A5029286210- Mosquito-borne diseases and control
- Malaria Research and Control
- Viral Infections and Vectors
- Insect symbiosis and bacterial influences
- Nitric Oxide and Endothelin Effects
- Sulfur Compounds in Biology
- HIV Research and Treatment
- HIV/AIDS drug development and treatment
- Neuroscience of respiration and sleep
- Insect Pest Control Strategies
- Liver physiology and pathology
- CRISPR and Genetic Engineering
- Viral Infections and Immunology Research
- RNA Interference and Gene Delivery
- Plant biochemistry and biosynthesis
- Natural product bioactivities and synthesis
- Cytomegalovirus and herpesvirus research
- Epigenetics and DNA Methylation
- Organ Transplantation Techniques and Outcomes
- Cannabis and Cannabinoid Research
- Viral Infections and Outbreaks Research
- Drug-Induced Hepatotoxicity and Protection
National University of Singapore
2013-2023
National University Health System
2016-2023
Ontario Institute for Cancer Research
1990-1994
University of Toronto
1990-1994
Dengue virus (DENV) is one of the most important arthropod-borne pathogens that cause life-threatening diseases in humans. However, no vaccine or specific antiviral available for dengue. As seen other RNA viruses, innate immune system plays a key role controlling DENV infection and disease outcome. Although interferon (IFN) response, which central to host protective immunity, has been reported limit replication, molecular details how modulated by IFN treatment are elusive. In this study,...
Curdlan sulfate (CRDS), a sulfated 1→3-β-D glucan, previously shown to be potent HIV entry inhibitor, is characterized in this study as inhibitor of the Dengue virus (DENV). CRDS was identified by silico blind docking studies exhibit binding potential envelope (E) protein DENV. inhibit DENV replication very efficiently different cells vitro. Minimal effective concentration low 0.1 µg/mL LLC-MK2 cells, and toxicity observed only at concentrations over 10 mg/mL. can also DENV-1, 3, 4...
Abstract The mosquito-borne Zika virus is an emerging pathogen from the Flavivirus genus for which there are no approved antivirals or vaccines. Using clinically validated PDK-53 dengue vaccine strain as a backbone, we created chimeric dengue/Zika virus, VacDZ, live attenuated candidate against virus. VacDZ demonstrates key markers of attenuation: small plaque phenotype, temperature sensitivity, attenuation neurovirulence in suckling mice, and pathogenicity interferon deficient adult AG129...
Dengue virus (DENV) is the cause of dengue fever, infecting 390 million people worldwide per year. It transmitted to humans through bites mosquitoes and could potentially develop severe symptoms. In spite rising social economic impact inflicted by disease on global population, a conspicuous lack efficacious therapeutics against DENV still persists. this study, catechin, natural polyphenol compound, was evaluated as infection inhibitor in vitro. Through time-course studies, catechin shown...
Integration, one of the hallmarks retrovirus replication, is mediated by a nucleoprotein complex called preintegration (PIC), in which viral DNA associated with many protein components that are required for completion early phase infection. A striking feature PIC its powerful integration activity vitro. The PICs from freshly isolated cytoplasmic extract infected cells able to insert into exogenously added target Therefore, PIC-based vitro assay reliable system assessing factors influencing...
Due to the nature of RNA viruses, their high mutation rates produce a population closely related but genetically diverse termed quasispecies. To determine role quasispecies in DENV disease severity, 22 isolates (10 from mild cases, 12 fatal cases) were obtained, amplified, and sequenced with Next Generation Sequencing using Illumina MiSeq platform. Using variation calling, unique wildtype nucleotide positions selected analyzed for variant nucleotides between cases. The analysis cases showed...
Virus genome recoding is an attenuation method that confers genetically stable by rewriting a virus with numerous silent mutations. Prior flavivirus attempts utilised codon deoptimisation approaches. However, these approaches act in species dependent manner and were unable to confer mosquito cells or animal models. To overcome limitations, we performed using the contrary approach of optimisation. The genomes flaviviruses such as dengue type 2 (DENV2) Zika (ZIKV) contain functional RNA...
Journal Article DNA methylation in specific cells of rat liver by N-nitrosodimethylamine and N-nitrosomethylbenzylamine Get access W.-D. Dai, Dai 2Present address: Department Pharmacology Medicine, Royal Hallampshire HospitalSheffield S10 2JF, UK Search for other works this author on: Oxford Academic PubMed Google Scholar V. Lee, Lee 1Department Medical Biophysics, University Toronto, Ontario Cancer Institute500 Sherbourne Street, Toronto M4X 1K9, Canada W. Chin, Chin D.P. Cooper, Cooper...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTEvidence that the hepatotoxicity of N-nitrosodimethylamine in rat is unrelated to DNA methylationWei Chin, Valentia M. Lee, and Michael C. ArcherCite this: Chem. Res. Toxicol. 1993, 6, 3, 372–375Publication Date (Print):May 1, 1993Publication History Published online1 May 2002Published inissue 1 1993https://pubs.acs.org/doi/10.1021/tx00033a019https://doi.org/10.1021/tx00033a019research-articleACS PublicationsRequest reuse permissionsArticle...