causes acute senescence in mammalian cells, which undergo growth arrest and release a senescence-associated secretory phenotype (SASP) that propagates the stress response bystander cells. Thus, is powerful tumor suppressor. Salmonella enterica hijacks through its typhoid toxin, usurps unidentified factors secretome of senescent cells mediate intracellular infections. Here, transcriptomics toxin-induced (TxSCs) proteomics their identify as Wnt5a, INHBA, GDF15. Wnt5a...
Damage to our genome causes acute senescence in mammalian cells, which undergo growth arrest and release a secretome that elicits cell cycle bystander cells through the senescence-associated secretory phenotype (SASP). Thus, is powerful tumour suppressor. Salmonella enterica hijacks its typhoid toxin, usurps unidentified factors stress of senescent mediate intracellular infections. Here, transcriptomics toxin-induced (txSCs) proteomics their identified secreted ligands activate TGFβ pathway...