A5075371390- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Platelet Disorders and Treatments
- Chronic Myeloid Leukemia Treatments
- Acute Myeloid Leukemia Research
- Cell Adhesion Molecules Research
- Systemic Lupus Erythematosus Research
- Blood groups and transfusion
- T-cell and B-cell Immunology
- Hepatitis B Virus Studies
- Liver Disease Diagnosis and Treatment
- Bacteriophages and microbial interactions
- Cystic Fibrosis Research Advances
- Pancreatitis Pathology and Treatment
- Hepatitis C virus research
- Liver Disease and Transplantation
- Immune Cell Function and Interaction
- Pancreatic and Hepatic Oncology Research
- Lipid metabolism and disorders
- Blood properties and coagulation
- Kruppel-like factors research
- Probiotics and Fermented Foods
- Chronic Lymphocytic Leukemia Research
- Diabetes Treatment and Management
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Blood Coagulation and Thrombosis Mechanisms
Zhongnan Hospital of Wuhan University
2023-2024
Wuhan University
2023-2024
Capital Medical University
2009-2024
Beijing Chao-Yang Hospital
2009-2024
Second Hospital of Hebei Medical University
2023-2024
Hebei Medical University
2023-2024
Institute of Hematology & Blood Diseases Hospital
2013-2023
Chinese Academy of Medical Sciences & Peking Union Medical College
2013-2023
Shanghai Jiao Tong University
2016-2022
Shanxi Medical University
2018-2022
The F508del mutation impairs trafficking of the cystic fibrosis transmembrane conductance regulator (CFTR) to plasma membrane and results in a partially functional chloride channel that is retained endoplasmic reticulum degraded. We recently used novel high-throughput screening (HTS) assay identify small-molecule correctors CFTR found several classes hits screen 2000 compounds (Carlile et al., 2007). In present study, we have extended 42,000 confirmed sildenafil as corrector using this...
High-throughput small-molecule screens hold great promise for identifying compounds with potential therapeutic value in the treatment of protein-trafficking diseases such as cystic fibrosis (CF) and nephrogenic diabetes insipidus (NDI). The approach usually involves expressing mutant form gene cells assaying function a multiwell format when are exposed to libraries compounds. Although functional assays useful, they do not directly test ability compound correct defective trafficking protein....
Abstract Background and Aim: To profile changes of coagulation, anticoagulation fibrolytic factors associated with liver function failure portal vein thrombosis (PVT) formation in chronic cirrhosis patients. Methods: A total 116 cirrhotic patients admitted to our hospital from June 2006 October 2008 were included study. All classified into two groups: PVT group (31 patients), composed a control (85 including without PVT. Platelet, prothrombin time (PT), activated partial (APTT) fibrinogen...
The receptor for the type 1 insulin-like growth factor (IGF-I) regulates multiple cellular functions impacting on metastatic phenotype of tumor cells, including proliferation, anchorage-independent growth, survival, migration, synthesis 72-kDa IV collagenase and invasion. We have used site-directed mutagenesis to generate domain-specific mutants β subunit analyze role specific tyrosines in regulation invasive/metastatic phenotype. Poorly invasive M-27 carcinoma cells expressing low numbers...
In this study, traditional culture method and 16S rRNA gene analysis were applied to reveal the composition diversity of lactic acid bacteria (LAB) fermented cow milk, huruud urum from Baotou Bayannur midwestern Inner Mongolia. Also, quantitative results dominant LAB species in three different types dairy products gained by polymerase chain reaction (q-PCR) technology. Two hundred two strains isolated sixty-six samples identified classified into four genera, namely Enterococcus, Lactococcus,...
Summary Human umbilical cord matrix/Wharton's Jelly (hUC)-derived mesenchymal stem cells (MSC) have been shown to marked therapeutic effects in a number of inflammatory diseases and autoimmune humans based on their potential for immunosuppression low immunogenicity. Currently, no data are available the effectiveness UC-MSC transplantation immune thrombocytopenia (ITP) patients. It was objective this study assess effect allogeneic UC-MSCs ITP patients vitro vivo. Peripheral blood mononuclear...
Most cases of cystic fibrosis (CF) are caused by the deletion a single phenylalanine residue at position 508 transmembrane conductance regulator (CFTR). The mutant F508del-CFTR is retained in endoplasmic reticulum and degraded, but can be induced low temperature incubation (29°C) to traffic plasma membrane where it functions as chloride channel. Here we show that, cardiac glycosides, nanomolar concentrations, partially correct trafficking human CF bronchial epithelial cells (CFBE41o-) an...
Abstract Background Many genetic diseases are due to defects in protein trafficking where the mutant is recognized by quality control systems, retained endoplasmic reticulum (ER), and degraded proteasome. In many cases, retains function if it can be trafficked its proper cellular location. We have identified structurally diverse correctors that restore of most common mutation causing cystic fibrosis, F508del-CFTR. Most these do not act directly as ligands CFTR, but indirectly on other...
To investigate the value of D-dimer and protein S plasma concentrations for diagnosis portal vein thrombosis (PVT) in patients with liver cirrhosis.D-dimer were quantified, PVT was diagnosed by dynamic enhanced computed tomography function classified using Child-Pugh system. Receiver operating curve analysis performed.D-dimer increased, decreased, decreasing total study population (n = 188). significantly higher lower 51) than those without 137). had high specificity negative predictive...
Chronic immune thrombocytopenia (ITP) is characterized by autoimmune-mediated platelet destruction and impairment of thrombopoiesis. Mesenchymal stem cells (MSCs) are proposed to exhibit modulatory functions in self-tolerance maintenance. In this study, we aimed characterize phenotypically functionally bone marrow (BM)-derived MSCs from adult chronic ITP patients. Our results showed that BM-MSCs patients with exhibited impaired proliferation, abnormal morphology excessive apoptosis, these...
Background: Esophageal squamous cell carcinoma (ESCC) is the most prevalent histological type of esophageal cancer, but there a lack definite prognostic markers for this cancer. Methods: We used ESTIMATE algorithm to access tumor microenvironment (TME) ESCC cases deposited in TCGA database, and identified TME-related genes using Cox regression analysis. A least absolute shrinkage selector operation or LASSO was identify key genes. Risk scores were calculated, clinical predictive model...
The generation of antiviral drugs from herbs and other natural resources with traditionally long-confirmed effects is an efficient approach. So far, no herb or components that could inhibit bovine herpesvirus type 1 (BoHV-1) replication have been described. In this study, the effect curcumin, a phenolic constituent spice turmeric, on BoHV-1 was evaluated in cell culture. We demonstrated curcumin impairs viral particles affects virus post-binding entry process. Furthermore, upregulated...
Objective To assess changes in anticoagulation and fibrinolytic systems between biliary hyperlipidaemic acute pancreatitis (AP). Methods Patients with or AP were enrolled. Demographic clinical data collected, antithrombin III (ATIII), protein C, S, D-dimer levels investigated. Results A total of 45 patients 50 included (68 mild 27 moderately-severe AP). ATIII C the group significantly higher, but prothrombin time lower, versus group. found to be risk factors for AP. could predict severity,...
Abstract Objectives In patients with essential thrombocythemia ( ET ), vascular complications contribute to both morbidity and mortality. To better predict the occurrence of thrombotic events, an International Prognostic Score thrombosis for IPSET ‐thrombosis) was recently developed. We hereby presented external validation analysis this model in a large Cohort Chinese Patients. Methods retrospectively evaluated characteristics risk factors 970 estimated clinical implications ‐thrombosis...
Summary Primary immune thrombocytopenia ( ITP ) is an acquired autoimmune disease with many dysfunctions. Micro RNA s (mi s) are a class of non‐coding that post‐transcriptionally regulate gene expression by messenger degradation or translational repression. Accumulating evidence has demonstrated mi play vital role in the regulation immunological functions and prevention autoimmunity. However, whether involved pathogenesis still unknown. To illustrate , profile from peripheral blood...
Summary Primary immune thrombocytopenia ( ITP ) is an autoimmune disorder. Interleukin‐35 IL 35) can suppress T cell proliferation and elicit the development of inducible regulatory cells (Tregs). Previous studies have shown decreased plasma 35 levels dysfunctional in patients with . In this study, we determined whether correlate dysfunction patients. Plasma were found to be lower than healthy controls, positively correlated platelet percentage peripheral circulating Tregs, negatively...