A5109149341- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- 3D Printing in Biomedical Research
- Tissue Engineering and Regenerative Medicine
- Reproductive Biology and Fertility
- Animal Genetics and Reproduction
- Zebrafish Biomedical Research Applications
- RNA modifications and cancer
- Viral Infectious Diseases and Gene Expression in Insects
- Erythrocyte Function and Pathophysiology
- Mesenchymal stem cell research
- Cellular Mechanics and Interactions
- RNA and protein synthesis mechanisms
- Force Microscopy Techniques and Applications
- Genomics and Chromatin Dynamics
- Lipid Membrane Structure and Behavior
- Electrospun Nanofibers in Biomedical Applications
- Congenital heart defects research
European Molecular Biology Laboratory
2024
Heidelberg University
2024
Centro de Investigacion Principe Felipe
2006-2011
Universitat de València
2006
Banco de la República Colombia
2006
National Institute of Neurological Disorders and Stroke
1992
National Institutes of Health
1992
Abstract The generation of induced pluripotent stem cells (iPSC) has enormous potential for the development patient-specific regenerative medicine. Human embryonic (hESC) are able to defend their genomic integrity by maintaining low levels reactive oxygen species (ROS) through a combination enhanced removal capacity and limited production these molecules. Such ROS stems partly from small number mitochondria present in hESC; thus, it was important determine that human iPSC (hiPSC) is...
Early development of mammalian embryos occurs in an environment relative hypoxia. Nevertheless, human embryonic stem cells (hESC), which are derived from the inner cell mass blastocyst, routinely cultured under same atmospheric conditions (21% O(2)) as somatic cells. We hypothesized that O(2) levels modulate gene expression and differentiation potential hESC, thus, we performed profiling hESC maintained normoxic or hypoxic (1% 5% conditions. Our analysis revealed hypoxia downregulates...
The isolation of significant numbers human primordial germ cells at several developmental stages is important for investigations the mechanisms by which they are able to undergo epigenetic reprogramming. Only small these can be obtained from embryos appropriate stages, so differentiation embryonic stem essential obtain sufficient permit examination. Despite progress in enrichment using fluorescence-activated cell sorting (FACS), there still no definitive marker phenotype. Expression widely...
Background Cell culture media conditioned by human foreskin fibroblasts (HFFs) provide a complex supplement of protein and metabolic factors that support in vitro proliferation embryonic stem cells (hESCs). However, the conditioning process is variable with different batches often exhibiting differing capacities to maintain hESCs culture. While recent studies have examined complement media, detailed information regarding component this lacking. Methodology/Principal Findings Using 1H-Nuclear...
Understanding the transcriptional cues that direct differentiation of human embryonic stem cells (hESCs) and human-induced pluripotent to defined functional cell types is essential for future clinical applications. In this study, we have compared profiles haematopoietic progenitors derived from hESCs at various developmental stages a feeder- serum-free method show largest changes occur during first 4 days differentiation. Data mining on basis molecular function revealed Rho-GTPase signalling...
The recent progress in stem cell biology has created new approaches for their study as well application to the treatment of human diseases [1–3]. success stem-cell based technologies clinical setting [4, 5] emphasized need improve standards quality all phases therapy, particularly development culture methods that circumvent products animal origin since these might provoke infections or immune rejection following transplantation patients. Indeed, Martin et al. (2005) [6] demonstrated hESC...
SUMMARY To migrate, divide, and change shape, cells must regulate the mechanics of their periphery. The cell surface is a complex structure that consists thin, contractile cortical actin network tethered to plasma membrane by specialized membrane-to-cortex attachment (MCA) proteins. This active constantly fluctuating system maintains delicate mechanochemical state which permits spontaneous polarization shape when needed. Combining in silico , vitro vivo experiments we show how viscosity MCA...