A5013007008- Xenotransplantation and immune response
- Virus-based gene therapy research
- Animal Genetics and Reproduction
- Cancer-related Molecular Pathways
- Inflammatory Bowel Disease
- CRISPR and Genetic Engineering
- Historical and Linguistic Studies
- Religion, Theology, and Education
- Mycobacterium research and diagnosis
- Cancer Genomics and Diagnostics
- Law and Political Science
- Biblical Studies and Interpretation
- IL-33, ST2, and ILC Pathways
- Pluripotent Stem Cells Research
- Renal and related cancers
- Gut microbiota and health
- German Literature and Culture Studies
- Tissue Engineering and Regenerative Medicine
- Genetic factors in colorectal cancer
- Viral gastroenteritis research and epidemiology
- Helicobacter pylori-related gastroenterology studies
- Colorectal Cancer Treatments and Studies
- Sarcoma Diagnosis and Treatment
- Monoclonal and Polyclonal Antibodies Research
- Advanced Biosensing Techniques and Applications
Technical University of Munich
2019-2023
Weihenstephan-Triesdorf University of Applied Sciences
2019
University of Zurich
1997
Abstract Background Cell surface carbohydrate antigens play a major role in the rejection of porcine xenografts. The most important for human recipients are α‐1,3 Gal (Galactose‐alpha‐1,3‐galactose) causing hyperacute rejection, also Neu5Gc (N‐glycolylneuraminic acid) and Sd(a) blood group both which likely to elicit acute vascular given known immune status. Porcine cells with knockouts three genes responsible, GGTA1 , CMAH B4GALNT2 revealed minimal xenoreactive antibody binding after...
Using a cell-bound immunogen, we have generated monoclonal antibody, 3D5, that recognizes carboxy-terminal oligo-histidine tags (His tags) on wide variety of proteins. From this single-chain fragment the variable domains (scFv), dimeric scFv-alkaline phosphatase fusion and an oligovalent scFv-display phage. The antibody in its various formats is effective tool used fluorescence-activated cell sorting analysis, BIAcore method, Western blots enzyme-linked immunosorbent assay (ELISA). ELISAs...
Abstract Background and Aims Crohn’s disease [CD] is a major subtype of inflammatory bowel diseases [IBD] with increasing incidence prevalence. Results studies using available small large animal models are often poorly translatable to patients, few CD show intestinal pathology. Due its similarities humans, the pig has emerged as highly suitable translational model, particularly for testing novel nutritional technological interventions. Our goal was develop physiologically relevant porcine...
Abstract Background Porcine cytomegalovirus (PCMV) is a porcine roseolovirus (PCMV/PRV) which widely distributed in pigs. Transmission of PCMV/PRV preclinical xenotransplantations was shown to significantly reduce the survival time pig transplants non-human primates. also transmitted first transplantation heart into human patient. To analyze how could be introduced breeds, especially considering cloned transgenic pigs, and subsequently spread breeding facilities, we screened ovaries derived...
Abstract Recent years have seen an increasing number of genetically engineered pig models human diseases including cancer. We previously generated pigs with a modified TP53 allele that carries Cre-removable transcriptional stop signal in intron 1, and oncogenic mutation R167H (orthologous to R175H ) exon 5. Pigs the unrecombined mutant (fl develop mainly osteosarcoma but also nephroblastomas lymphomas. This observation suggested gene dysfunction is itself key initiator bone tumorigenesis,...
Germline mutations in the adenomatous polyposis coli (APC) gene are responsible for familial (FAP). FAP patients develop multiple colonic adenomas early life. The number of polyps can vary considerably because different mutations1Nugent K.P. et al.Gut. 1994; 35: 1622-1623Crossref PubMed Scopus (119) Google Scholar but also family members carrying same mutation.2Crabtree M.D. 2002; 51: 420-423Crossref (69) Several genes have been considered as modifiers intrafamilial severity...
Abstract Background: Porcine cytomegalovirus (PCMV) is a porcine roseolovirus (PCMV/PRV) which widely distributed in pigs. Transmission of PCMV/PRV preclinical xenotransplantations was shown to significantly reduce the survival time pig transplants non-human primates. also transmitted first transplantation heart into human patient. To analyze how could be introduced breeds, especially considering cloned transgenic pigs, and subsequently spread breeding facilities, we screened ovaries derived...
Abstract Background The pig has long been an important animal species for biomedical research. Recent years also seen increasing number of genetically engineered models human diseases including cancer. We previously generated pigs with a modified TP53 allele which carries Cre-removable transcriptional stop signal in intron 1, and oncogenic mutation R167H (orthologous to R175H mouse Trp53 R172H ) exon 5. Pigs the unrecombined mutant (fl develop osteosarcoma (OS) aged heterozygous young...
Abstract Crohn’s Disease (CD) is incurable, and represents a lifelong burden for patients its incidence increasing worldwide. A key contributing factor dysregulated immune response. Here we report the generation of genome edited pigs with deletion transcript-destabilizing AU-rich element (ARE) constitutive decay (CDE) in TNF gene which recapitulate major characteristics human CD, including ulcerative transmural ileocolitis, increased abundance proinflammatory cytokines, evidence impaired...