A5065772874- Plant Molecular Biology Research
- Plant nutrient uptake and metabolism
- Plant Reproductive Biology
- Single-cell and spatial transcriptomics
- Polysaccharides and Plant Cell Walls
- Kruppel-like factors research
- CRISPR and Genetic Engineering
- Cancer-related molecular mechanisms research
- Photosynthetic Processes and Mechanisms
- Advanced Fluorescence Microscopy Techniques
- Cell Image Analysis Techniques
- Plant Virus Research Studies
University of Helsinki
2019-2025
Abstract Vascular cambium contains bifacial stem cells, which produce secondary xylem to one side and phloem the other. However, how these fate decisions are regulated is unknown. Here we show that positioning of an auxin signalling maximum within determines cell daughters. The position modulated by gibberellin-regulated, PIN1-dependent polar transport. Gibberellin treatment broadens from towards phloem. As a result, xylem-side daughter preferentially differentiates into xylem, while...
During primary growth, plant tissues increase their length, and as these mature, they initiate secondary growth to thickness.1 It is not known what activates this transition growth. Cytokinins are key hormones regulating vascular development during both of Arabidopsis roots, cytokinins promote procambial cell proliferation2,3 patterning together with the hormone auxin.4-7 In absence cytokinins, fails initiate.8 Enhanced cytokinin levels, in turn, growth.8,9 Despite importance little about...
Abstract During secondary growth, the vascular cambium produces conductive xylem and phloem cells, while phellogen (cork cambium) deposits phellem (cork) as outermost protective barrier. Although most of tissues are made up parenchyma which also produced by both cambia, their diversity function poorly understood. Here we combined single-cell RNA sequencing analysis with lineage tracing to recreate developmental trajectories cell types in Arabidopsis root undergoing growth. By analysing 93...
Abstract During the process of secondary growth, vascular cambium produces conductive xylem and phloem cells, while phellogen (cork cambium) deposit phellem (cork) as outermost protective barrier. Although most tissues is made up by parenchyma cells which are also produced both cambia, their diversity function poorly understood. Here we combined single-cell RNA sequencing analysis with lineage tracing to recreate developmental trajectories cell types in Arabidopsis root undergoing growth. By...
Abstract Aims Recent advancements in single‐cell transcriptomics have facilitated the possibility of acquiring vast amounts data at resolution. This development has provided a broader and more comprehensive understanding complex biological processes. The growing datasets require visualization tool that transforms into an intuitive representation. To address this challenge, we utilized open‐source 3D software Blender to design Cella, cell atlas tool, which heatmaps can be rendered image...
Abstract Vascular cambium contains bifacial stem cells, which produce secondary xylem to one side and phloem the other. However, how these fate decisions are regulated is unknown. Here, we show that positioning of an auxin signalling maximum within determines cell daughters. The position modulated by gibberellin-regulated, PIN1-dependent polar transport. Gibberellin treatment broadens from towards phloem. As a result, xylem-side daughter preferentially differentiates into xylem, while...