Autographa californica nuclear polyhedrosis virus (AcNPV) was used as an expression vector for human beta interferon. By using specially constructed plasmids, the protein-coding sequences interferon were linked to AcNPV promoter gene encoding polyhedrin, major occlusion protein. The inserted at various locations relative polyhedrin transcriptional and translational signals, interferon-polyhedrin hybrid genes transferred infectious vectors. Biologically active produced, greater than 95%...

The development of a spider silk-manufacturing process is great interest. However, there are serious problems with natural manufacturing through farming, and standard recombinant protein production platforms have provided limited progress due to their inability assemble silk proteins into fibers. Thus, we used piggyBac vectors create transgenic silkworms encoding chimeric silkworm/spider proteins. fibers produced by these animals were composite materials that included integrated in an...

Transposon mutagenesis of baculoviruses provides an ideal experimental system for analysis the movement a unique family mobile element identified from lepidopteran genomes. Members this short-inverted-repeat elements are characterized by their extreme specificity TTAA target sites. This report describes excision events two representatives family, tagalong (formerly TFP3) and piggyBac IFP2). These were tagged with polyhedrin/lacZ reporter gene inserted back into virus genome either homologous...

Mutants of Autographa californica and Galleria mellonella nuclear polyhedrosis viruses, which produce an altered plaque phenotype as a result reduced numbers viral occlusions in infected cells, were isolated after passage Trichoplusia ni (TN-368) cells. These mutants, termed FP (few-polyhedra) had acquired cell DNA sequences ranging from 0.8 to 2.8 kilobase pairs size. The insertions occurred specific region between 35.0 37.7 map units the A. genome. A cloned fragment containing one host...

We describe a method to introduce site-specific mutations into the genome of Autographa californica nuclear polyhedrosis virus. Specifically, A. virus gene for polyhedrin, major protein that forms viral occlusions in infected cells, was mutagenized by introducing deletions cloned DNA fragment containing gene. The polyhedrin transferred intact mixing and DNAs, cotransfecting Spodoptera frugiperda screening recombinants had undergone allelic exchange. Recombinant viruses with mutant genes were...

The piggyBac (IFP2) short inverted terminal repeat transposable element from the cabbage looper Trichoplusia ni was tested for gene transfer vector function as part of a bipartite vector–helper system in Mediterranean fruit fly Ceratitis capitata . A marked with medfly white normally regulated transposase helper at two different concentrations eye host strain. Both experiments yielded transformants an approximate frequency 3–5%, total six lines isolated having pigmented eyes various levels...

Mutagenesis of baculoviruses by host mobile elements occurs spontaneously and frequently during propagation the viruses in Lepidopteran cell cultures. Most transposons identified as insertions baculovirus genomes are relatively small Class II that exhibit a remarkable specificity for TTAA target sites. We have developed transposition assay to analyze movement these TTAA-specific using genome lacZ gene under control polyhedrin promoter selective marker transposon. This provides first...

Functional analysis of the Plasmodium falciparum genome is restricted because limited ability to genetically manipulate this important human pathogen. We have developed an efficient transposon-mediated insertional mutagenesis method much needed for high-throughput functional genomics malaria parasites. A drug-selectable marker, dihydrofolate reductase, added lepidopteran transposon piggyBac , transformed parasites by integration into P. in presence a transposase-expressing helper plasmid....

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTOligosaccharide processing in the expression of human plasminogen cDNA by lepidopteran insect (Spodoptera frugiperda) cellsDonald J. Davidson, Malcolm Fraser, and Francis CastellinoCite this: Biochemistry 1990, 29, 23, 5584–5590Publication Date (Print):June 12, 1990Publication History Published online1 May 2002Published inissue 12 June 1990https://pubs.acs.org/doi/10.1021/bi00475a024https://doi.org/10.1021/bi00475a024research-articleACS...

The assembly and maturation of Autographa californica nuclear polyhedrosis virus virions was observed in detail. Nucleocapsid appeared to begin with the capsid sheaths on base structures pockets virogenic stroma. partially assembled precapsid associate end-on matrix stroma during acquisition nucleoprotein core complex. Packaging complex be associated cap structure. length measurements between wild-type insertion-mutant revealed that greater genome size leads increased length. A model...

Autographa californica nuclear polyhedrosis virus (AcNPV) was used as an expression vector for human beta interferon. By using specially constructed plasmids, the protein-coding sequences interferon were linked to AcNPV promoter gene encoding polyhedrin, major occlusion protein. The inserted at various locations relative polyhedrin transcriptional and translational signals, interferon-polyhedrin hybrid genes transferred infectious vectors. Biologically active produced, greater than 95%...

Abstract A previously reported piggyBac minimal sequence cartridge, which is capable of efficient transposition in embryo interplasmid assays, failed to produce transformants at a significant frequency Drosophila melanogaster compared with full‐length or less extensive internal deletion constructs. We have re‐examined the importance these domain (ID) sequences for germline transformation using PCR strategy that effectively adds increasing lengths ID each terminus. series synthetic plasmids...

The transposon piggyBac from the genome of cabbage looper moth Trichoplusia ni has been observed in laboratory to jump into genomes key model and pathogenic eukaryote organisms including mosquitoes, planarians, human other mammalian cells, malaria parasite Plasmodium falciparum. Introduction exogenous transposons schistosomes not reported but transposon-mediated transgenesis might supersede current methods for functional genomics this important pathogen. In present study we examined whether...

Much of the Plasmodium falciparum genome encodes hypothetical proteins with limited homology to other organisms. A lack robust tools for genetic manipulation parasite limits functional analysis these and aspects genome. Transposon mutagenesis has been used widely identify gene functions in many organisms would be extremely valuable genome.In this study, we investigated lepidopteran transposon, piggyBac, as a molecular tool characterization Through multiple transfections, generated 177 unique...

Pmel 17 is preferentially expressed in pigment cells a manner suggestive of involvement melanin biosynthesis. The gene identical to the silver (si) pigmentation locus mice. We now produced recombinant glutathione-S-transferase-human infusion protein and raised polyclonal antibodies against it confirm ultrastructural location presumed site action predicted by deduced primary structure 17/silver, authenticate specificity DHICA converting function as inherent silver-locus protein. Full-length...