A5103090543- Plant Reproductive Biology
- Plant Molecular Biology Research
- SARS-CoV-2 and COVID-19 Research
- Photosynthetic Processes and Mechanisms
- COVID-19 Clinical Research Studies
- Vaccine Coverage and Hesitancy
- Antibiotic Resistance in Bacteria
- Bacterial biofilms and quorum sensing
- Plant tissue culture and regeneration
- Vibrio bacteria research studies
- Plant biochemistry and biosynthesis
- Heparin-Induced Thrombocytopenia and Thrombosis
- Exercise and Physiological Responses
- Cancer-related gene regulation
- Thermoregulation and physiological responses
- Muscle metabolism and nutrition
- Lung Cancer Treatments and Mutations
- Polysaccharides and Plant Cell Walls
- vaccines and immunoinformatics approaches
- Prostate Cancer Treatment and Research
- Intramuscular injections and effects
- Infections and bacterial resistance
- Plant and animal studies
- Wnt/β-catenin signaling in development and cancer
- Cancer-related Molecular Pathways
Taipei Medical University
2017-2025
Taipei Medical University Hospital
2016-2025
University of Dental Medicine
2024
University of Massachusetts Amherst
2015-2023
National Chung Hsing University
2007-2019
Institute of Biomedical Sciences, Academia Sinica
2018
University of Massachusetts System
2018
National Taiwan University
2011-2016
University of Massachusetts Boston
2015
Highlights•FER is necessary to maintain cell-wall integrity during the salt-stress response•Pectin cross-linking protects cell walls against damage induced by salinity•The extracellular domain of FER interacts directly with pectin•FER induces cell-specific [Ca2+] transients that integritySummaryCells despite changes in their mechanical properties elicited growth and environmental stress. How cells sense physical state compensate for poorly understood, particularly plants. Here we report...
In flowering plants, fertilization requires complex cell-to-cell communication events between the pollen tube and female reproductive tissues, which are controlled by extracellular signaling molecules interacting with receptors at surface. We found that two such in Arabidopsis, BUPS1 BUPS2, their peptide ligands, RALF4 RALF19, tube-expressed required to maintain integrity. BUPS2 interact ANXUR1 ANXUR2 via ectodomains, both sets of bind RALF19. These receptor-ligand interactions competition...
The Arabidopsis receptor kinase FERONIA (FER) is a multifunctional regulator for plant growth and reproduction. Here we report that the female gametophyte-expressed glycosylphosphatidylinositol-anchored protein (GPI-AP) LORELEI seedling-expressed LRE-like GPI-AP1 (LLG1) bind to extracellular juxtamembrane region of FER show this interaction pivotal function. LLG1 interacts with in endoplasmic reticulum on cell surface, loss function induces cytoplasmic retention FER, consistent transport...
BackgroundThe Delta and Omicron variants of SARS-CoV-2 are currently responsible for breakthrough infections due to waning immunity. We report phase I/II trial results UB-612, a multitope subunit vaccine containing S1-RBD-sFc protein rationally designed promiscuous peptides representing sarbecovirus conserved helper T cell cytotoxic lymphocyte epitopes on the nucleocapsid (N), membrane (M), spike (S2) proteins.MethodWe conducted I primary 2-dose (28 days apart) 10, 30, or 100 μg UB-612 in 60...
ABSTRACT Polymyxins, which are increasingly being used to treat infections caused by multidrug-resistant bacteria, perform poorly against Serratia marcescens . To investigate the underlying mechanisms, Tn 5 mutagenesis was performed and two mutants exhibiting increased polymyxin B (PB) susceptibility were isolated. The found have inserted into arnB arnC genes. In other belong seven-gene arn operon, is involved in lipopolysaccharide (LPS) modification. LPSs of had greater PB-binding abilities...
Background: Castration-resistant prostate cancer (PCa; CRPC) has a poor response to androgen deprivation therapy and is considered an incurable disease. MicroRNA (miR)-lethal 7c (let-7c) was implied be tumor suppressor in PCa, treatment with exogenous let-7c targets both cells their associated mesenchymal stem (MSCs) prevent CRPC progression metastasis. Exosomes are nanometer-sized membrane-bound vesicles which have absolute predominance biocompatibility for drug delivery gene by mediating...
ABSTRACT Proteus mirabilis is known to be highly resistant the action of polymyxin B (PB). However, mechanism underlying PB resistance not clear. In this study, we used Tn 5 transposon mutagenesis identify genes that may affect in P. . Two genes, ugd and galU , which encode UDP-glucose dehydrogenase (Ugd) pyrophosphorylase (GalU), respectively, were identified. Knockout mutants found extremely sensitive PB, presumably because alterations lipopolysaccharide (LPS) structure cell surface...
In gut epithelium, IL-22 transmits signals through STAT3 phosphorylation (pSTAT3) which provides intestinal immunity. Many components in the IL-22-pSTAT3 pathway have been identified as risk factors for inflammatory bowel disease (IBD) and some of them are considered promising therapeutic targets. However, new perspectives still needed to understand signaling effective clinical interventions IBD patients. Here, we revealed activating transcription factor 3 (ATF3), recently be upregulated...
Abstract Traditional thrombolytic therapeutics for vascular blockage are affected by their limited penetration into thrombi, associated off‐target side effects, and low bioavailability, leading to insufficient efficacy. It is hypothesized that these limitations can be overcome the precisely controlled targeted delivery of therapeutics. A theranostic platform developed biocompatible, fluorescent, magnetic, well‐characterized, with multiple targeting modes. This multimodal system remotely...
ABSTRACT Proteus mirabilis is naturally resistant to polymyxin B (PB). To investigate the underlying mechanisms, Tn 5 mutagenesis was performed, and a mutant exhibiting increased PB susceptibility isolated. The found have inserted into P pmrI ( ) gene, gene which may encode UDP-glucuronic acid decarboxylase. In other bacteria, belongs seven-gene pmrF operon, involved in lipopolysaccharide (LPS) modification. While knockout had wild-type LPS profile produced amounts of similar those by wild...
Trigonelline (TGN), an alkaloid identified in medicinal plants such as coffee (Coffea spp.) and fenugreek (Trigonella foenum-graecum), has demonstrated significant anticancer properties across various malignancies, yet its efficacy bladder cancer (BLCA) remains underappreciated. This study investigates TGN's role modulating stem cells (CSCs) the tumor microenvironment (TME), two key contributors to BLCA progression chemoresistance. Through comprehensive bioinformatics analyses of patient...
Fine airborne particulate matter (PM2.5) has adverse effects on human health. Assessing the long-term of PM2.5 exposure health and ecology is often limited by a lack reliable measurements. In Taipei, levels were not systematically measured until August, 2005. Due to popularity geographic information systems (GIS), landuse regression method been widely used in spatial estimation PM concentrations. This accounts for potential contributing factors local environment, such as traffic volume....
Hfq is a bacterial RNA chaperone involved in the riboregulation of diverse genes via small noncoding RNAs. Here, we show that critical for uropathogenic Proteus mirabilis to effectively colonize bladder and kidneys murine urinary tract infection (UTI) model establish burned wound rats. In this regard, found hfq mutant induced higher IL-8 MIF levels uroepithelial cells displayed reduced intra-macrophage survival. The loss affected abilities handle H2O2 osmotic pressures grow at 50°C. Relative...
Stenotrophomonas maltophilia, a gram-negative bacterium, has increasingly emerged as an important nosocomial pathogen. It is well-known for resistance to variety of antimicrobial agents including cationic polypeptides (CAPs). Resistance polymyxin B, kind CAPs, known be controlled by the two-component system PhoPQ. To unravel role PhoPQ in B S. phoP mutant was constructed. We found MICs chloramphenicol, ampicillin, gentamicin, kanamycin, streptomycin and spectinomycin decreased 2–64 fold...
A signaling complex comprising members of the LORELEI (LRE)-LIKE GPI-anchored protein (LLG) and Catharanthus roseus RECEPTOR-LIKE KINASE 1-LIKE (CrRLK1L) families perceive RAPID ALKALINIZATION FACTOR (RALF) peptides regulate growth, reproduction, immunity, stress responses in Arabidopsis (Arabidopsis thaliana). Genes encoding these proteins are multigene most angiosperms could generate thousands variants. However, links between expansion gene functional diversification this critical as well...
In local chickens targeted for niche markets, genotyping costs are relatively high due to the small population size and diverse breeding goals. The single-step genomic best linear unbiased prediction (ssGBLUP) model, which combines pedigree information, has been introduced increase accuracy of estimated value (GEBV). Therefore, this model may be more beneficial than BLUP (GBLUP) selection in chickens. Additionally, genome-wide association study (ssGWAS) can used extend ssGBLUP results...
In this study, we demonstrated that 10′(Z), 13′(E)-heptadecadienylhydroquinone (HQ17-2), isolated from the lacquer tree, could decrease swarming motility and hemolysin activity but increase polymyxin B (PB) susceptibilityof Proteus mirabilis which is intrinsically highly-resistant to PB. The increased PB susceptibility induced by HQ17-2 was also observed in clinical isolates biofilm-grown cells. inhibit wild-type rppA mutant not rcsB mutant, indicating inhibits through RcsB-dependent...
The cis-prenyltransferase gene LLA66 (Lilium longiflorum anther-66), the first prenyltransferase to be identified in tapetum and microspores, was selected from a suppression subtractive cDNA library during microspore development anther of L. longiflorum. encodes polypeptide 308 amino acids with calculated molecular mass 35.7 kDa. Thermal asymmetric interlaced-PCR employed obtain 5′-regulatory region LLA66. Sequence alignment revealed that protein shares 30–41% identity cis-prenyltransferases...
Chronic kidney disease (CKD) patients tend to have a reduced immune response infection and vaccination. The efficacy of current available COVID-19 vaccines in CKD has not been widely evaluated.In the present study, three hundred eight chronic dialysis received ChAdOx1 nCoV-19 (Oxford-AstraZeneca, AZ). Blood tests using an antibody against receptor-binding domain (RBD) S1 subunit SARS-CoV-2 spike protein had performed at four designed time points before after first second vaccine.The mean age...