A5045122607- Bacterial biofilms and quorum sensing
- Enzyme Structure and Function
- Antimicrobial Resistance in Staphylococcus
- Plant nutrient uptake and metabolism
- Legume Nitrogen Fixing Symbiosis
- Bacterial Genetics and Biotechnology
- Biochemical and Structural Characterization
- Heat shock proteins research
- Antibiotic Resistance in Bacteria
- Ubiquitin and proteasome pathways
- Protein Structure and Dynamics
- Heme Oxygenase-1 and Carbon Monoxide
- SARS-CoV-2 and COVID-19 Research
- Antimicrobial Peptides and Activities
- 14-3-3 protein interactions
- COVID-19 Clinical Research Studies
- Iron Metabolism and Disorders
- Glycosylation and Glycoproteins Research
- Hemoglobin structure and function
- Viral Infections and Outbreaks Research
- DNA and Nucleic Acid Chemistry
- Synthesis and biological activity
- Biofuel production and bioconversion
- interferon and immune responses
- Microbial Metabolism and Applications
Sungkyunkwan University
2010-2025
Sookmyung Women's University
2021
Samsung Medical Center
2018
Samsung (South Korea)
2014-2016
Escherichia coli SdiA is a quorum-sensing (QS) receptor that responds to autoinducers produced by other bacterial species control cell division and virulence. Crystal structures reveal E. SdiA, which composed of an N-terminal ligand-binding domain C-terminal DNA-binding (DBD), forms symmetrical dimer. Although each shows structural similarity QS receptors, differs from them in the relative orientation two domains, suggesting its functions are independent. Consistently, DNA gel-shift assays...
Due to the shortage of effective antibiotics against drug-resistant Staphylococcus aureus , new targets are urgently required develop next-generation antibiotics. We investigated mannitol-1-phosphate dehydrogenase S. USA300 ( Sa M1PDH), a key enzyme regulating intracellular mannitol levels, and explored possibility using M1PDH as target for developing antibiotic. Since is necessary maintaining cellular redox osmotic potential, homeostatic imbalance caused by treatment with inhibitor or...
Abstract Background The rapid emergence of multiple drug-resistant (MDR) bacterial pathogens and the lack a novel antibiotic pipeline pose serious threat to global healthcare. limited number established targets further restricts identification antibiotics treat life-threatening MDR infections caused by Staphylococcus aureus strains. Therefore, for developing are urgently required. In this study, we hypothesized that G-quadruplex (G4)-binding ligands can be used as their binding possibly...
Cryogenic electron microscopy (cryo-EM) has revolutionized structural biology by enabling the study of macromolecular structures in near-native conditions, suspended vitreous ice. This technique allows for high-resolution visualization proteins and other biomolecules without need crystallization, offering significant insights into their function mechanism. Recent advancements single-particle analysis, coupled with improved computational data processing, have made cryo-EM an indispensable...
Dysregulation of deubiquitinating enzymes (DUBs), which regulate the stability key proteins, has been implicated in many human diseases, including cancers. Thus, DUBs can be considered as potential therapeutic targets for diseases. Among them, USP4 proposed a promising target colon cancer drugs since controls β-catenin, factor Wnt signaling involved tumorigenesis colorectal cancer. However, developing DUB inhibitors hindered because harbor similar active site structures and show broad...
UDP-glucose pyrophosphorylases (UGPase; EC 2.7.7.9) catalyze the conversion of UTP and glucose-1-phosphate to pyrophosphate vice versa. Prokaryotic UGPases are distinct from their eukaryotic counterparts considered appropriate targets for development novel antibacterial agents since product, UDP-glucose, is indispensable biosynthesis virulence factors such as lipopolysaccharides capsular polysaccharides. In this study, crystal structures UGPase Helicobacter pylori (HpUGPase) were determined...
The emergence of bactericidal antibiotic-resistant strains has increased the demand for alternative therapeutic agents, such as antivirulence agents targeting virulence regulators pathogens. Staphylococcus aureus exoprotein expression (sae) locus, master regulator gene in multiple drug-resistant S. aureus, is a promising target. In this study, we screened small-molecule library using SaeRS green fluorescent protein (GFP)-reporter that responded to transcription controlled by sae locus. We...
Bacterial pathogens have evolved diverse types of efficient machinery to acquire haem, the most abundant source iron in human body, and degrade it for utilization iron. Gram-positive bacteria commonly encode IsdG-family proteins as haem-degrading monooxygenases. Listeria monocytogenes is predicted possess an IsdG-type protein (Lmo2213), but residues involved haem monooxygenase activity are not well conserved there extra N-terminal domain Lmo2213. Therefore, its function mechanism action...
As an alternative strategy to fight antibiotic resistance, two-component systems (TCSs) have emerged as novel targets. Among TCSs, master virulence regulators that control the expression of multiple factors are considered excellent antivirulence In Staphylococcus aureus, factor is tightly regulated by a few regulators, including SaeRS TCS. this study, we used GFP-reporter system screen natural compound inhibitors SaeRS, and identified xanthoangelol B 1, prenylated chalcone from Angelica...
Carbohydrate-active enzymes (CAZymes) regulate the synthesis, degradation, and modification of poly—and oligosaccharides in all three kingdoms life. A novel carbohydrate acetylesterase from Sinorhizobium meliloti, designated SmAcE1, was identified, characterized, crystallized. This SmAcE1 is classified into esterase family 3 (CE3) based on sequence alignments with other currently known (CE) enzymes. The crystallized as a hexamer space group P212121 unit cell parameters: = 99.12 Å, b 148.88 c...
Methanocaldococcus jannaschii, a hyperthermophilic and barophilic methanarchaeon, contains single gene (MJ0285) encoding 16.5-kDa polypeptide chain of small heat-shock protein (sHSP). This sHSP—now called MjsHSP16.5—is upregulated in response to high growth temperature or pressure (1, 2) functions as broad substrate ATP-independent holding chaperone that transiently binds prevents the misfolded proteins from aggregation (3-7). Despite being extensively studied for decades, molecular...
Green chemistry paradigm has been raised to reduce environmental damages during process of products.American Chemical Society suggested 12 principals such as atom economy, safter chemicals, energy efficiency, degradable products and less hazardous chemical syntheses.One alternatives for green is using biocatalysts, whose endogenous characters are attractive in the following aspects: decrease synthesis procedures, side products, mild reaction condition.Microbial enzymes one promising sources...