A5073574474- Ultrasound and Hyperthermia Applications
- Photoacoustic and Ultrasonic Imaging
- Ultrasound and Cavitation Phenomena
- Ultrasound Imaging and Elastography
- Coronary Interventions and Diagnostics
- Nanoplatforms for cancer theranostics
- Advanced MRI Techniques and Applications
- Ultrasonics and Acoustic Wave Propagation
- Venous Thromboembolism Diagnosis and Management
- Thermoregulation and physiological responses
- Fluid Dynamics and Mixing
- Blood properties and coagulation
- Peripheral Artery Disease Management
- Angiogenesis and VEGF in Cancer
- Acute Ischemic Stroke Management
- Ultrasound in Clinical Applications
- Geomagnetism and Paleomagnetism Studies
- Inertial Sensor and Navigation
- MRI in cancer diagnosis
- Nanoparticle-Based Drug Delivery
- Digital Innovation in Industries
- Flexible and Reconfigurable Manufacturing Systems
- Cardiac Valve Diseases and Treatments
- Virus-based gene therapy research
- Seismic Waves and Analysis
Sunnybrook Research Institute
2015-2025
Sunnybrook Hospital
2012-2025
University of Toronto
2015-2024
Sunnybrook Health Science Centre
2013-2024
Case Western Reserve University
2024
Health Sciences Centre
2002-2024
American Institute of Ultrasound in Medicine
2019
RWTH Aachen University
2017
Helios Hospital Siegburg
2015
University Hospital Bonn
2015
Porphyrin-phospholipid conjugates were used to create photonic microbubbles (MBs) having a porphyrin shell ("porshe"), and their acoustic photoacoustic properties investigated. The inclusion of porphyrin-lipid in the MB increased yield, improved serum stability, generated narrow volumetric size distribution with peak 2.7 ± 0.2 μm. Using an model, we calculated porshe stiffness be 3-5 times greater than that commercial lipid MBs. Porshe MBs found intrinsically suitable for both ultrasound...
It was previously shown that it is possible to produce nonlinear scattering from microbubble contrast agents using transmit frequencies in the 14-32 MHz range, suggesting possibility of performing high-frequency, imaging. In this study, we describe development B-scan imaging instrumentation capable operating at center between 10 and 50 MHz. The system underwent validation experiments 20 30 Agent characterization demonstrate presence for conditions used study. Using wall-less vessel phantoms,...
An air bubble driven by ultrasound can become shape-unstable through a parametric instability. We report time-resolved optical observations of shape oscillations (mode n=2 to 6) micron-sized single bubbles. The observed mode number n was found be linearly related the ambient radius bubble. Above critical driving pressure threshold for oscillations, which is minimal at resonance volumetric radial mode, independent forcing amplitude. microbubble were also analyzed numerically introducing small...
Objectives: To characterize the ability of high-intensity focused ultrasound to achieve thrombolysis in vitro and investigate feasibility this approach as a means restoring blood flow thrombus-occluded arteries vivo. Materials Methods: All experiments were approved by Institutional Animal Care Committee. Thrombolysis was performed with 1.51-MHz transducer pulse lengths 0.1 10 milliseconds acoustic powers up 300 W. In clots formed from rabbit arterial situated 2-mm diameter tubing. Both...
Objective: We sought to investigate feasibility of vasa vasorum imaging using the novel technique contrast harmonic intravascular ultrasound. Methods: Prototype ultrasound (IVUS) instrumentation was developed for sensitive detection microbubble agents. The technique, "harmonic" imaging, involves transmitting at 20 MHz (fundamental) and detecting signals 40 (second harmonic). Phantom experiments were conducted a small vessel in wall surrounding larger vessel. In vivo atherosclerotic rabbit...
Ultrasound stimulated microbubbles (USMB) are being investigated for their potential to promote the uptake of anticancer agents into tumor tissue by exploiting ability enhance microvascular permeability. At sufficiently high ultrasound transmit amplitudes it has also recently been shown that USMB treatments can, on own, induce vascular damage, shutdown blood flow, and inhibit growth. The objective this study is examine antitumor effects 'antivascular' in conjunction with chemotherapy, which...
Ultrasound and photoacoustic imaging are highly complementary modalities since both use ultrasonic detection for operation. Increasingly, ultrasound have been integrated in terms of hardware instrumentation. To generate a broadly accessible dual-modality contrast agent, we generated microbubbles (a standard agent) solution methylene blue dye). This MB 2 was formed effectively optimized as solution. As microbubble concentration increased (with constant), signal attenuated the When held no...
It is estimated that only 2–6% of patients receive thrombolytic therapy for acute ischemic stroke suggesting alternative therapies are necessary. In this study, we investigate the potential high intensity focused ultrasound (HIFU) to initiate thrombolysis in an embolic model stroke. Iron-loaded blood clots were injected into middle cerebral artery (MCA) New Zealand White rabbits, through internal carotid and blockages confirmed by angiography. MRI was used localize iron-loaded clot target...
Considerable effort is being directed toward investigating the use of ultrasound (US) stimulated microbubbles (MB) to promote uptake anticancer agents in tumors. In this study we propose and investigate a new method for combining therapeutic with agents, which induce antivascular effects combine these an antiangiogenic treatment strategy, case metronomic chemotherapy. This effectively vascular targeting rather than drug delivery approach. Experiments were conducted on MDA-MB-231 breast...
The future of nanomedicines in oncology requires leveraging more than just the passive drug accumulation tumors through enhanced permeability and retention effect.Promising results combining mild hyperthermia (HT) with lyso-thermosensitive liposomal doxorubicin (LTSL-DOX) has led to improved delivery potent antitumor effects pre-clinical studies.The ultimate patient benefit from these treatments can only be realized when robust methods HT achieved clinically.One most promising non-invasive...
There is a growing interest in microbubble based ultrasound contrast imaging applications the 5-15 MHz range. In this study, individual microbubbles were insonified at low pressures (≤ 25 kPa) using an "acoustic spectroscopy" approach which entailed transmitting sequence of tone bursts with center frequencies ranging from 4 to 13.5 MHz. The fundamental (transmit) frequency radial excursion amplitude was calculated scattered signals produce resonance curve for each bubble. For diameters...
Focused ultrasound with microbubbles is an emerging technique for blood-brain barrier opening. Here, a comprehensive theoretical model of bubble-fluid-vessel system has been developed which accounts the bubble's nonspherical oscillations inside microvessel, and its resulting acoustic emissions. Numerical simulations unbound confined encapsulated bubbles were performed to evaluate effect vessel wall on emissions stresses. Using Marmottant shell model, normalized second harmonic fundamental...
Ultrasound-activated nanobubbles are being widely investigated as contrast agents and therapeutic vehicles. Nanobubbles hold potential for accessing the tumor extravascular compartment, though this relies on clinically debated passive accumulation which evidence to date is indirect. We recently reported ultrasound-triggered conversion of high payload porphyrin-encapsulated microbubbles nanobubbles, with actively enhanced permeability local delivery. This platform holds implications...
Background: There has been growing interest in nanobubbles for their potential to extend bubble-mediated ultrasound approaches beyond that of larger microbubble counterparts. In particular, the smaller scale may enable them access tumor extravascular compartment imaging and therapy closer proximity cancer cells. Compelling preliminary demonstrations therapeutic abilities have thus emerged, with emphasis on ability extravasate. However, studies date rely indirect histologic evidence cannot...
Delivery of viral vectors as gene therapies to treat neurodegenerative diseases has been hampered by the inability penetrate blood brain barrier (BBB) and invasive or non-targeted delivery options prone inducing immune responses. MR guided focused ultrasound (MR-g-FUS) microbubbles have demonstrated safe, temporary, targeted BBB permeabilization clinically.We developed clinically scalable, microbubble drug conjugates (MDCs) for therapy, AAV.SIRT3-myc [adeno-associated virus expressing...