A5027882281- Cellular Mechanics and Interactions
- Microtubule and mitosis dynamics
- Developmental Biology and Gene Regulation
- Micro and Nano Robotics
- Congenital heart defects research
- Force Microscopy Techniques and Applications
- 3D Printing in Biomedical Research
- Pickering emulsions and particle stabilization
- Theoretical and Computational Physics
- Gene Regulatory Network Analysis
- Diffusion and Search Dynamics
- Wound Healing and Treatments
- Genetic and Kidney Cyst Diseases
- Dermatologic Treatments and Research
- Photosynthetic Processes and Mechanisms
- Biological Research and Disease Studies
- Planarian Biology and Electrostimulation
- Plant Molecular Biology Research
- Genetics, Aging, and Longevity in Model Organisms
- Hedgehog Signaling Pathway Studies
- Fungal and yeast genetics research
- Microfluidic and Bio-sensing Technologies
- Renal and related cancers
- Paleontology and Evolutionary Biology
- Aviation History and Innovations
North Carolina State University
2019-2024
North Central State College
2019-2024
Indiana University Bloomington
2011-2020
Biocom
2012-2020
European Molecular Biology Laboratory
2015-2020
European Molecular Biology Laboratory
2016-2018
University of Helsinki
2018
Universidade Federal do Rio Grande do Sul
2008
Murine dermis contains functionally and spatially distinct fibroblast lineages that cease to proliferate in early postnatal life. Here, we propose a model which negative feedback loop between extracellular matrix (ECM) deposition proliferation determines dermal architecture. Virtual-tissue simulations of our faithfully recapitulate maturation, predicting loss spatial segregation dictating migration is only required for wound healing. To test this, performed
The formation of body segments in vertebrate embryos involves local cell interactions independent cyclic gene expression. [Also see Perspective by Kondo ]
Abstract Morphogenesis in animal tissues is largely driven by actomyosin networks, through tensions generated an active contractile process. Although the network components and their properties are known, networks can be reconstituted vitro, requirements for contractility still poorly understood. Here, we describe a theory that predicts whether isotropic will contract, expand, or conserve its dimensions. This analytical correctly behavior of simulated consisting filaments with varying...
A self-propelled particle model is introduced to study cell sorting occurring in some living organisms. This allows us evaluate the influence of intrinsic motility separately from differential adhesion with fluctuations, a mechanism previously shown be sufficient explain variety rearrangement processes. We find that tendency cells actively follow their neighbors greatly reduces segregation time scales. finite-size analysis process reveals clear algebraic growth laws as physical...
ABSTRACT Cytoskeletal networks of actin filaments and myosin motors drive many dynamic cell processes. A key characteristic these is their contractility. Despite intense experimental theoretical efforts, it not clear what mechanism favors network contraction over expansion. Recent work points to a dominant role for the nonlinear mechanical response filaments, which can withstand stretching but buckle upon compression. Here, we present an alternative mechanism. We study how interactions...
We describe a multi-scale, liver-centric in silico modeling framework for acetaminophen pharmacology and metabolism. focus on computational model to characterize whole body uptake clearance, liver transport phase I II do this by incorporating sub-models that span three scales; Physiologically Based Pharmacokinetic (PBPK) of distribution at the level, cell blood flow tissue/organ level metabolism sub-cellular level. have used standard modalities each scales. In particular, we Systems Biology...
In autosomal dominant polycystic kidney disease (ADPKD), cysts accumulate and progressively impair renal function. Mutations in PKD1 PKD2 genes are causally linked to ADPKD, but how these mutations drive cell behaviors that underlie ADPKD pathogenesis is unknown. Human frequently express cadherin-8 (cad8), expression of cad8 ectopically vitro suffices initiate cystogenesis. To explore behavioral mechanisms cad8-driven cyst initiation, we developed a virtual-tissue computer model. Our...
Abstract The intestinal epithelium is the fastest regenerative tissue in body, fueled by fast‐cycling stem cells. number and identity of these dividing migrating cells are maintained a mosaic pattern at base crypt. How underlying regulatory scheme manages this dynamic cell niche not entirely clear. We stimulated organoids with Notch ligands inhibitors discovered that employ positive feedback mechanism via direct binding to second intron Notch1 gene. Inactivation CRISPR /Cas9 mutation...
While contraction of sarcomeric actomyosin assemblies is well understood, this not the case for disordered networks actin filaments (F-actin) driving diverse essential processes in animal cells. For example, at onset meiosis starfish oocytes a contractile F-actin network forms nuclear region transporting embedded chromosomes to assembling microtubule spindle. Here, we addressed mechanism 3D by comparing quantitative observations computational models. We analyzed chromosome trajectories and...
The intrinsic genetic program of a cell is not sufficient to explain all the cell's activities. External mechanical stimuli are increasingly recognized as determinants behavior. In epithelial folding event that constitutes beginning gastrulation in Drosophila, future mesoderm leads establishment contractile actomyosin network triggers apical constriction cells and thereby tissue folding. However, some do constrict but instead stretch, even though they share same their constricting neighbors....
Cytokinesis, the process that partitions mother cell into two daughter cells, requires assembly and constriction of an equatorial actomyosin network. Different types non-motor F-actin crosslinkers localize to network, but their functional contribution remains poorly understood. Here, we describe a synergy between small rigid crosslinker plastin large flexible spectrin in C. elegans one-cell embryo. In contrast single inhibitions, co-inhibition βH-spectrin (SMA-1) results cytokinesis failure...
In convergent-extension (CE), a planar-polarized epithelial tissue elongates (extends) in-plane in one direction while shortening (converging) the perpendicular direction, with cells both elongating and intercalating along converging axis. CE occurs during development of most multicellular organisms. Current models assume cell or asymmetry, but neglect preferential filopodial activity convergent axis observed many tissues. We propose cell-based model based on asymmetric tension forces...
Somitogenesis is often described using the clock-and-wavefront (CW) model, which does not explain how molecular signaling rearranges pre-somitic mesoderm (PSM) cells into somites. Our scanning electron microscopy analysis of chicken embryos reveals a caudally-progressing epithelialization front in dorsal PSM that precedes somite formation. Signs apical constriction and tissue segmentation appear this layer 3-4 lengths caudal to last-formed somite. We propose mechanical instability model...
Planar cell polarity (PCP) is an important patterning mechanism in both vertebrates and invertebrates by which cells coherently polarize along the apical surface of epithelium. This acts upstream many developmental processes, such as oriented growth, division, movements orientation body hairs. While various models have been proposed to explain PCP patterning, all rely on persistent global cues/gradients obtain large tissues. However, recent experimental work has shown that this process can...
During animal development, the acquisition of three-dimensional morphology is a direct consequence dynamic interaction between cellular forces and mechanical properties cells their environment. While generation transmission has been widely explored, less known about changes in cell during morphogenesis. Here, we characterise spatially map three dimensions dynamics Drosophila gastrulation utilising line-scan Brillouin microscopy. We find that embryo undergo rapid varying these differ...
Summary Morphogenesis in animal tissues is largely driven by tensions of actomyosin networks, generated an active contractile process that can be reconstituted vitro . Although the network components and their properties are known, requirements for contractility still poorly understood. Here, we describe a theory predicts whether isotropic will contract, expand, or conserve its dimensions. This analytical correctly behavior simulated networks consisting filaments with varying combinations...
Abstract Cytoskeletal networks of actin filaments and myosin motors drive many dynamic cell processes. A key characteristic these is their contractility. Despite intense experimental theoretical efforts, it not clear what mechanism favors network contraction over expansion. Recent work points to a dominant role for the nonlinear mechanical response filaments, which can withstand stretching but buckle upon compression. Here we present an alternative mechanism. We study how interactions...
Abstract The intrinsic genetic programme of a cell is not sufficient to explain all the cell’s activities. External mechanical stimuli are increasingly recognized as determinants behaviour. In epithelial folding event that constitutes beginning gastrulation in Drosophila , future mesoderm leads establishment contractile actomyosin network triggers apical constriction cells, and thereby, tissue folding. However, some cells do constrict but instead stretch, even though they share same their...