A5023141960- Redox biology and oxidative stress
- Nitric Oxide and Endothelin Effects
- Enzyme Structure and Function
- Photosynthetic Processes and Mechanisms
- Aldose Reductase and Taurine
- Heat shock proteins research
- Plant Stress Responses and Tolerance
- Advanced Glycation End Products research
- Phytoestrogen effects and research
- Genomics, phytochemicals, and oxidative stress
- Plant nutrient uptake and metabolism
- thermodynamics and calorimetric analyses
- Water Quality Monitoring and Analysis
- Glutathione Transferases and Polymorphisms
- Reproductive Biology and Fertility
- Birth, Development, and Health
- Plant Molecular Biology Research
- Soybean genetics and cultivation
- Circadian rhythm and melatonin
- Electron Spin Resonance Studies
- Heme Oxygenase-1 and Carbon Monoxide
- Sulfur Compounds in Biology
- Calcium Carbonate Crystallization and Inhibition
- Biotin and Related Studies
- Biochemical effects in animals
University of Massachusetts Amherst
2018-2024
Amherst College
2022
Bielefeld University
2011-2021
Highly reactive carbonyl compounds formed during glycolysis, such as methylglyoxal (MG), can lead to the formation of 'advanced glycation end products' (AGE) and stress. Toxic AGEs are suspected accumulate play a role in reducing quality developmental potential mammalian oocytes aged females PCOS diabetic patients. Whether how MG AGE affect young at cellular level is unknown. The study consists three parts. In Part A expression MG-detoxifying enzymes glyoxalases 1 2 was analysed by RT–PCR...
Nitric oxide (NO) is a short-lived radical gas that acts as signaling molecule in all higher organisms, and involved multiple plant processes, including germination, root growth, fertility. Regulation of NO-levels predominantly achieved by reaction oxidation products NO with glutathione to form S-nitrosoglutathione (GSNO), the principal bioactive NO. The enzyme reductase (GSNOR) major route NADH-dependent GSNO catabolism critical homeostasis. Here, we performed proteomic analysis examining...
Peroxiredoxins (PRX) are thiol peroxidases that highly conserved throughout all biological kingdoms. Increasing evidence suggests their high reactivity toward peroxides has a function not only in antioxidant defense but particular redox regulation of the cell. Peroxiredoxin IIE (PRX-IIE) is one three PRX types found plastids and previously been linked to pathogen protection from protein nitration. However, its posttranslational chloroplast network remained be explored. Using recombinant...
Plant fertility is fundamental to plant survival and requires the coordinated interaction of developmental pathways signaling molecules. Nitric oxide (NO) a small, gaseous molecule that plays crucial roles in as well other processes stress responses. NO influences biological through S-nitrosation, posttranslational modification protein cysteines S-nitrosocysteine (R-SNO). homeostasis controlled by S-nitrosoglutathione reductase (GSNOR), which reduces (GSNO), major form cells. GSNOR mutants...
SUMMARY Alcohol dehydrogenases (ADHs) are a group of zinc‐binding enzymes belonging to the medium‐length dehydrogenase/reductase (MDR) protein superfamily. In plants, these fulfill important functions involving reduction toxic aldehydes corresponding alcohols (as well as catalyzing reverse reaction, i.e., alcohol oxidation; ADH1) and nitrosoglutathione (GSNO; ADH2/GSNOR). We investigated compared structural biochemical properties ADH1 GSNOR from Arabidopsis thaliana . expressed purified...
Abstract Plant fertility is fundamental to plant survival and requires the coordinated interaction of developmental pathways signaling molecules. Nitric oxide (NO) a small gaseous molecule that plays crucial roles in as well other processes stress responses. NO influences biological through S-nitrosation, posttranslational modification protein cysteines S-nitrosocysteine (R-SNO). homeostasis controlled by S-nitrosoglutathione reductase (GSNOR), which reduces (GSNO), major form cells. GSNOR...
Nitric oxide (NO) is a short-lived gas that acts as signaling molecule in all higher organisms, including plants. Despite the involvement of NO multiple plant processes, germination, root growth and fertility, basic understanding mechanisms by which exerts its effects lacking. derivatives impact these physiological processes through reversible S-nitrosation critical protein cysteines. In cells, regulation NO-levels predominantly achieved reaction reactive nitrogen species (RNS) with...
In plants, nitric oxide (NO) is a crucial signaling molecule involved in many physiological processes and stress pathways. NO can influence plant germination, flowering, root development1 . usually found an adduct with glutathione, forming S-nitrosoglutathione (GSNO), which affect protein S-nitrosation, important post-translational modification that alter activity stability. The enzyme reductase (GSNOR) catalyzes the irreversible NADH-dependent reduction of GSNO. GSNOR's ability to regulate...
Abstract Peroxiredoxins (PRX) are thiol peroxidases which highly conserved throughout all biological kingdoms. Increasing evidence suggests that their high reactivity toward peroxides has a function not only in antioxidant defense but particular redox regulation of the cell. Peroxiredoxin IIE is one three PRX types found plastids and previously been linked to pathogen protection from protein nitration. However, its posttranslational chloroplast network remained be explored. Using recombinant...