A5046664817- Pancreatic function and diabetes
- Pluripotent Stem Cells Research
- Diabetes Management and Research
- Blood Pressure and Hypertension Studies
- Diabetes and associated disorders
- Esophageal and GI Pathology
- Genetic Syndromes and Imprinting
- Vasculitis and related conditions
- Tracheal and airway disorders
- Abdominal vascular conditions and treatments
- Renal and Vascular Pathologies
- 3D Printing in Biomedical Research
- Kruppel-like factors research
- Gastroesophageal reflux and treatments
- Congenital heart defects research
- CRISPR and Genetic Engineering
- Nutritional Studies and Diet
- Lipoproteins and Cardiovascular Health
Administração Regional de Saúde de Lisboa e Vale do Tejo
2025
Hospital de São João
2010-2018
University of Aberdeen
2012-2016
Because of the lack tissue available for islet transplantation, new sources β-cells have been sought treatment type 1 diabetes. The aim this study was to determine whether human exocrine-enriched fraction from isolation procedure could be reprogrammed provide additional transplantation. cells rapidly dedifferentiated in culture and grew as a mesenchymal monolayer. Genetic lineage tracing confirmed that these arose, part, through process epithelial-to-mesenchymal transitioning (EMT). A...
Transcription factor mediated lineage reprogramming of human pancreatic exocrine tissue could conceivably provide an unlimited supply islets for transplantation in the treatment diabetes. Exocrine can be efficiently reprogrammed to islet-like cells using a cocktail transcription factors: Pdx1, Ngn3, MafA and Pax4 combination with growth factors. We show here that overexpression exogenous suppression endogenous ARX considerably enhances production functional insulin-secreting β-like...
In renal vasculitis, luminal narrowing can reduce blood flow and activate the renin-angiotensin-aldosterone system, causing renovascular hypertension. We present case of a 47-year-old man with previous intestinal tuberculosis episodes lumbar pain, tender erythematous nodules arthralgias. He had grade 3 hypertension, unresponsive to treatment, left ventricular concentric hypertrophy chronic kidney disease. was admitted ICU due hypertensive emergency, acute failure large peri-renal hematoma....
A replenishable source of insulin-producing cells has the potential to cure type 1 diabetes. Attempts culture and expand pancreatic β-cells in vitro have resulted their transition from epithelial mesenchymal stromal (MSCs) with high proliferative capacity but devoid any hormone production. The aim this study was determine whether transcription factor Krüppel-like 4 (KLF4), could induce a mesenchymal-to-epithelial (MET) cultured cells. Islet-enriched cells, allowed dedifferentiate adherent...
Protein transduction domains (PTDs), such as the HIV1-TAT peptide, have been previously used to promote uptake of proteins into a range cell types, including stem cells. Here we generated pancreatic transcription factors containing PTD sequences and administered these endoderm enriched mouse embryonic (ES) cells under conditions that were designed mimic pattern expression in developing pancreas. The ES first cultured embryoid bodies treated with Activin A Bone morphogenetic protein 4 (BMP4)...
Objective: To determine the factors associated with resistant hypertension (HTN). Methods: We selected a sample of 96 consecutive patients attending HTN consultation in public hospital. collected data on demographic variables, cardiometabolic diseases, antihypertensive, statins and antithrombotic drugs. Age was grouped less than 65 years or older. Obesity defined as body mass index equal greater to 30. Resistant considered blood pressure above 140/90mmHg last visit spite use 3 more...
Paulo, C.; Pereira, S.; Pinho, E.; Mascarenhas, J.; Silva, L.; Ferreira, Santos, R.; M.; Flores, A.; Vilas-Boas, Brás, V.; Lima, M. J. Author Information
Objective: The evaluation and follow up of hypertensive patients requires the consideration all cardiovascular risk factors such as lipid profile, particularly LDL cholesterol. Hypertensive usually gather other that make them high very requiring aggressive control. We aimed to stratify profile from evaluated during a year their Design method: Retrospective study based on clinical records in Hypertension clinic tertiary care hospital year. Demographic, biometric data were gathered. used...