A5058585666- Aquaculture Nutrition and Growth
- Aquaculture disease management and microbiota
- Reproductive biology and impacts on aquatic species
- Animal Genetics and Reproduction
- Physiological and biochemical adaptations
- Fish Ecology and Management Studies
- Metabolism and Genetic Disorders
- Virus-based gene therapy research
- Muscle Physiology and Disorders
- Ion channel regulation and function
- RNA Interference and Gene Delivery
- Invertebrate Immune Response Mechanisms
- Insect Utilization and Effects
- Liver physiology and pathology
- Cardiac electrophysiology and arrhythmias
- Molecular Biology Techniques and Applications
- Cancer-related molecular mechanisms research
- Animal Nutrition and Physiology
- Fish Biology and Ecology Studies
- Organ Transplantation Techniques and Outcomes
- Animal Virus Infections Studies
- Nutrition, Genetics, and Disease
- Amino Acid Enzymes and Metabolism
- CRISPR and Genetic Engineering
- Ion Transport and Channel Regulation
Agricultural Research Service
2011-2025
United States Department of Agriculture
2001-2022
Florida Keys Community College
2020
American Fisheries Society
2020
John Wiley & Sons (United States)
2020
Idaho Department of Fish and Game
2017
Experimental Station
2015-2016
Hager Sharp (United States)
2006-2012
National Center for Cool and Cold Water Aquaculture
2007
University of Idaho
2005
Abstract Bile is a yellow‐green liquid produced in the liver from cholesterol and stored gallbladder of vertebrates. improves efficacy lipid digestion by acting as an emulsifier essential activating bile salt lipase, enzyme that has broad substrate specificity. absorption soluble nutrients while also facilitating excretion toxic metabolites, particularly bilirubin. Dietary fishmeal alternatives often disturb acid status fish resulting either increased excretion/decreased intestinal...
We have cloned and characterized the expression of a Kv1.5 K+ channel (cKv1.5) from canine colonic smooth muscle. The amino acid sequence displayed high level identity to other channels class in core region between transmembrane segments S1-S6; however, decreased 74 82% NH2 COOH terminal segments, suggesting that cKv1.5 is distinct isoform class. Functional oocytes demonstrated highly selective for K+, which activates voltage-dependent manner on depolarization membrane potentials positive...
Alternative sources of fish oil (FO) are one the major problems in aquaculture; therefore, goal present study was to examine insect (black soldier fly larvae) (BSLO) as a potential replacer fish/soy juvenile rainbow trout (initial average weight 32 ± 0.15 g) feed. Four diets were formulated wherein FO (control diet) completely replaced with either soybean (SO) or BSLO, and an additional BSLO-based diet supplemented 1.5% bile acid (BSLO + BA) fed for 10 weeks. Growth performance BSLO group...
Recent advances in genotyping-by-sequencing have enabled genome-wide association studies nonmodel species including those aquaculture programs. As with other species, rainbow trout and steelhead (Oncorhynchus mykiss) are susceptible to disease outbreaks can lead significant losses. Fish culturists therefore been pursuing strategies prevent losses common pathogens such as Flavobacterium psychrophilum (the etiological agent for bacterial cold water [CWD]) infectious hematopoietic necrosis...
A cDNA (CSMK1) encoding a delayed rectifier K+ channel of the Kv1.2 class was cloned from canine colonic circular smooth muscle and expressed in Xenopus oocytes. These channels appear to be uniquely gastrointestinal muscles may participate electrical slow wave activity. Functional expression CSMK1 oocytes demonstrated current that activated voltage-dependent manner upon depolarization. This highly sensitive 4-aminopyridine (IC50, 74 microM). low-conductance identified inside-out patches...
Eight strains of rainbow trout were introgressed to develop a single strain (H-ARS) that was selected for faster growth when fed fishmeal-free, plant-based diet (Selection Diet). For four generations, families from these crosses the Selection Diet and increased weight gain. Growth nutrient retention compared among H-ARS two parental strains, House Creek (HSC) Fish Lake (FL) either fish meal or 12 weeks. There significant effect (P < 0.01), but not on gain, interaction by 0.05). The gained...
Rainbow trout (23.1 ± 0.4 g) were fed either a fishmeal- or plant-based diet supplemented with various levels of zinc (0, 15, 30, 60 120 mg kg−1) for 12 weeks. Trout the fishmeal had significantly higher weight gain than diet. Zinc supplementation in no effect on growth performance, suggesting that additional dietary is not required. However, diet, increased up to 30 kg−1 after which was affected. containing exhibited severe retardation, and fish 0 15 diets, cataracts present. Use...
Mice lacking the enzyme fumarylacetoacetate hydrolase (FAH) have symptoms similar to humans with disease hereditary tyrosinemia type I (HT1). FAH-deficient mice were injected a first-generation adenoviral vector expressing human FAH gene and followed for up 9 months. Nontreated mutant control died within 6 weeks from fulminant liver failure, whereas adenovirus-infected animals survived until sacrifice at 2-9 Nine of 13 virus-treated developed hepatocellular cancer. Immunohistochemical...
Previously, this lab has reported the use of hepatocyte transplantation and in vivo gene therapy for correction a mouse model Hereditary Tyrosinemia Type I (HT1). Here, we demonstrate repopulation fumarylacetoacetate hydrolase (FAH)-deficient livers with cultured hepatocytes. Correction disease phenotype was achieved by retrovirally transducing FAH¯ hepatocytes ex vivo, followed selective repopulation. Treated mice were phenotypically normal had corrected plasma amino acid levels liver...
To determine the effects of feed processing method (extrusion or expansion followed by compression pelleting) on physicochemical characteristics, fecal stability, water quality, and growth performance in rainbow trout, three types pellets (expanded sinking [EpS], extruded [ExS], floating [ExF]) were prepared, analyzed, fed to juvenile trout (initial weight = 285.8 ± 15.2 g) at feeding rates based rate curves developed Snake River Farm (Buhl, Idaho, USA) (median [%BW] for 1: 0.85; 2: 0.95; 3:...