The crustacean gill is a multi-functional organ,and it the site of number physiological processes, including ion transport, which basis for hemolymph osmoregulation; acid-base balance; and ammonia excretion. also by many toxic metals are taken up aquatic crustaceans, thus plays an important role in toxicology these species. This review provides comprehensive overview ecology, physiology, biochemistry, molecular biology mechanisms osmotic ionic regulation performed gill. current concepts...

Na + -K -ATPase and /Ca 2+ exchange activities were studied in gills of Carcinus maenas seawater (SW) after transfer to dilute (DSW). Carcinushyperregulates its hemolymph osmolarity through active uptake , Cl − Ca . In DSW total activity posterior quadrupled; specific was unaffected, increased 1.67-fold. Short-circuit current ( I sc ) voltage-clamped gill hemilamellae −181 μA/cm 2 SW −290 up 90% ouabain sensitive; conductivity similar or (42 46 mS/cm respectively) representative a leaky...

From the marine sponge Halichondria panicea a lectin was isolated and characterized. The homogeneous (composed of protein to 80.7% neutral carbohydrates 14.1%) had molecular weight 78,000 (determined by gel filtration) consisted four subunits with 21,000 each electrophoresis in presence sodium dodecyl sulfate). hemagglutinating activity only slightly dependent upon ionic strength incubation temperature did not require divalent cations, but it inhibited reagents for thiol groups. completely...

ABSTRACT A procedure was developed for the preparation of inside-out vesicles from plasma membranes isolated branchial epithelium green shore crab Carcinus maenas (L.). Procedures normally applied to fish required introduction an additional hypotonic shock obtain a containing 22% vesicles, 33% right-side-out and 45% leaky membrane fragments. In such preparations, first direct evidence uphill (against [Ca2+] gradient) ATP-dependent Na+-gradient-dependent Ca2+ transport in crustacean gills...

ABSTRACT Sodium and chloride fluxes, as well transbranchial potentials (TBP) were studied in isolated perfused gill filaments of the crab Carcinus mediterraneus. Experiments carried out media that either hyposmotic to perfusion solution (asymmetrical conditions) or isosmotic (symmetrical conditions). Fluxes found be diffusional gills under asymmetrical conditions; amiloride induced an inhibitory effect on influxes, without affecting TBP. Under symmetrical conditions, TBP was −7·6 ±2·3 mV,...

Abstract The effects of inhibitors on the efflux ammonia (from basolateral to apical side, J b→a ) were studied in preparation isolated Carcinus gills immersed dilute seawater (DSW) that was identical perfusion solution. Adding 10 −4 M amiloride solution bathing gill preparations reduces by 29% relative control value. Under experimental conditions, it appears only about 1% amiloride‐sensitive influx Na + (J a→b can be exchanged with NH an equimolar basis. ammonium ion is apparently...

Abstract In recent years a convincing body of knowledge has been accumulated indicating transport mechanisms for osmolytes in marine and brackish water Crustacea. Cuticle epithelium cell layers serve as selective communications the exchange ions small organic molecules between crustacean gills their environment. The following apparent permeability coefficients Carcinus posterior gill epithelia was measured: P Rb : Na Cl = 1.7 1.0 0.1. addition to presence ouabain sensitive unidirectional (J...

Unfolded epipodite isolated from American lobsters (Homarus americanus) acclimated to dilute seawater was mounted in an Ussing-type chamber for ion transport studies. The split is electrically polarized, one-cell-layer epithelium supported with cuticle. Under open-circuit conditions, the transepithelial potential -4.2+/-1.0 mV (N=38). In short-circuited epithelium, current averaged over all of preparations -185.4+/-20.2 A cm(-2) (N=38) a high conductance 55.2+/-11.4 mS (N=38), typical leaky...

MEPS Marine Ecology Progress Series Contact the journal Facebook Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout JournalEditorsTheme Sections 120:169-174 (1995) - doi:10.3354/meps120169 Seasonal changes of copper level in shore crabs Carcinus mediterraneus Devescovi, M., Lucu, C. content crab Csrn. (whole crab, haemolymph, hepatopancreas and exoskeleton) were studied establish patterns examine fluctuations population. A significant decrease...