A5070783510- Autophagy in Disease and Therapy
- Pancreatic function and diabetes
- Cannabis and Cannabinoid Research
- Diet, Metabolism, and Disease
- Mitochondrial Function and Pathology
- CRISPR and Genetic Engineering
- Parkinson's Disease Mechanisms and Treatments
- interferon and immune responses
- Virus-based gene therapy research
- Endoplasmic Reticulum Stress and Disease
- Stroke Rehabilitation and Recovery
- Electrocatalysts for Energy Conversion
- Health Systems, Economic Evaluations, Quality of Life
- Neuroscience and Neuropharmacology Research
- Retinal Development and Disorders
- bioluminescence and chemiluminescence research
- RNA modifications and cancer
- Microtubule and mitosis dynamics
- Advanced Fluorescence Microscopy Techniques
- Pluripotent Stem Cells Research
- DNA Repair Mechanisms
- Ubiquitin and proteasome pathways
- RNA Interference and Gene Delivery
- Acute Ischemic Stroke Management
- Mechanisms of cancer metastasis
Doctors Hospital
2025
University of Colorado Boulder
2016-2024
Duke University
2016
Sorafenib (Nexavar) is a broad-spectrum multikinase inhibitor that proves effective in treating advanced renal-cell carcinoma and liver cancer. Despite its well-characterized mechanism of action on several established cancer-related protein kinases, sorafenib causes variable responses among human tumors, although the cause for this variation unknown. In an unbiased screening oncology drug library, we found activates recruitment ubiquitin E3 ligase Parkin to damaged mitochondria. We show...
•Cannabidiol (CBD) treatment elicits a broad spectrum of intracellular phenotypes.•Cytosolic and ER calcium homeostasis is disturbed by CBD.•CBD incorporates into membranes alters cholesterol accessibility.•Cholesterol biosynthesis transport signaling increased CBD.•Lateral diffusion within decreased CBD. The nonpsychoactive cannabinoid, cannabidiol (CBD), Food Dug Administration approved for two drug-resistant epileptic disorders seeing use among the general public, yet mechanisms that...
Background: A successful clinical trial requires retention as well recruitment of subjects. Exploring reasons for dropout can provide insights application to the design and methods future long-term stroke trials. The aim this study is understand in a trial. Methods: C3FIT (Coordinated, Collaborative, Comprehensive Family-Based, Integrated, Technology-enabled Care) (NCT04000971) multicenter, randomized, single-blinded, Phase III, cluster randomized assessing if an Integrated Stroke Practice...
Targeting mitotic kinase monopolar spindle 1 (Mps1) for tumor therapy has been investigated many years. Although it was suggested that Mps1 regulates cell viability through its role in assembly checkpoint (SAC), the underlying mechanism remains less defined. In an endeavor to reveal of high levels development colon cancer, we unexpectedly found amount required survival far exceeds maintaining SAC aneuploid lines. This suggests other functions besides are also employed maintain viability....
The PTEN-induced kinase 1 (PINK1)-Parkin pathway plays a vital role in maintaining healthy pool of mitochondria higher eukaryotic cells. While the downstream components this are well understood, upstream triggers remain less explored. In study, we conducted an extensive analysis inhibitors targeting various mitochondrial electron transport chain (ETC) complexes to investigate their potential as activators PINK1–Parkin pathway. We identified cloflucarban, antibacterial compound, novel...
Abstract The non-psychoactive cannabinoid, cannabidiol (CBD), is FDA-approved for treatment of two drug-resistant epileptic disorders, and seeing increased use among the general public, yet mechanisms that underlie its therapeutic effects side-effect profiles remain unclear. Here, we report a systems-level analysis CBD action in human cell lines using temporal multi-omic profiling. FRET-based biosensor screening revealed resulted sharp rise cytosolic calcium, activation AMPK ERK kinases...
Neurodegenerative disorders like Parkinson's Disease arise in part due to accumulation of unhealthy mitochondria. PINK1 and Parkin, found be deficient some Familial patients, are known orchestrate mitochondrial quality control responses mitophagy. Research into Parkin function has been focused primarily on downstream signaling events, but the exact type damage signals originating from defected mitochondria trigger stabilization subsequent recruitment outer membrane damaged remains elusive....
Abstract Inflammatory breast cancer (IBC) is the most lethal, distinct form of cancer, however, basis for its aggressiveness and rapid acquisition drug resistance not fully understood. Using immunohistochemical analysis, we identified a strong correlation between high grade, stage, triple-negative status elevated expression X-linked inhibitor apoptosis protein (XIAP) in IBC. Molecular profiling multiple IBC cell lines revealed that modulating XIAP can significantly alter pattern 79-gene,...