A5031964869- CRISPR and Genetic Engineering
- Genetics, Aging, and Longevity in Model Organisms
- Cancer-related molecular mechanisms research
- RNA Research and Splicing
- DNA Repair Mechanisms
- RNA modifications and cancer
Hebrew University of Jerusalem
2019-2023
During meiosis, a series of evolutionarily conserved events allow for reductional chromosome division, which is required sexual reproduction. Although individual meiotic processes have been extensively studied, we currently know far less about how meiosis regulated and coordinated. In the Caenorhabditis elegans gonad, mitogen-activated protein kinase (MAPK) signaling drives oogenesis while undergoing spatial activation deactivation waves. However, it unclear MAPK governed facilitates...
Abstract During meiosis, gene expression is silenced in aberrantly unsynapsed chromatin and heterogametic sex chromosomes. Initiation of chromosome silencing disrupted meiocytes with chromosome-autosome translocations. To determine whether this due to aberrant synapsis or loss continuity chromosomes, we engineered Caenorhabditis elegans nematodes non-translocated, bisected X In early mutant males hermaphrodites, segments are enriched euchromatin assembly markers active RNA polymerase II...
Long intergenic non-coding RNAs (lincRNAs) are transcripts longer than 200 nucleotides that transcribed from loci yet undergo biosynthesis similar to coding mRNAs. The disproportional number of lincRNAs expressed in testes suggests important during gametogenesis, but experimental evidence has implicated very few this process. We took advantage the relatively limited genome nematode Caenorhabditis elegans systematically analyse functions meiosis. deleted six lincRNA genes highly and...
Long intergenic non-coding RNAs (lincRNAs) are transcripts longer than 200 nucleotides which transcribed from regions that do not overlap with protein coding sequences. Reproductive organs express high levels of lincRNAs, yet removal many lincRNA genes and dynamic germline expression did lead to fertility defects. It was previously suggested this stems redundant roles different genes. We reported engineering C. elegans strains in we deleted the gonad. The individual mutations major effects...
Abstract Successful tissue homeostasis depends on a balance between proliferation and differentiation. Two PUF proteins, FBF-1 FBF-2, maintain stem-cells in C. elegans germ-cells by binding destabilizing transcripts which promote meiotic entry. However, it is unclear how meiosis initiates because the FBF are also present at significant levels late proliferative early cells. We found that three long-intergenic-non-coding RNAs (lincRNAs) bind proteins timely Deletion of lincRNA genes leads to...
During meiosis of heterogametic cells, such as XY meiocytes, sex chromosomes many species undergo transcriptional silencing known meiotic chromosome inactivation (MSCI). Silencing also occurs in aberrantly unsynapsed autosomal chromatin. The chromatin, is assumed to be the underline mechanism for MSCI. Initiation MSCI disrupted meiocytes with chromosome-autosome translocations. Whether this due aberrant synapsis or lack integrity has never been determined. To address this, we used CRISPR...