P element-mediated mutagenesis has been used to disrupt an estimated 25% of genes essential for Drosophila adult viability. Mutation all in the fly genome, however, poses a problem, because elements show significant hotspots integration. In addition, advanced screening scenarios often require use element-based tools like generation germ-line mosaics using FLP recombinase-mediated recombination or gene misexpression UAS/Gal4 system. These techniques are and can therefore not be combined with...

Morphogenesis of the Drosophila melanogaster embryo is associated with a dynamic reorganization actin cytoskeleton that mediated by small GTPases Rho family. Often, Rho1 controls different aspects cytoskeletal function in parallel, requiring complex level regulation. We show guanine triphosphate (GTP) exchange factor DRhoGEF2 apically localized epithelial cells throughout embryogenesis. demonstrate DRhoGEF2, which has previously been shown to regulate cell shape changes during gastrulation,...

Cancers caused by high-risk human papillomavirus (HPV) remain a significant health threat resulting in more than 300,000 deaths, annually. Persistent expression of two HPV oncogenes, E6 and E7, are necessary for cancer development progression. has several functions contributing to tumorigenesis one which is blocking programmed cell death, apoptosis. The detailed mechanism anti-apoptosis function not fully understood. Here, using Drosophila model HPV18E6 the UBE3A-induced pathogenesis, we...

Morphogenesis of the Drosophila embryo is associated with dynamic rearrangement actin cytoskeleton mediated by small GTPases Rho family. These act as molecular switches that are activated guanine nucleotide exchange factors. One these factors, DRhoGEF2, plays an important role in constriction filaments during pole cell formation, blastoderm cellularization, and invagination germ layers. Here, we show DRhoGEF2 equally morphogenesis segmental grooves, which become apparent tissue infoldings...

How clathrin‐mediated endocytosis ( CME ) retrieves vesicle proteins into newly formed synaptic vesicles SVs remains a major puzzle. Besides its roles in stimulating clathrin‐coated formation and regulating SV size, the clathrin assembly protein AP180 has been identified as key player retrieving proteins. The mechanisms by which recruits are not fully understood. Here, we show that following acute inactivation of Drosophila , recycling is severely impaired at larval neuromuscular synapse...

The tricellular junction (TCJ) forms at the convergence of bicellular junctions from three adjacent cells in polarized epithelia and is necessary for maintaining transepithelial barrier. In fruitfly Drosophila, TCJ generated meeting point septate junctions. Gliotactin was first identified component development. a member neuroligin family associates with PDZ protein discs large. Beyond this interaction, little known about mechanisms underlying localization function TCJ. study, we show that...

Cervical cancer is one of the leading causes death in women worldwide. The causative agents cervical cancers, high-risk human papillomaviruses (HPVs), cause through action two oncoproteins, E6 and E7. oncoprotein cooperates with an E3 ubiquitin ligase (UBE3A) to target p53 tumour suppressor important polarity junctional PDZ proteins for proteasomal degradation, activities that are believed contribute towards malignancy. However, link between degradation E6-mediated malignancy largely...

Summary Establishment and maintenance of permeability barriers is one the most important functions epithelial cells. Tricellular junctions (TCJs) maintain at contact site three Gliotactin, a member Neuroligin family, only known Drosophila protein exclusively localized to TCJ necessary for barrier. Overexpression triggers spread Gliotactin away from causes cells delaminate, migrate die. Furthermore, excess cell membrane results in an extensive downregulation Discs large (Dlg) septate...

The mammalian MAGI proteins play important roles in the maintenance of adherens and tight junctions. family contains modular domains such as WW PDZ necessary for scaffolding membrane receptors intracellular signaling components. Loss leads to reduced junction stability while overexpression can lead increased adhesion stabilization epithelial morphology. However, how Magi regulates assembly epithelia is largely unknown. We investigated single Drosophila homologue study vivo role development....

ABSTRACT Epithelial bicellular and tricellular junctions are essential for establishing maintaining permeability barriers. Tricellular formed by the convergence of three at corners neighbouring epithelia. Gliotactin, a member Neuroligin family, is located Drosophila junction, crucial formation septate junctions, as well barrier function. Gliotactin protein levels tightly controlled phosphorylation tyrosine residues endocytosis. Blocking endocytosis or overexpressing results in spread from...

Most studies exploring molecular and physiological responses to temperature have focused on constant treatments. To gain a better understanding of the impact fluctuating temperatures, we investigated effects increased variation Phanaeus vindex dung beetles across levels biological organization. Specifically, hypothesized that is energetically demanding. We predicted thermal sensitivity metabolic rate energetic reserves would be reduced with increasing fluctuation. test this, examined (20°C),...

Abstract Human papillomavirus (HPV) is the leading cause of cervical cancer and has been implicated in several other types including vaginal, vulvar, penile, oropharyngeal cancers. Despite recent availability a vaccine, there are still over 310,000 deaths each year worldwide. Current treatments for HPV-mediated cancers show limited efficacy, would benefit from improved understanding disease mechanisms. Recently, we developed Drosophila ‘HPV 18 E6’ model that displayed loss cellular...

High-risk human papillomaviruses (HPVs) 16 and 18 are responsible for more than 70% of cervical cancers majority other HPV-associated world-wide. Current treatments these have limited efficacy, which in turn has resulted disease recurrence poor survival rates advanced stages. Hence, there is a significant need development novel molecularly-targeted therapeutics. This can only be achieved through improved understanding mechanism. Recently, we developed Drosophila model HPV18E6 plus E3...

High-risk human papillomaviruses (HR-HPVs) cause almost all cervical cancers and a significant number of vaginal, vulvar, penile, anal, oropharyngeal cancers. HPV16 18 are the most prevalent types among HR-HPVs together more than 70% Low vaccination rate lack molecularly-targeted therapeutics for primary therapy have led to slow reduction in cancer incidence high mortality rate. Hence, creating new models HPV-induced that can facilitate understanding disease mechanism identification key...