A5071758913- Innovations in Medical Education
- Glycosylation and Glycoproteins Research
- Dental Education, Practice, Research
- Medical Education and Admissions
- Probiotics and Fermented Foods
- Transgenic Plants and Applications
- Carbohydrate Chemistry and Synthesis
- Viral Infectious Diseases and Gene Expression in Insects
- Mycobacterium research and diagnosis
- European Criminal Justice and Data Protection
- Medical and Health Sciences Research
- RNA and protein synthesis mechanisms
- Global Health Workforce Issues
- Medical Practices and Rehabilitation
- European and International Law Studies
- Neuroinflammation and Neurodegeneration Mechanisms
- Ruminant Nutrition and Digestive Physiology
- Toxin Mechanisms and Immunotoxins
- Criminal Law and Evidence
- Air Quality and Health Impacts
- Reproductive Health and Technologies
- Proteoglycans and glycosaminoglycans research
- Infectious Diseases and Mycology
- Clinical Reasoning and Diagnostic Skills
- Oral microbiology and periodontitis research
North Carolina State University
2024-2025
Otto-von-Guericke University Magdeburg
2023
BOKU University
2011-2021
Milken Institute
2019-2021
George Washington University
2018-2021
University of Tübingen
1969-2021
Hertie Institute for Clinical Brain Research
2021
FH Campus Wien
2021
University of Florida
2011-2018
Florida College
2018
Abstract This article was developed for the Commission on Change and Innovation in Dental Education (CCI), established by American Association. CCI created because numerous organizations within organized dentistry educational community have initiated studies or proposed modifications to process of dental education, often working achieve positive desirable goals but without coordination communication. The fundamental mission is serve as a focal meeting place where educators administrators,...
Abstract In this article, the Task Force on Student Outcomes Assessment of American Dental Education Association's Commission Change and Innovation in describes current status student outcomes assessment U.S. dental education. This review is divided into six sections. The first summarizes literature students’ performance. Section two discusses catalysts, with a focus problem‐based learning, for development new methods, while third section presents several resources guides that can be used to...
A number of recent studies have shown the importance mammalian gut microbiome in host health. In context endangered species, a few examined relationship between wild versus captive populations due to digestive and other health issues. Unfortunately, results seem vary across taxa terms animals having higher, lower, or equivalent diversity relative their counterparts. Here, we focus on black rhinoceros as suffer from potentially dietary related effects. We compared microbiomes rhinos test for...
This article introduces a series of white papers developed by the ADEA Commission on Change and Innovation (CCI) to explore case for change in dental education. preamble argues that there is compelling need rethinking approach education United States. Three issues facing are explored: 1) challenging financial environment higher education, making schools very expensive tuition-intensive universities operate producing high debt levels students limit access restrict career choices; 2)...
Hyperglycosylated proteins are more stable, show increased serum half-life and less sensitivity to proteolysis compared non-sialylated forms. This applies particularly recombinant human erythropoietin (rhEPO). Recent progress in N-glycoengineering of non-mammalian expression hosts resulted vivo protein sialylation at great homogeneity. However the synthesis multi-sialylated N-glycans is so far restricted mammalian cells. Here we used a plant based system accomplish multi-antennary...
Summary Conserved C‐terminal domains ( CTD ) have been shown to act as a signal for the translocation of certain proteins across outer membrane Bacteroidetes via type IX secretion system (T9 SS ). The genome sequence periodontal pathogen T annerella forsythia predicts presence components T9 in conjunction with suite proteins. . is covered two‐dimensional crystalline surface (S‐) layer composed glycosylated TfsA and TfsB. To investigate, if functional , ‐deficient mutants were generated by...
Human butyrylcholinesterase (BChE) is considered a candidate bioscavenger of nerve agents for use in pre- and post-exposure treatment. However, the presence functional necessity complex N-glycans (i.e. sialylated structures) challenging issue respect to its recombinant expression. Here we transiently co-expressed BChE cDNA model plant Nicotiana benthamiana with vectors carrying genes necessary planta protein sialylation. Site-specific sugar profiling secreted (rBChE) collected from...
The importance of protein glycosylation in the biomedical field requires methods that not only quantitate structures by their monosaccharide composition, but also resolve and identify many isomers expressed mammalian cells. art unambiguous identification isomeric complex mixtures, however, did yet catch up with fast pace advance high-throughput glycomics. Here, we present a strategy for deducing help deci-minute accurate retention time library porous graphitic carbon chromatography mass...
Research is needed to understand the presence of SARS-CoV-2 virus in semen, sexual transmissibility, and impact on sperm quality. Several studies have examined men recovering from COVID-19, but large-scale community-based testing ascertain effects male reproductive tract, potential for prolonged transmission.
Ponazuril (Marquis®) is a triazine based antiprotozoal medication labeled to treat equine protozoal myeloencephalitis in the United States. often used an extra-label manner coccidiosis piglets, but tissue residue data limited. In this study, piglets were given single oral dose of 5 mg/kg ponazuril. Piglets (n=5) euthanized at eleven timepoints (0, 15, 28, 43, 57, 71, 85, 99, 113, 127, and 141 days) with sample collection chromatographic analysis. The maximum limit (MRL) values established by...
While it is now evident that the two Bacteroidales species Bacteroides fragilis and Tannerella forsythia both have general O-glycosylation systems share a common glycosylation sequon, ability of these organisms to glycosylate protein native other organism has not yet been demonstrated. Here, we report on heterologous proteins between organisms. Using genetic tools previously developed for species, B. model glycoproteins were expressed in fastidious anaerobe T. attachment known O-glycan was...
The cell surface of the oral pathogen Tannerella forsythia is heavily glycosylated with a unique, complex decasaccharide that O-glycosidically linked to bacterium's abundant (S-) layer, as well other proteins. S-layer glycoproteins are virulence factors T. and there evidence protein O-glycosylation underpins pathogenicity. To elucidate pathway, genes suspected encoding pathway components were first identified in genome sequence ATCC 43037 type strain, revealing 27-kb gene cluster was shown...
In order to preserve the in vivo metabolite levels of cells, a quenching protocol must be quickly executed avoid degradation labile metabolites either chemically or biologically. case mammalian cell cultures cultivated complex media, wash step previous is necessary contamination pellet with extracellular metabolites, which could distort real intracellular concentration metabolites. This typically achieved by one multiple centrifugation/wash steps delay time until (even harsh centrifugation...
Diffuse gliomas are the most common malignant tumors of central nervous system with poor treatment efficacy. Infiltration immune cells into during immunosurveillance is observed in multiple tumor entities and often associated a favorable outcome. The aim this study was to evaluate infiltration their association cerebrospinal fluid (CSF) cytokine concentrations.We applied immunohistochemistry tissue sections 18 high-grade glioma (HGG) patients (4 anaplastic astrocytoma, IDH-wildtype WHO-III;...
Per- and polyfluoroalkyl substances (PFAS) are found in a variety of places including cosmetics, rain jackets, dust, water. PFAS have also been applied to occupational gear protect against water oils. However, identified as immunosuppressants perfluorooctanoic acid (PFOA), specific PFAS, has carcinogenic. Since there is risk for dermal exposure these compounds, need characterize their absorption. Using vitro flow-through diffusion, skin permeabilities were determined 14C-labeled...
In all eukaryotes N-glycosylation is the most prevalent protein modification of secretory and membrane proteins. Although capacity individual steps N-glycan processing pathway have been well studied in model plant Arabidopsis thaliana, little attention has paid to characterization glycosylation status We report here structural analysis N-glycans present on endogenous thioglucoside glucohydrolases (myrosinases) TGG1 TGG2 from A. thaliana. All nine sites four are occupied by oligomannosidic...
Abstract The chloroplast genome of land plants contains only a single gene for splicing factor, Maturase K (MatK). To better understand the regulation matK expression, we quantitatively investigated expression across tobacco (Nicotiana tabacum) development at transcriptional, posttranscriptional, and protein levels. We observed striking discrepancies MatK messenger RNA levels in young tissue, suggestive translational or altered stability. furthermore found increased stability mature while...
The aim of this report is to provide guidance assist in the international convergence quality assurance, benchmarking and assessment systems improve dental education. Proposals are developed for mutual recognition qualifications, aid movement exchange staff students including supporting developing countries. Quality assurance responsibility all involved education involves three levels: internal, institutional external. Benchmarking information provides a subject framework. Benchmarks useful...
With no acceptable method for collecting fresh rumen fluid from zoo ruminants, it was proposed that fecal bacterial concentrations may be correlated with bacteria. If so, could used to study both the effects of diet on bacteria as well abnormalities. Total and cellulolytic were determined in whole contents feces sheep using a most-probable-number (MPN) assay. In Latin square design, four crossbred ewes fed diets 100% long or chopped orchardgrass hay (OH) 60% ground shelled corn plus 40% OH....