Gibberellins (GAs) are a class of plant hormones involved in the regulation flower development Arabidopsis . The GA-deficient ga1 - 3 mutant shows retarded growth all floral organs, especially abortive stamen that results complete male sterility. Until now, it has not been clear how GA regulates late-stage organs after establishment their identities within meristems. Various combinations null mutations DELLA proteins can gradually rescue defects In particular, synergistic effect rga t2 and...

Studies in the past have illuminated potential benefit of resveratrol as an anticancer (pro-apoptosis) and life-extending (pro-survival) compound. However, these two different effects were observed at concentration ranges. a wide range concentrations on same cell type are lacking, which is necessary to comprehend its diverse sometimes contradictory cellular effects. In this study, we examined self-renewal differentiation human mesenchymal stem cells (hMSCs), adult that reside number tissues,...

We have isolated a new mutant, hanaba taranu (han), which affects both flower and shoot apical meristem (SAM) development in Arabidopsis thaliana. Mutants fused sepals reduced organ numbers all four whorls, especially the 2nd (petal) 3rd (stamen) whorls. han meristems can become flatter or smaller than wild type. HAN encodes GATA-3-like transcription factor with single zinc finger domain. is transcribed at boundaries between its newly initiated primordia different floral It also expressed...

Mitotic clonal expansion has been suggested as a prerequisite for adipogenesis in murine preadipocytes, but the precise role of cell proliferation during human is unclear. Using adipose tissue-derived mesenchymal stem cells an vitro model adipogenic study, group cycle regulators, including Cdk1 and CCND1, were found to be downregulated early 24 h after initiation consistently, activity was restricted first 48 induction. Cell either further inhibited using siRNAs targeting genes or enhanced...

To better define Abd-B type homeodomain function, to test models that predict functional equivalence of all Hox genes and initiate a search for the downstream targets Hoxa13, we have performed homeobox swap by replacing Hoxa11 gene with theHoxa13 gene. The Hoxa13 are contiguous located at 5′ end HoxA cluster. modified allele (A1113hd)showed near wild-type function in development kidneys, axial skeleton male reproductive tract, consistent models. In limbs female however, theA1113hd appeared...

Tissue-specific (or adult) stem/progenitor cells are regarded as the source for normal tissue homeostasis and repair. They also provide tremendous promise regenerative medicine because of their capacity to proliferate differentiate into a variety mature cell types. Human mesenchymal stem (hMSCs) can osteocytes, adipocytes, chondrocytes, muscle cells, neurons. However, molecular mechanisms underlying these differentiation processes poorly understood. We screened synthetic siRNA library...

Fagus longipetiolata Seemen is a deciduous tree of the genus in Fagaceae, which endemic to China. In this study, we successfully sequenced cp genome F. longipetiolata, compared genomes genus, and reconstructed phylogeny Fagaceae. The results showed that was 158,350 bp, including pair inverted repeat (IRA IRB) regions with length 25,894 bp each, large single-copy (LSC) region 87,671 small (SSC) 18,891 bp. encoded 131 unique genes, 81 protein-coding 37 transfer RNA genes (tRNAs), 8 ribosomal...

Epigenetic regulation is a critical component of lineage determination. Adipogenesis the process through which uncommitted stem cells or adipogenic precursor differentiate into adipocytes, most abundant cell type adipose tissue. Studies examining chromatin modification during adipogenesis have provided further understanding molecular blueprint that controls onset differentiation. Unlike histone acetylation, methylation has context dependent effects on activity transcribed region DNA, with...

Hematopoietic stem cells recipients remain susceptible to opportunistic viral infections including herpes simplex virus type‐1 (HSV‐1). The purpose of this investigation was analyze susceptibility human mesenchymal (hMSCs) HSV‐1 infection and identify the major entry receptor. Productive in hMSCs confirmed by replication plaque formation assays using a syncytial KOS (804) virus. To examine significance receptors, RT‐PCR antibody‐blocking were performed. data showed expression gD receptors:...

Understanding the molecular basis underlying formation of bone-forming osteocytes and lipid-storing adipocytes will help provide insights into cause disorders originating in stem/progenitor cells develop therapeutic treatments for bone- or adipose-related diseases. In this study, role RGS2 RGS4, two members regulators G protein signaling (RGS) family, was investigated during adipogenenic osteogenenic differentiation human mesenchymal stem (hMSCs).Expression RGS4 were found to be inversely...

Many different culture systems have been developed for expanding human pluripotent stem cells (hESCs and hiPSCs). In general, 4–10 ng/ml of bFGF is supplemented in media feeder-dependent regardless feeder cell types, whereas feeder-free systems, up to 100 required maintaining long-term on various substrates. The amount native hESCs growth niche unclear. Here we report using inactivated adipose-derived mesenchymal as examine parallel cultures two lines (H1 H9) one hiPSCs line (DF19-9-7T) with...

Thermal insulation materials typically used in low-temperature applications such as pipelines for transporting liquid natural gas can become brittle and are therefore susceptible to impact damage. New feather-fibre biomaterials developed this work have extremely low thermal conductivity but retain high resistance at low-temperatures. The experiments reported demonstrate the improved of feather fibre compared foamed nitrile rubber. microstructural characteristics that allow them be resistant...

Pinus massoniana Lamb. is an evergreen conifer; however, some current-year seedlings exhibit “winter reddening” at the onset of winter. The biological significance this reddening unclear. We examine physiological responses needles during process and explore relationships between traits seeding cold resistance. Based on needle color, we recognize non-reddened, partially reddened, fully reddened stages. As progresses, chlorophyll fluorescence parameters (maximum light energy conversion...

Human adipogenesis is the process through which uncommitted human mesenchymal stem cells (hMSCs) differentiate into adipocytes. Through a

The symbiosis between ectomycorrhizal (ECM) fungal species and the root system of Pinus massoniana is important for its growth. However, characteristics ECM communities their relationships with extracellular enzyme activities nutrients in rhizosphere soil different P. genotypes have not been well characterized. In this study, families (groups offspring from parents) levels growth were selected investigating communities, nutrients, activity, leaf nutrient concentrations to explore composition...