A5047377123- Photosynthetic Processes and Mechanisms
- RNA modifications and cancer
- Mitochondrial Function and Pathology
- RNA and protein synthesis mechanisms
- RNA Research and Splicing
- Cancer-related molecular mechanisms research
- Lipid metabolism and biosynthesis
- Genomics and Phylogenetic Studies
- Plant Molecular Biology Research
- HVDC Systems and Fault Protection
- Plant Reproductive Biology
- CRISPR and Genetic Engineering
- Genetics, Aging, and Longevity in Model Organisms
Hebrew University of Jerusalem
2019-2023
Mitochondria serve as major sites of ATP production and play key roles in many other metabolic processes that are critical to the cell. As relicts an ancient bacterial endosymbiont, mitochondria contain their own hereditary material (i.e. mtDNA, or mitogenome) a machinery for protein biosynthesis. The expression mtDNA plants is complex, particularly at post-transcriptional level. Following transcription, polycistronic pre-RNAs undergo extensive modifications, including trimming, splicing...
Mitochondria play key roles in cellular-energy metabolism and are vital for plant-life, such as successful germination early-seedling establishment. Most mitochondria contain their own genetic system (mtDNA, mitogenome), with an intrinsic protein-synthesis machinery. Although the challenges of maintaining prokaryotic-type structures functions common to Eukarya, land plants possess some most complex organelle composition all known organisms. Angiosperms mtDNAs characteristically largest least...
Mitochondrial biogenesis relies on nuclearly encoded factors, which regulate the expression of organellar-encoded genes. Pentatricopeptide repeat (PPR) proteins constitute a major gene family in angiosperms that are pivotal many aspects mitochondrial (mt)RNA metabolism (e.g. trimming, splicing, or stability). Here, we report analysis MITOCHONDRIA STABILITY/PROCESSING PPR FACTOR1 (MSP1, At4g20090), canonical protein is necessary for functions and embryo development. Loss-of-function allele...
SUMMARY Group‐II introns are self‐splicing mobile genetic elements consisting of catalytic intron‐RNA and its related intron‐encoded splicing maturase protein cofactor. sequences particularly plentiful within the mitochondria land plants, where they reside many critical gene loci. During evolution, plant organellar have degenerated, such as lack regions that required for splicing, also lost their evolutionary proteins. Instead, in plants rely on different host‐acting cofactors, which may...
Cellular respiration involves complex organellar metabolic activities that are pivotal for plant growth and development. Mitochondria contain their own genetic system (mitogenome, mtDNA), which encodes key elements of the respiratory machinery. Plant mtDNAs notably larger than counterparts in Animalia, with genome organization gene expression characteristics. The maturation mitochondrial transcripts extensive RNA editing, trimming splicing events. These essential processing steps rely on...
Mitochondria play key roles in cellular energy metabolism eukaryotes. of most organisms contain their own genome and specific transcription translation machineries. The expression angiosperm mtDNA involves extensive RNA-processing steps, such as RNA trimming, editing, the splicing numerous group II-type introns. Pentatricopeptide repeat (PPR) proteins are players plant organelle gene metabolism. In present analysis, we reveal function MITOCHONDRIAL SPLICING FACTOR 2 (MISF2, AT3G22670) show...
Mitochondria play key roles in cellular energy metabolism within eukaryotic cells. As relics of endosymbiotic bacteria, most (but not all) mitochondria contain their own genome (mitogenome, mtDNA), as well intrinsic biosynthetic machinery for making organelle RNAs and proteins. The expression the mtDNA requires regulated its transcriptome by nuclear-encoded factors. Post-transcriptional mtRNA modifications a central role plant mitogenomes, hence organellar biogenesis physiology. Despite...
Abstract Group II introns are particularly plentiful within plant mitochondrial genomes (mtDNAs), where they interrupt the coding-regions of many organellar genes, especialy complex I (CI) subunits. Their splicing is essential for biogenesis respiratory system and facilitated by various protein-cofactors that belong to a diverse set RNA-binding cofactors. These including maturases, which co-evolved with their host-introns, trans -acting factors, such as members pentatricopeptide-repeat (PPR)...
Mitochondria play key roles in cellular energy metabolism eukaryotes. of most organisms contain their own genome and specific transcription translation machineries. The expression angiosperm mtDNA involves extensive RNA-processing steps, such as RNA trimming, editing, the splicing numerous group II-type introns. Pentatricopeptide repeat (PPR) proteins are players plant organelle gene metabolism. In present analysis, we reveal function MITOCHONDRIAL SPLICING FACTOR 2 (MISF2, AT3G22670) show...
Long intergenic non-coding RNAs (lincRNAs) are transcripts longer than 200 nucleotides that transcribed from loci yet undergo biosynthesis similar to coding mRNAs. The disproportional number of lincRNAs expressed in testes suggests important during gametogenesis, but experimental evidence has implicated very few this process. We took advantage the relatively limited genome nematode Caenorhabditis elegans systematically analyse functions meiosis. deleted six lincRNA genes highly and...
Summary Mitochondria are the main source of ATP production and also contribute to many other processes central cellular function. Mitochondrial activities have been linked with growth, differentiation aging. As relicts bacterial endosymbionts, these organelles contain their own genetic system (i.e., mitogenome or mtDNA). The expression mtDNA in plants is complex, particularly at posttranscriptional level. Following transcription, polycistronic pre-RNAs plant mitochondria processed into...
Summary Group II introns are large catalytic RNAs that particularly prevalent in the organelles of terrestrial plants. In angiosperm mitochondria, group reside coding-regions many critical genes, and their excision is essential for respiratory-mediated functions. Canonical self-splicing mobile genetic elements, consisting intron-RNA its cognate intron-encoded endonuclease factor ( i.e. maturase, Pfam-PF01348). Plant organellar extremely degenerate, lack regions splicing, including related...
ABSTRACT Summary Mitochondria are semi-autonomous organelles that serve as hubs for aerobic energy metabolism. The biogenesis of the respiratory (OXPHOS) system relies on nuclear-encoded factors, which regulate transcription, processing and translation mitochondrial (mt)RNAs. These include proteins primordial origin, well eukaryotic-type RNA-binding families recruited from host genomes to fu nc tion in mitogenome expression. Pentatricopeptide repeat (PPR) constitute a major gene-family...