Shelly Kalaora
A5066319447- Immunotherapy and Immune Responses
- vaccines and immunoinformatics approaches
- CAR-T cell therapy research
- Cancer Immunotherapy and Biomarkers
- Cell Image Analysis Techniques
- Advanced Biosensing Techniques and Applications
- Melanoma and MAPK Pathways
- Single-cell and spatial transcriptomics
- Cancer Research and Treatments
- Peptidase Inhibition and Analysis
- Monoclonal and Polyclonal Antibodies Research
- Signaling Pathways in Disease
- Cellular Mechanics and Interactions
- RNA modifications and cancer
- Nanoplatforms for cancer theranostics
- SARS-CoV-2 and COVID-19 Research
- T-cell and B-cell Immunology
- Protein Degradation and Inhibitors
- Immune cells in cancer
- CRISPR and Genetic Engineering
- Epigenetics and DNA Methylation
- Cancer, Hypoxia, and Metabolism
Weizmann Institute of Science
2016-2022
// Shelly Kalaora 1 , Eilon Barnea 2,* Efrat Merhavi-Shoham 3,* Nouar Qutob Jamie K. Teer 4 Nilly Shimony 3 Jacob Schachter Steven A. Rosenberg 5 Michal J. Besser 3,6,** Arie Admon 2,** and Yardena Samuels Department of Molecular Cell Biology, Weizmann Institute Science, Rehovot, Israel 2 Technion, Haifa, The Ella Lemelbaum for Melanoma, Chaim Sheba Medical Center, Tel Hashomer, Biostatistics Bioinformatics, H. Lee Moffitt Cancer Center Research Institute, Tampa, FL, USA National NIH, MD, 6...
Abstract Predicting the outcome of immunotherapy treatment in melanoma patients is challenging. Alterations genes involved antigen presentation and interferon gamma (IFNγ) pathway play an important role immune response to tumors. We describe here that overexpression PSMB8 PSMB9 , two major components immunoproteasome, predictive better survival improved immune-checkpoint inhibitors patients. study mechanism underlying this connection by analyzing antigenic peptide repertoire cells...
The quest for tumor-associated antigens (TAA) and neoantigens is a major focus of cancer immunotherapy. Here, we combine neoantigen prediction pipeline human leukocyte antigen (HLA) peptidomics to identify TAAs in 16 tumors derived from seven patients with melanoma characterize their interactions tumor-infiltrating lymphocytes (TIL). Our investigation the antigenic T-cell landscapes encompassing TAA signatures, immune reactivity, corresponding identities provides first comprehensive analysis...
The human leukocyte antigen (HLA)-bound viral antigens serve as an immunological signature that can be selectively recognized by T cells. As viruses evolve acquiring mutations, it is essential to identify a range of presented antigens. Using HLA peptidomics, we are able severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-derived peptides highly prevalent class I (HLA-I) molecules using infected cells well overexpression SARS-CoV-2 genes. We find 26 HLA-I and 36 II (HLA-II) peptides....
Neoantigens are now recognized drivers of the antitumor immune response. Recurrent neoantigens, shared among groups patients, have thus become increasingly coveted therapeutic targets. Here, we report on data-driven identification a robustly presented, immunogenic neoantigen that is derived from combination HLA-A*01:01 and RAS.Q61K. Analysis large patient cohorts indicated this applies to 3% patients with melanoma. Using HLA peptidomics, were able demonstrate robust endogenous presentation...
NRAS mutations are the most common alterations among RAS isoforms in cutaneous melanoma, with patients harboring these aggressive tumors having a poor prognosis and low survival rate. The main line of treatment for is MAPK pathway-targeted therapies, such as MEK inhibitors, but, unfortunately, response to inhibitors variable due tumor resistance. Identifying genetic modifiers involved resistance toward MEK-targeted therapy may assist development new therapeutic strategies, enhancing patient...
// Michal Alon 1 , Rand Arafeh Joo Sang Lee 2, 3 Sanna Madan Shelly Kalaora Adi Nagler Tereza Abgarian Polina Greenberg Eytan Ruppin and Yardena Samuels Molecular Cell Biology Department, Weizmann Institute of Science, Rehovot, Israel 2 Center for Bioinformatics Computational Biology, The University Maryland, College Park, USA Cancer Data Science Lab, National Institute, Health, Bethesda, Correspondence to: Samuels, email: Yardena.samuels@weizmann.ac.il Keywords: melanoma; NF1; CAPN1;...
Introduction Studies in the past few years have suggested a key role for neo-antigens cancer immunotherapy. Since are specifically expressed on tumour, targeting them is not likely to induce tolerance or normal tissue toxicity, making candidates Despite encouraging results clinical trials using neo-antigens, peptide RNA vaccines and adoptive cell transfer (ACT), only handful of their corresponding T-cells been identified patients. Material methods In this study we combination novel...
<p>Supplementary methods</p>
<p>2P microscopy movies of in vivo experiments</p>
<p>In vitro killing assay movies. Co-culture of 12T melanoma cells with autologous 12TILs</p>
<p>In vitro killing assay movies. Co-culture of 12T melanoma cells with irrelevant 108TILs</p>
<p>Supplementary Figures 1-17</p>
<p>Supplementary methods</p>
<p>In vitro killing assay movies. Co-culture of 12T melanoma cells with autologous 12TILs</p>
<p>Supplementary Figures 1-17</p>