A5052266233- Antibiotic Resistance in Bacteria
- Helicobacter pylori-related gastroenterology studies
- Pneumocystis jirovecii pneumonia detection and treatment
- Antimicrobial Resistance in Staphylococcus
- Antifungal resistance and susceptibility
- Infectious Diseases and Tuberculosis
- Antibiotics Pharmacokinetics and Efficacy
- Infective Endocarditis Diagnosis and Management
- Gastroesophageal reflux and treatments
- Pneumonia and Respiratory Infections
- Amoebic Infections and Treatments
- HIV/AIDS drug development and treatment
- Mycobacterium research and diagnosis
- Fungal Infections and Studies
- Bacterial Identification and Susceptibility Testing
- Antibiotic Use and Resistance
- HIV Research and Treatment
- Orthopedic Infections and Treatments
- Clostridium difficile and Clostridium perfringens research
- HIV/AIDS Research and Interventions
- Viral Infections and Vectors
- Infections and bacterial resistance
- Eosinophilic Esophagitis
- Tuberculosis Research and Epidemiology
- Burkholderia infections and melioidosis
Kaohsiung Chang Gung Memorial Hospital
2016-2025
Chang Gung University
2016-2025
Memorial Hospital of South Bend
2004-2025
Chiayi Chang Gung Memorial Hospital
2022-2025
Chung Shan Medical University Hospital
2021
Weatherford College
2021
Chung Shan Medical University
2021
Chang Gung Memorial Hospital
2005-2020
Xiamen Chang Gung Hospital
2020
Office of Infectious Diseases
2014-2018
Neutralizing anti-interferon-γ autoantibody (nAIGA)-associated immunodeficiency is an emerging medical issue worldwide. In the present study, we describe and discuss clinical features outcomes of patients with nAIGAs disseminated infections by nontuberculous mycobacteria (dNTM). We thoroughly reviewed records all patients. Microorganisms were identified using previously described methods modifications. All data calculated analyzed SPSS software. Among 46 adult dNTM infections, 45 cases...
The first-line eradication rate of standard triple therapy for Helicobacter pylori infection has declined to <80%, and alternative therapies with >90% success rates are needed. Inconsistent were reported proton pump inhibitor- amoxicillin-containing high-dose dual therapy. We performed a prospective, randomized controlled study assess the efficacy esomeprazole- investigated influencing clinical factors. recruited 240/278 eligible H. pylori-infected patients after exclusion. They randomly...
Objectives: To investigate the safety, feasibility, and possible adverse events of single-dose human umbilical cord-derived mesenchymal stem cells in patients with moderate-to-severe acute respiratory distress syndrome. Design: Prospective phase I clinical trial. Setting: Medical center Kaohsiung, Taiwan. Patients: Moderate-to-severe syndrome a Pa o 2 /F io ratio less than 200. Interventions: Scaling for doses was required by Taiwan Food Drug Administration as follows: first three received...
BackgroundThe Delta and Omicron variants of SARS-CoV-2 are currently responsible for breakthrough infections due to waning immunity. We report phase I/II trial results UB-612, a multitope subunit vaccine containing S1-RBD-sFc protein rationally designed promiscuous peptides representing sarbecovirus conserved helper T cell cytotoxic lymphocyte epitopes on the nucleocapsid (N), membrane (M), spike (S2) proteins.MethodWe conducted I primary 2-dose (28 days apart) 10, 30, or 100 μg UB-612 in 60...
To investigate the association between Klebsiella pneumoniae-mediated invasive syndrome and underlying diseases in patients and/or K. pneumoniae characteristics, including rmpA, rmpA2, magA genes, capsular polysaccharide (cps) K(1) or K(2) serotypes, hypermucoviscosity (HV) phenotype, extended-spectrum beta-lactamase (ESBL) production.This was a prospective cross-sectional study.The detected 19 of 91 (20.9%) with infections, diabetes mellitus most common disease (9 19). The presence rmpA...
This study aimed to investigate the rate of fluconazole-non-susceptible Cryptococcus neoformans in Southern Taiwan for period 2001–2012 and analyze risk factors acquiring it among patients with invasive cryptococcosis. All enrolled strains were isolated from blood or cerebrospinal fluid samples included patients. If a patient had multiple positive results C. neoformans, only first instance was enrolled. Susceptibility testing performed using Clinical Laboratory Standards Institutes M27-A3...
A substantial number of carbapenem-resistant Gram-negative bacilli (CR GNB) have been identified among the etiologic multidrug-resistant GNB in healthcare-associated infections. For achieving a better therapeutic outcome by minimizing inappropriate empirical antibiotic treatment before blood culture and susceptibility testing results are available, it is very important to identify patients who at risk for development CR bacteremia. Retrospective analysis propensity-score matched (PSM) adult...
This study aimed to determine the predictors of persistent candidemia and examine impact biofilm formation by Candida isolates in adult patients with candidemia. Of Kaohsiung Chang Gung Memorial Hospital between January 2007 December 2012, 68 case (repeated after a 3-day systemic antifungal therapy) control non-persistent (Candida clearance from bloodstream were included based on propensity score matching for species isolated. Biofilm was assessed in-vitro using standard biomass assays....
The study aimed to characterize the genetic basis of flomoxef and collateral ertapenem resistance in a clinical isolate extended-spectrum β-lactamase-producing Klebsiella pneumoniae (ESBL-KP) after exposure. Four ESBL-KP isolates (Lkp11–14) were recovered sequentially from four episodes bacteraemia an elderly patient. Laboratory investigations included genotyping by PFGE, gene analysis PCR sequencing, outer membrane protein SDS–PAGE. Plasmid DNA–DNA hybridization, electroporation conjugation...
Better depicting the relationship between antibiotic consumption and evolutionary healthcare-associated infections (HAIs) caused by multidrug-resistant Gram-negative bacilli (MDR-GNB) may help highlight importance of stewardship.The correlations MDR-GNB HAIs at a 2,700-bed primary care tertiary referral center in Taiwan 2002 2009 were assessed. HAI referred to MDR-Enterobacteriaceae, MDR-Pseudomonas aeruginosa or MDR-Acinetobacter spp. Consumptions individual antibiotics series first...
Klebsiella pneumoniae (KP) with the hypermucoviscosity (HV) phenotype has abundant capsular polysaccharides (CPS) and usually causes an invasive syndrome. Sialic acid (Sia), a component of CPS in KP strains HV phenotype, may be anti-phagocytic. Sia-binding immunoglobulin-like lectin-9 (Siglec-9) act as MHC class-I receptor on neutrophils that recognizes Sia sends signal to dampen inflammatory response. Three clinical KP-M1 (HV-positive; serotype K1), KP-14 (HV-negative; non-K1/K2), DT-X K1)...
Two-stage reimplantation arthroplasty is one of the standard treatments for chronic periprosthetic joint infection (PJI). Scanty data exist regarding risk factors failure after two-stage hip infection. The purpose this study was to investigate and identify associated with arthroplasty.Sixty-two patients PJI treated a protocol at our institution from 2005 2012 were reviewed. Patients requiring medical treatment or reoperation recurrent defined as failure. A multivariate Cox proportional...
Although usually self-limiting, Mycoplasma pneumoniae pneumonia (MPP) may lead to clinical or radiological deterioration despite macrolide antibiotic therapy, resulting in the development of refractory MPP (RMPP). Corticosteroids have been used treat RMPP with beneficial effects. Serum lactate dehydrogenase (LDH) is a suggested biomarker for use steroid therapy. Since serum LDH non-specific marker and elevated many inflammatory processes, this study investigates predicting level isoenzymes...
We previously reported the sequential recovery of daptomycin-nonsusceptible MRSA clinical isolates with an L431F substitution in MprF protein. The aim present study is to determine effect this mutation by replacing mprF gene on chromosome a daptomycin-susceptible progenitor strain, CGK5, obtain CGK5mut having mutation. Compared daptomycin and vancomycin MICs increased from 0.5 3 μg/ml 1.5 μg/ml, respectively; however, its oxacillin MIC decreased 128 1 medium without added 2% NaCl. expression...
Abstract Severe acute respiratory syndrome (SARS) has spread to a global pandemic, especially in Asia. The transmission route of SARS been clarified, but the immunopathogenesis is unclear. In an age-matched case-control design, we studied immune parameters 15 patients who were previously healthy. Plasma was harvested for detection virus load, cytokines, and nitrite/nitrate levels, blood leukocytes subjected flow cytometric analysis intracellular mitogen-activated protein kinases (MAPKs)...
ABSTRACT Studies of macrolide resistance mutations and molecular typing using the newly proposed enhanced system for Treponema pallidum isolates obtained from HIV-infected patients in Asia-Pacific region are scarce. Between September 2009 December 2011, we conducted a survey to detect T. PCR assay clinical specimens with syphilis at six major designated hospitals HIV care Taiwan. The strains were genotyped by following methodology, which analyzed number 60-bp repeats acidic repeat protein (...
Abstract Background This study aimed to investigate the correlation of minimum inhibiting concentrations (MICs), obtained by broth micro-dilution, and clinical response in patients with cryptococcal meningitis. Methods Using retrospective analyses covering period 2001–2010, factors affecting therapeutic cure meningitis 10 weeks after start anti-fungal therapy were identified. Specific emphasis was placed on role susceptibility. Results Of 46 identified, 21 cured treatment. Overall, 12...
Background Ventilator-associated pneumonia (VAP) caused by Acinetobacter baumannii is increasing. It has a high mortality rate but experience in using inhaled colistin as monotherapy for VAP children, especially pre-term infants, limited. This study presents experiences aerosolized due to A. infection infants. Methods Eight infants (gestational age 25–36 weeks) admitted the neonatal intensive care unit (NICU) of Kaohsiung Chang Gung Memorial Hospital Taiwan from January 2006 December 2010...
Klebsiella pneumoniae (KP), with production of abundant capsular polysaccharide (CPS), is capable causing invasive syndrome. Environmental glucose stimuli may increase CPS biosynthesis. We aimed to investigate the relationship between glycemic control and KP-mediated syndrome in diabetic patients effect on Diabetic community-acquired KP bacteremia were included study risk factors KP-M1, a serotype-K1 clinical isolate, was used examine biosynthesis cps gene expression, exogenous bacterial...