A5060041723- Blood transfusion and management
- Blood groups and transfusion
- Hemoglobinopathies and Related Disorders
- Neonatal Health and Biochemistry
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Erythrocyte Function and Pathophysiology
- Blood donation and transfusion practices
- Mechanical Circulatory Support Devices
- Blood disorders and treatments
- Platelet Disorders and Treatments
- Erythropoietin and Anemia Treatment
- Ubiquitin and proteasome pathways
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Ethics and Legal Issues in Pediatric Healthcare
- Vaccine Coverage and Hesitancy
- Nitric Oxide and Endothelin Effects
- Cardiac Arrest and Resuscitation
- Hyperglycemia and glycemic control in critically ill and hospitalized patients
- Atrial Fibrillation Management and Outcomes
- Environmental Monitoring and Data Management
- Cardiac Ischemia and Reperfusion
- Body Composition Measurement Techniques
- Species Distribution and Climate Change
- Prenatal Screening and Diagnostics
- Neutropenia and Cancer Infections
University of Washington
2015-2025
Seattle Children's Hospital
2018-2025
Bloodworks Northwest
2017-2021
Seattle University
2017-2018
Northwest University
2017-2018
Harborview Medical Center
2017
University of Washington Medical Center
2013
Washington University in St. Louis
2004
Importance Red blood cell transfusion is a common medical intervention with benefits and harms. Objective To provide recommendations for use of red in adults children. Evidence Review Standards trustworthy guidelines were followed, including using Grading Recommendations Assessment, Development Evaluation methods, managing conflicts interest, making values preferences explicit. from systematic reviews randomized controlled trials was reviewed. Findings For adults, 45 20 599 participants...
Oxidized lipoproteins may play an important role in the pathogenesis of atherosclerosis. Elevated levels 3-chlorotyrosine, a specific end product reaction between hypochlorous acid (HOCl) and tyrosine residues proteins, have been detected atherosclerotic tissue. Thus, HOCl generated by phagocyte enzyme myeloperoxidase represents one pathway for protein oxidation humans. One target be high density lipoprotein (HDL), which mobilizes cholesterol from artery wall cells. To determine whether...
Importance Red blood cell (RBC) transfusion is a common medical intervention to treat anemia in very preterm neonates; however, best practices, such as thresholds, remain uncertain. Objective To develop recommendations for clinicians on the use of RBC transfusions neonates. Evidence Review An international steering committee reviewed evidence from systematic review 6 randomized clinical trials (RCTs) that compared high vs low hemoglobin-based or hematocrit-based thresholds. The reached...
Matrix metalloproteinases (MMPs) function in homeostatic and repair processes, but unregulated catalysis by these extracellular proteinases leads to the pathological destruction of tissue proteins. An important mechanism for controlling enzyme activity might involve hypochlorous acid (HOCl), a potent oxidant produced myeloperoxidase system phagocytes. We have shown that inactivation MMP-7 (matrilysin) HOCl coincides with formation novel oxidation product, WG-4, through modification adjacent...
Abstract Alloimmunization is prevalent in patients with sickle cell disease (SCD) and can be a barrier to gene therapy (GT) due the necessary transfusion support for successful stem collection infusion. We estimate that standard‐of‐care GT an adult SCD will require average of 35–45 units red blood cells over 6‐month period. Institutions should actively plan these needs share information inform national consensus policies on management alloimmunization during GT.
Abstract Background Whether granulocytes for transfusion are beneficial remains uncertain, although some evidence suggests that efficacy may be dose‐related. Granulocytes mostly produced by apheresis procedure, but other means of production increasingly used. Methods Centers produce and/or use were recruited through the BEST Collaborative and completed a detailed survey granulocyte manufacture, specifications, clinical use, operational considerations, data collection initiatives. Results...
To balance the risk of bleeding versus circuit thrombosis during extracorporeal life support (ECLS), it is important to monitor anticoagulants and hemostasis. We evaluated prothrombin time (PT), partial thromboplastin (PTT), activated clotting (ACT), antifactor Xa heparin activity (aXa) correlation with changes in coagulation factor levels using vitro vivo samples. aXa correlated (r 2 = 0.97) antithrombin 0.98) but was unaffected by other parameters. PT factors 0.88) minimally affected or...
Abstract Background Hyperkalemia is a rare life‐threatening complication of red blood cell (RBC) transfusion. Stored RBCs leak intracellular potassium (K+) into the supernatant; irradiation potentiates K+ leak. As characteristics patients and implicated have not been studied systematically, multicenter study transfusion‐associated hyperkalemia (TAH) in pediatric population was conducted through AABB Pediatric Transfusion Medicine Subsection. Study Design The medical records <18 years old...
Senataxin is a large 303 kDa protein linked to neuron survival, as recessive mutations cause Ataxia with Oculomotor Apraxia type 2 (AOA2), and dominant amyotrophic lateral sclerosis 4 (ALS4). contains an amino-terminal protein-interaction domain carboxy-terminal DNA/RNA helicase domain. In this study, we focused upon the common ALS4 mutation, L389S, by performing yeast two-hybrid screens of human brain expression library control senataxin or L389S bait. Interacting clones identified from two...
Abstract Background Pediatric patients on extracorporeal membrane oxygenation (ECMO) often receive repeated red blood cell (RBC) transfusions. This study aims to quantify and characterize causes of RBC loss ECMO. Methods retrospective, single‐center, observational includes 91 ECMO (age 1 day–20 years). An index (RLI), equal ml RBCs lost per liter patient + circuit volume hour, was calculated from the changes in hematocrit transfused RBCs. To measure contribution injury/activation,...
Wrong blood in tube (WBIT) errors can lead to ABO mistransfusions. It is unknown if WBIT are more likely specific healthcare locations or collection practices influence the commission of errors.Data on pretransfusion samples from calendar year 2019 were collected retrospectively by 39 transfusion services nine countries. We compared proportion made emergency departments (EDs), inpatient wards, and outpatient clinics.In total, 143 detected among 1,394,862 for an unadjusted aggregate...
Body fluid specimens other than serum, plasma or urine are generally not validated by manufacturers, but analysis of these non-standard fluids can be important for clinical diagnosis and management. Laboratories, therefore, rely on the published literature to better understand validation implementation such tests. This study utilized a data-driven approach determine reportable range 11 analytes, evaluated total bilirubin assay, assessed interferences from hemolysis, icterus, lipemia in...
BACKGROUND Treatment of necrotizing soft tissue infections (NSTIs) includes prompt surgical debridement and antibiotics, but despite standard care, the morbidity mortality remain high. Since therapeutic plasma exchange (TPE) has been considered for treatment severe sepsis, this study evaluates efficacy TPE patients with NSTI. STUDY DESIGN AND METHODS This is a retrospective diagnosis NSTI who received without over an 11‐year period. The primary outcome was in‐hospital mortality. RESULTS...
The authors declare no conflicts of interest relevant to this work.
Using a multidisciplinary approach and simulation, massive transfusion process (MTP) was developed to care for patients in need of emergency transfusion. It then assessed effectiveness.After series sentinel bleeding events, reliable hospital staff deliver appropriate blood products obtain relevant laboratory tests guide therapy with needed.To determine the feasibility new MTP, teams participated simulation events. Each event helped refine MTP. A special testing panel devised. To judge...
Indication for
Abstract Background Transfusion‐associated hyperkalemia (TAH) is a potentially life‐threatening complication of red blood cell (RBC) transfusion. Previously, we reported features RBC transfusions from 35 pediatric patients (TAH group) who had with transfusion in one‐year period at four facilities. In this study, used multivariate analyses and artificial intelligence to compare the TAH group newly collected control (non‐TAH identify factors associated occurrence. Study Design A review was...
A 41-year-old man presented to the emergency department with auxiliary swelling and pain, fever, diarrhea, chills.He was admitted for rapid onset of respiratory failure, anuria, lactic acidosis, shock, coagulopathy.The laboratory received 2 samples ordered 9 h apart hepatitis B virus (HBV) serology analysis.Discrepant HBV anti-surface anti-core antibody results (Fig. 1, arrows) prompted additional testing.Surface antigen (not shown) not detected in any samples.QUESTIONS 1.For acute...