A5037406946- Sphingolipid Metabolism and Signaling
- Lipid Membrane Structure and Behavior
- Endoplasmic Reticulum Stress and Disease
- Lysosomal Storage Disorders Research
- Carbohydrate Chemistry and Synthesis
- Liver physiology and pathology
- Cannabis and Cannabinoid Research
- Biochemical and Molecular Research
- Andrographolide Research and Applications
- Drug-Induced Hepatotoxicity and Protection
- Natural Antidiabetic Agents Studies
- Cellular transport and secretion
- Carcinogens and Genotoxicity Assessment
- Biofuel production and bioconversion
- Genomics, phytochemicals, and oxidative stress
- Liver Disease Diagnosis and Treatment
- Polyamine Metabolism and Applications
- Microbial Metabolic Engineering and Bioproduction
- Genetic and Kidney Cyst Diseases
- Erythrocyte Function and Pathophysiology
- Metabolism, Diabetes, and Cancer
- Genetic Syndromes and Imprinting
- Toxic Organic Pollutants Impact
- Calcium signaling and nucleotide metabolism
- Biochemical effects in animals
Connexios (India)
2013-2015
National Cancer Institute
2009-2014
SDM College of Medical Science and Hospital
2014
Shilpa (India)
2013
Toxicologie, Pharmacologie et Signalisation Cellulaire
2009
National Institutes of Health
2007
Indian Institute of Science Bangalore
2004-2006
Sphingolipids, a family of membrane lipids, are bioactive molecules that participate in diverse functions controlling fundamental cellular processes such as cell division, differentiation, and death. Given most these form the basis for several pathologies, it is not surprising sphingolipids key players pathological processes. This review discusses role sphingolipid metabolic pathway diabetes, Alzheimer’s disease, hepatocellular carcinoma, with special emphasis on changes gene expression...
Ceramide transfer protein (CERT) transfers ceramide from the endoplasmic reticulum to Golgi complex, a process critical in synthesis and maintenance of normal levels sphingolipids mammalian cells. However, how its function is integrated into development physiology animal less clear. Here, we report vivo consequences loss functional CERT protein. We generated Drosophila melanogaster mutant flies lacking (Dcert) using chemical mutagenesis Western blot-based genetic screen. The die early...
Ceramide transfer protein (CERT) functions in the of ceramide from endoplasmic reticulum (ER) to Golgi. In this study, we show that CERT is an essential gene for mouse development and embryonic survival and, quite strikingly, critical mitochondrial integrity. mutant embryos accumulate ER but also mislocalize mitochondria, compromising their function. Cells abnormal dilation degenerating mitochondria. These subcellular changes manifest as heart defects cause severely compromised cardiac...
Phosphoinositide-specific phospholipase C (PLC) is a central effector for many biological responses regulated by G-protein–coupled receptors including Drosophila phototransduction where light sensitive channels are activated downstream of NORPA, PLCβ homolog. Here we show that the sphingolipid biosynthetic enzyme, ceramide kinase, novel regulator PLC signaling and photoreceptor homeostasis. A mutation in kinase specifically leads to proteolysis consequent loss activity, failure signal...
Ceramide transfer protein (CERT) transfers ceramide from the endoplasmic reticulum (ER) to Golgi complex. Its deficiency in mouse leads embryonic death at E11.5. CERT deficient embryos die cardiac failure due defective organogenesis, but not induced apoptotic or necrotic cell death. In current study we examined effect of a primary line, namely, fibroblasts (MEFs). We show that MEFs, unlike mutant embryos, lack does lead increased causes an accumulation hexosylceramides. Nevertheless, defects...
Precision-cut liver slices present different cell types of in a physiological context, and they have been explored as effective vitro model systems to study fibrosis. Inducing fibrosis the using toxicants like carbon tetrachloride is less relevance human disease conditions. Our aim for this was establish physiologically relevant conditions induce fibrotic phenotypes slices. 150 μm thickness were obtained from female C57BL/6 J mice. The cultured 24 hours media containing cocktail 10 nM each...
Ceramidases catalyze the conversion of ceramide to sphingosine. They are acylaminohydrolases that deacylation amide-linked saturated fatty acid from generate also reverse reaction biosynthesis using sphingosine and acid. In mammals, different proteins these reactions while individually exhibiting optimal activity over a narrow pH range have been accordingly called acid, neutral, alkaline ceramidases. Several genes encode for variants ceramidase in mammals. Brainwashing (Bwa) is only putative...
Sphingolipids are an important class of compounds that regulate signal transduction and other vital cellular processes. Herein, we report sensitive normal reversed phase LC-MS/MS methods for quantitation multiple sphingolipid classes. In the normal-phase ESI/MS/MS method, a high content organic solvents was utilized, which, although it included hexane, ethyl acetate, acetonitrile containing 2% methanol, 1-2% acetic acid, 5 mM ammonium resulted in very efficient electrospray ionization...
AMP activated protein kinase (AMPK) regulates key metabolic reactions and plays a major role in glucose homeostasis. Activating the AMPK is considered as one of potential therapeutic strategies treating type-2 diabetes. However, targeting by small molecule mediated approach can be challenging owing to diverse isoforms enzyme their varied combination different tissues. In current study we employ novel strategy achieving activation through increasing levels cellular (an allosteric activator...
Sir, Bardet-Biedl syndrome (BBS) is a rare autosomal recessive disorder with pan systemic manifestations characterized principally by obesity, cone-rod dystrophy, postaxial polydactyly, cognitive impairment, male hypogonadotrophic hypogonadism, complex female genitourinary malformations and renal dysfunction.[12] Incidence varies from 1:13,500 in the Middle East to 1:160,000 rest of world. We report anesthetic management patient this very scheduled for an elective surgery. An 8-year-old boy...