A5012600100- CRISPR and Genetic Engineering
- SARS-CoV-2 and COVID-19 Research
- Monoclonal and Polyclonal Antibodies Research
- Advanced biosensing and bioanalysis techniques
- RNA and protein synthesis mechanisms
- Animal Virus Infections Studies
- RNA Interference and Gene Delivery
- RNA modifications and cancer
- Bacillus and Francisella bacterial research
University of California, Berkeley
2025
The University of Texas at Austin
2021-2025
DEVCOM Army Research Laboratory
2023
United States Army Combat Capabilities Development Command
2023
Abstract Mobile genetic elements evade CRISPR–Cas adaptive immunity by encoding anti-CRISPR proteins (Acrs). Acrs inactivate systems via diverse mechanisms but generally coevolve with a narrow subset of Cas effectors that share high sequence similarity. Here, we demonstrate AcrIIA11 inhibits Streptococcus pyogenes (Sp), Staphylococcus aureus (Sa), and Francisella novicida (Fn) Cas9s in vitro human cells. Single-molecule imaging reveals hinders SaCas9 target search reducing its diffusion on...
The SARS-CoV-2 spike protein is a critical component of vaccines and target for neutralizing monoclonal antibodies (nAbs). Spike also undergoing immunogenic selection with variants that increase infectivity partially escape convalescent plasma. Here, we describe Display, high-throughput platform to rapidly characterize glycosylated ectodomains across multiple coronavirus-family proteins. We assayed ∼200 variant spikes their expression, ACE2 binding, recognition by 13 nAbs. An alanine scan...
TnpB is an evolutionarily diverse family of RNA-guided endonucleases associated with prokaryotic transposons. Due to their small size and putative evolutionary relationship Cas12s, holds significant potential for genome editing mechanistic exploration. However, most TnpBs lack robust gene-editing activity, unbiased profiling mutational effects on activity has not been experimentally explored. Here, we mapped comprehensive sequence-function landscapes a ribonucleoprotein discovered many...
The ongoing evolution of SARS-CoV-2 into more easily transmissible and infectious variants has provided unprecedented insight mutations enabling immune escape. Understanding how these affect the dynamics antibody-antigen interactions is crucial to development broadly protective antibodies vaccines. Here we report characterization a potent neutralizing antibody (N3-1) identified from COVID-19 patient during first disease wave. Cryogenic electron microscopy revealed quaternary binding mode...
Abstract The SARS-CoV-2 spike (S) protein is a critical component of subunit vaccines and target for neutralizing antibodies. Spike also undergoing immunogenic selection with clinical variants that increase infectivity partially escape convalescent plasma. Here, we describe display, high-throughput platform to rapidly characterize glycosylated ectodomains across multiple coronavirus-family proteins. We assayed ∼200 variant spikes their expression, ACE2 binding, recognition by thirteen...
Abstract Mobile genetic elements evade CRISPR-Cas adaptive immunity by encoding anti-CRISPR proteins (Acrs). Acrs inactivate systems via diverse mechanisms but are generally specific for a narrow subset of Cas nucleases that share high sequence similarity. Here, we demonstrate AcrIIA11 inhibits Cas9 sub-types in vitro and human cells. Single-molecule fluorescence imaging reveals interferes with the first steps target search reducing S. aureus Cas9’s diffusion on non-specific DNA. DNA...