A5013956070- Glycosylation and Glycoproteins Research
- Bacteriophages and microbial interactions
- Oral microbiology and periodontitis research
- Genomics and Phylogenetic Studies
- Carbohydrate Chemistry and Synthesis
- Streptococcal Infections and Treatments
- Probiotics and Fermented Foods
- Enzyme Production and Characterization
- Bacterial Genetics and Biotechnology
- HIV/AIDS oral health manifestations
- Enzyme Structure and Function
- Salivary Gland Disorders and Functions
- Monoclonal and Polyclonal Antibodies Research
- RNA and protein synthesis mechanisms
- Microbial Metabolites in Food Biotechnology
- Urban Agriculture and Sustainability
- Microbial Community Ecology and Physiology
- Force Microscopy Techniques and Applications
- Dental Health and Care Utilization
- Infective Endocarditis Diagnosis and Management
- Biochemical and Structural Characterization
- Land Use and Ecosystem Services
- Photosynthetic Processes and Mechanisms
- Mass Spectrometry Techniques and Applications
- Biochemical and Molecular Research
BOKU University
2016-2025
University of Vienna
1995-2024
Swedish University of Agricultural Sciences
2023
Wienerberger (Czechia)
2022
ETH Zurich
2022
Vienna Biocenter
2016-2021
Stockholm University
2018-2020
University of Wisconsin–Madison
2009
CIC biomaGUNE
2009
Gregor Mendel Institute of Molecular Plant Biology
2005-2008
Streptococci are primary colonizers of the oral cavity where they ubiquitously present and an integral part commensal biofilm microflora. The role streptococci play in interaction with host is ambivalent. On one hand, function as gatekeepers homeostasis a prerequisite for maintenance health - shape microbiota, modulate immune system to enable bacterial survival, antagonize pathogenic species. other also recognized pathogens, such Streptococcus mutans sobrinus , which trigger onset dental...
Tannerella forsythia , a member of the “red complex” bacteria implicated in severe periodontitis, employs various survival strategies and virulence factors to interact with host. It thrives as late colonizer oral biofilm, relying on its unique adaptation mechanisms for persistence. Essential are type 9 protein secretion system O -glycosylation proteins, crucial host interaction immune evasion. Virulence T. including sialidase proteases, facilitate pathogenicity by degrading glycoproteins...
Mutanofactins are a family of natural products produced by Streptococcus mutans from the human oral microbiome. We report unified approach to all mutanofactins developing total synthesis amenable diversification. The key success for most complex members, 607 and 697, was an acyl ketene based strategy. Access enabled comprehensive biological profiling, where we demonstrate that biofilm promoting in mutans. Experiments were extended other inhabitants microbiome first time: gordonii oralis, two...
The periodontal pathogen Tannerella forsythia possesses a glycosylated S-layer as an outermost cell decoration. While the provides selection advantage to bacterium in natural habitat, its virulence potential remains be investigated. In present study, immune responses of human macrophages and gingival fibroblasts upon stimulation with wild-type T. S-layer-deficient mutant were mRNA expression levels pro-inflammatory mediators IL-1β, TNF-α, IL-8 analyzed by qPCR, production corresponding...
The Gram-positive, spore-forming bacterium Paenibacillus larvae is the etiological agent of American Foulbrood (AFB), a globally occurring, deathly epizootic honey bee brood. AFB outbreaks are predominantly caused by two genotypes P. larvae, ERIC I and II, with II being more virulent genotype on larval level. Recently, comparative proteome analyses have revealed that but not might harbour functional S-layer protein, named SplA. We here determine genomic sequence splA in both demonstrate...
Summary Recently, the Surface (S)‐layer glycoprotein of thermoacidophilic crenarchaeote Sulfolobus acidocaldarius was found to be N ‐glycosylated with a heterogeneous family glycans, largest having composition Glc 1 Man 2 GlcNAc plus 6‐sulfoquinovose. However, genetic analyses genes involved in ‐glycosylation process Crenarchaeota were missing so far. In this study we identify gene cluster biosynthesis sulfoquinovose and important for assembly S‐layer ‐glycans. A successful markerless...
Glycosylation is a frequent and heterogeneous posttranslational protein modification occurring in all domains of life. While N-glycosylation at asparagine O-glycosylation serine, threonine or hydroxyproline residues have been studied great detail, only few data are available on O-glycosidic attachment glycans to the amino acid tyrosine. In this study, we describe identification characterization bacterial tyrosine system. Gram-positive, mesophilic bacterium Paenibacillus alvei CCM 2051T,...
The human gut symbiont Bacteroides fragilis has a general protein O-glycosylation system in which numerous extracytoplasmic proteins are glycosylated at three amino acid motif. In B. fragilis, glycosylation is fundamental and essential property as mutants with defects have impaired growth unable to competitively colonize the mammalian intestine. this study, we analysed phenotype of defective found that glycan added comprised core an outer glycan. genetic region encoding for synthesis...
Tannerella forsythia is the only 'red-complex' bacterium covered by an S-layer, which has been shown to affect virulence. Here, outer membrane vesicles (OMVs) enriched with putative glycoproteins are described as a new addition virulence repertoire of T. forsythia. Investigations this hampered its fastidious growth requirements and recently discovered mismatch available genome sequence (92A2 = ATCC BAA-2717) widely used strain (ATCC 43037). was grown anaerobically in serum-free medium...
Summary Conserved C‐terminal domains ( CTD ) have been shown to act as a signal for the translocation of certain proteins across outer membrane Bacteroidetes via type IX secretion system (T9 SS ). The genome sequence periodontal pathogen T annerella forsythia predicts presence components T9 in conjunction with suite proteins. . is covered two‐dimensional crystalline surface (S‐) layer composed glycosylated TfsA and TfsB. To investigate, if functional , ‐deficient mutants were generated by...
With increased urbanization, ecological challenges such as climate change and loss of biodiversity, stress-related disorders globally posing a major threat to public health wellbeing, the development efficient multiple-use strategies for urban green spaces infrastructures is great importance. In addition benefits water regulation, food production, biodiversity conservation, features have been associated with various wellbeing outcomes from psychological perspective. Research suggests links...
Mutanobactin D is a non-ribosomal, cyclic peptide isolated from Streptococcus mutans and shows activity reducing yeast-to-hyphae transition as well biofilm formation of the pathogenic yeast Candida albicans. We report first total synthesis this natural product, which relies on enantioselective, zinc-mediated 1,3-dipolar cycloaddition sequence cascading reactions, providing key lipidated γ-amino acid found in mutanobactin D. The enables configurational assignment, determination dominant...
<i>Geobacillus stearothermophilus</i> NRS 2004/3a possesses an oblique surface layer (S-layer) composed of glycoprotein subunits as the outermost component its cell wall. In addition to elucidation complete S-layer glycan primary structure and determination glycosylation sites, structural gene <i>sgsE</i> encoding protein was isolated by polymerase chain reaction-based techniques. The open reading frame codes for a 903 amino acids, including leader sequence 30 acids. mature has calculated...
Two moderately thermophilic, Gram-positive, spore-forming bacteria were isolated from different geographical locations and sources; strain GS5-97 T a beet sugar factory in Leopoldsdorf, Lower Austria, YNP10 geothermally heated soil, Yellowstone National Park, USA. The sequences of their 16S rRNA genes found to be 99·8 % identical, DNA–DNA hybridization experiments revealed that strains share 89·9 mol% similarity each other, but only 34·3 39·2 similarity, respectively, Geobacillus...