A5092902300- CRISPR and Genetic Engineering
- Bacteriophages and microbial interactions
- RNA and protein synthesis mechanisms
University of California, Berkeley
2023-2025
Abstract Bacteriophages constitute one of the largest sources unknown gene content in biosphere. Even for well-studied model phages, robust experimental approaches to identify and study their essential genes remain elusive. We uncover exploit conserved vulnerability phage transcriptome facilitate genome-wide protein expression knockdown via programmable RNA-binding dRfxCas13d (CRISPRi-ART) across diverse phages host. Establishing first broad-spectrum functional genomics platform, we predict...
Abstract Bacteriophages constitute one of the largest reservoirs genes unknown function in biosphere. Even well-characterized phages, functions most remain unknown. Experimental approaches to study phage gene fitness and at genome scale are lacking, partly because phages subvert many modern functional genomics tools. Here we leverage RNA-targeting dCas13d selectively interfere with protein translation measure a transcriptome-wide scale. We find CRISPR Interference through Antisense...