A5054964415- Entomopathogenic Microorganisms in Pest Control
- Insect Resistance and Genetics
- Insect and Arachnid Ecology and Behavior
- Insect symbiosis and bacterial influences
- Parasite Biology and Host Interactions
- Forest Insect Ecology and Management
- Plant and animal studies
- Viral Infectious Diseases and Gene Expression in Insects
- Data-Driven Disease Surveillance
- Insect-Plant Interactions and Control
- Neurobiology and Insect Physiology Research
- Nematode management and characterization studies
- Genetics, Bioinformatics, and Biomedical Research
- Chromosomal and Genetic Variations
- Fossil Insects in Amber
- Evolution and Genetic Dynamics
- Microtubule and mitosis dynamics
- Data Analysis with R
- Misinformation and Its Impacts
- Insect behavior and control techniques
- Scientific Computing and Data Management
- Zoonotic diseases and public health
- Plant and fungal interactions
- Insect and Pesticide Research
- Evolutionary Game Theory and Cooperation
Harvard University
2022-2025
University of Otago
2021-2024
University of Georgia
2021
Northwest A&F University
2017-2018
NILU
2015
Norwegian University of Life Sciences
2015
Abstract In the United States, Endometrial carcinoma (EC) is most frequently occurring gynecologic cancer. Many ECs harbor mutations in cell cycle regulatory genes including TP53 and RB1, amongst others. RB p53 both regulate G1/S transition while also regulates G2/M mitotic progression, all of which rely on targetable kinases. It likely that many defects some aspect regulation, but there has been no profiling p53- or RB- linked functional capacity corresponding therapeutic vulnerabilities EC...
<p>Supplementary Figure S22</p>
<p>Aurora kinase B inhibitor sensitive endometrial carcinoma cells reveal more rapidly decreasing percentages of mitotic than resistant in the setting Aurora inhibition. <b>A,</b> A cartoon demonstrating experimental setup is shown. <b>B,</b> HEC1B and ARK1 were treated with vehicle (DMSO) or a dose curve CDK1 (CDK1i) Ro-3306 for 16 hours. The then underwent bromodeoxyuridine (BrdU)/propidium iodide (PI) cell cycle flow cytometry profiling. Shown here are bar...
<p>Supplementary Figure S13</p>
<p>Supplementary Figure S1</p>
<p>Supplementary Figure S12</p>
<p>Supplementary Figure S10</p>
<p>Endometrial carcinomas with transcriptionally evident mitotic spindle organization or progression regulatory defects are sensitive to Aurora Kinase B inhibition. <b>A,</b> Cartoon illustrating the different kinases governing G2/M transition and what part of mitosis inhibitors these target (Aurora kinase A (AURKA), (AURKB), Polo-like 1 (PLK1)). <b>B</b> <b>C,</b> Protein lysates were prepared from untreated cell lines (<b>B</b>)...
<p>Supplementary Figure S19</p>
<p>Supplementary Figure S6</p>
<p>RB and some form of p53 are expressed in the majority endometrial carcinoma cells regardless genomic status but have varying regulatory or functional ability. <b>A</b> <b>B,</b> Protein lysates were prepared from untreated cell lines (left each panel) organoid (right analyzed by western blot. In <b>A</b>, membranes for type probed RB, stripped re-probed tubulin as a loading control. B, p53, then <b>C</b> <b>D,</b> Organoids...
<p>Immunohistochemical, genomic, functional/regulatory, and related therapeutic sensitivity status of p53 RB in endometrial carcinoma models</p>
<p>Endometrial carcinoma cell sensitivity to G1/S targeted therapies correlates with RB regulatory status. <b>A</b> and <b>B,</b> Protein lysates were prepared from untreated lines (left in each panel) organoid (right analyzed by western blot. In <b>A</b>, membranes for type probed CDK4, stripped re-probed CDK6, tubulin as a loading control. <b>B</b>, Cyclin E1, then An arrow indicates the main E1 isoform, below that, low molecular weight...
<p>RB intact regulation and mitotic progression deficient endometrial carcinoma cells demonstrate different responses to cell cycle targeting therapies <i>in vivo</i>. <b>A,</b> Cartoon demonstrating vivo</i> study experimental setup. For each study, after treatment initiation, mice were euthanized once they developed signs of morbidity; then ascites volume was obtained, gross photos taken, residual tumor harvested for all animals possible....
<p>Supplementary Figure S3</p>
<p>Supplementary Figure S20</p>
<p>This file contains Supplementary Materials and Methods along with accompanying References.</p>
<p>Aurora kinase B inhibitor sensitive endometrial carcinoma cells show more rapidly decreasing percentages of mitotic than resistant models in the setting nocodazole-induced microtubule instability. <b>A,</b> A cartoon demonstrating experimental setup is shown. <b>B,</b> HEC1B, ARK1, and AN3CA were treated for 24 hours with vehicle (DMSO) or a dose curve nocodazole (Noc) then underwent bromodeoxyuridine (BrdU)/propidium iodide (PI) flow cytometry cell cycle...
<div>Abstract<p>In the United States, Endometrial carcinoma (EC) is most frequently occurring gynecologic cancer. Many ECs harbor mutations in cell cycle regulatory genes including <i>TP53</i> and <i>RB1</i>, amongst others. RB p53 both regulate G1/S transition while also regulates G2/M mitotic progression, all of which rely on targetable kinases. It likely that many defects some aspect regulation, but there has been no profiling p53- or RB- linked...
<p>Supplementary Figure S11</p>
<p>Supplementary Figure S2</p>
<p>Supplementary Figure S14</p>
<p>Supplementary Figure S9</p>
<p>Supplementary Figure S8</p>
<p>Supplementary Figure S18</p>