A5084644009- Mass Spectrometry Techniques and Applications
- Protein Structure and Dynamics
- Analytical Chemistry and Chromatography
- Enzyme Structure and Function
- Bacterial Genetics and Biotechnology
- Metabolomics and Mass Spectrometry Studies
- Genetics, Bioinformatics, and Biomedical Research
- Alzheimer's disease research and treatments
- Ubiquitin and proteasome pathways
- Various Chemistry Research Topics
- Hemoglobin structure and function
- Receptor Mechanisms and Signaling
- Protein Interaction Studies and Fluorescence Analysis
- Cancer, Hypoxia, and Metabolism
- Endoplasmic Reticulum Stress and Disease
- Glycosylation and Glycoproteins Research
- Advanced Proteomics Techniques and Applications
- Autophagy in Disease and Therapy
- RNA and protein synthesis mechanisms
- Chemical Reactions and Isotopes
- Heat shock proteins research
- Bioinformatics and Genomic Networks
- Neuropeptides and Animal Physiology
- Lipid Membrane Structure and Behavior
- Antibiotic Resistance in Bacteria
University of Massachusetts Amherst
2012-2023
Eunice Kennedy Shriver National Institute of Child Health and Human Development
2023
Amherst College
2022
Boston University
2001
Bruker (United States)
2000
University of Oxford
1992-1994
CD1c-mediated T cells are activated by a mycobacterial phospholipid antigen whose carbohydrate structure precisely corresponds to mammalian mannosyl β-1-phosphodolichol (MPD), but contains an unusual lipid moiety. Here, we show that this cell is member of family branched, alkane lipids vary in length (C30-34) and produced medically important mycobacteria such as M. tuberculosis bovis Bacille-Calmette-Guerin. The moiety distinguished these antigens from MPDs was necessary for activation...
Protein amide hydrogen exchange (HDX) is a convoluted process, whose kinetics determined by both dynamics of the protein and intrinsic rate labile atoms fully exposed to solvent. Both processes are influenced variety extrinsic factors. A mathematical formalism initially developed rationalize individual now often used interpret global (e.g., as measured in HDX MS experiments). One particularly important advantage direct visualization various states observing distinct ion populations with...
Vulcanized rubbers have three-dimensional chemical networks, and as a result they do not melt or dissolve. The presence of these networks creates tremendous problem at the end product's life (i.e., recycling). Recently, R. J. Farris et al. rediscovered technique coined "high-pressure high-temperature sintering" (HPHTS) that fuses/sinters 100% vulcanized rubber powder into solid mass recovering approximately 35−40% original mechanical properties. A method enhancing properties sintered natural...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTKinetic Consequences of the Removal a Disulfide Bridge on Folding Hen LysozymeStephen J. Eyles, Sheena E. Radford, Carol V. Robinson, and Christopher M. DobsonCite this: Biochemistry 1994, 33, 44, 13038–13048Publication Date (Print):November 8, 1994Publication History Published online1 May 2002Published inissue 8 November 1994https://pubs.acs.org/doi/10.1021/bi00248a013https://doi.org/10.1021/bi00248a013research-articleACS PublicationsRequest reuse...
Degradation by the 26 S proteasome is an intricately regulated process fine tuned precise nature of ubiquitin modifications attached to a protein substrate. By debranching chains composed K48 linkages, proteasome-associated C-terminal hydrolase UCHL5/UCH37 serves as positive regulator degradation. How UCH37 achieves specificity for unclear. Here, we use combination hydrogen-deuterium mass spectrometry, chemical crosslinking, small-angle X-ray scattering, nuclear magnetic resonance (NMR),...
We have probed the conformational stability of cellular retinoic acid-binding protein I, a predominantly β-sheet protein, using hydrogen/deuterium (H/D) exchange in solution. Transiently populated intermediate states were detected H/D measurement under mildly denaturing conditions (pH 2.5 and room temperature). By inducing collisionally activated dissociation nozzle-skimmer region electrospray source an FT ICR mass spectrometer (MS), residue-specific information was obtained as to degree...
Aggregation of amyloid-β (Aβ) peptides is a crucial step in the progression Alzheimer's disease (AD). Identifying aggregation inhibitors against AD has been great challenge. We report an atomistic simulation study inhibition mechanism two small molecules, homotaurine and scyllo-inositol, which are drug candidates currently under investigation. show that both molecules promote conformational change Aβ42 monomer toward more collapsed phase through nonspecific binding mechanism. This finding...
Analysis of protein ion charge-state distributions in electrospray ionization (ESI) mass spectra has become an indispensable tool the studies dynamics. However, applications this technique have been thus far limited to detection large-scale conformational transitions, which typically change extent multiple charging a very significant way. more subtle changes often elude detection, since resulting are smaller than shifts caused by other external factors. Proton-transfer reactions involving...
HIF-asparaginyl hydroxylase (FIH-1) normally couples O2-activation to hydroxylation of Asn803 on the α-subunit hypoxia-inducible factor (HIFα), a key step in pO2 sensing; absence HIFα, becomes uncoupled, leading self-hydroxylation at Trp296 and purple Fe(III)–O–Trp chromophore—this alternative reactivity may affect human hypoxia sensing.
Cation-π interactions, where protein aromatic residues supply π systems while a positive-charged portion of phospholipid head groups are the cations, have been suggested as important binding modes for peripheral membrane proteins. However, amino acids can also insert into membranes and hydrophobically interact with lipid tails. Heretofore there has no facile way to differentiate these two types interactions. We show that specific incorporation fluorinated proteins experimentally distinguish...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTCooperative Elements in Protein Folding Monitored by Electrospray Ionization Mass SpectrometryShaun D. Hooke, Stephen J. Eyles, Andrew Miranker, Sheena E. Radford, Carol V. Robinson, and Christopher M. DobsonCite this: Am. Chem. Soc. 1995, 117, 28, 7548–7549Publication Date (Print):July 1, 1995Publication History Published online1 May 2002Published inissue 1 July 1995https://doi.org/10.1021/ja00133a030Request reuse permissionsArticle...
Diethylpyrocarbonate (DEPC)-based covalent labeling together with mass spectrometry is a promising tool for the higher-order structural analysis of antibody therapeutics. Reliable information about structure can be obtained, though, only when protein's integrity preserved during labeling. In this work, we have evaluated applicability DEPC reaction kinetics ensuring monoclonal antibodies (mAbs) By monitoring modification extent selected proteolytic fragments as function concentration, find...