A5100760299- Cancer-related molecular mechanisms research
- Circular RNAs in diseases
- RNA modifications and cancer
- MicroRNA in disease regulation
- Cancer Immunotherapy and Biomarkers
- Trace Elements in Health
- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- Molecular Biology Techniques and Applications
First Affiliated Hospital of Zhengzhou University
2018-2025
Background: Schwann cells (SCs) can potentially transform into the repair-related cell phenotype after injury, which promote nerve repair. Ferroptosis occurs in SCs of injured tissues, causing damage to...
Long non-coding RNAs have the regulatory roles in different kinds of human cancers. The key point this study was to research functional mechanisms urothelial carcinoma associated 1 (UCA1) development osteosarcoma. Quantitative real-time PCR adopted for expression detection UCA1, microRNA-513b-5p (miR-513b-5p) and E2F transcription factor 5 (E2F5). target relation verified via dual-luciferase reporter assay RNA pull-down assay. Cell proliferation evaluated using Counting Kit-8 colony...
Cancer immunotherapy is a promising therapeutic approach, but the prognostic value of immune-related genes in osteosarcoma (OS) unknown. Here, Target-OS RNA-seq data were analyzed to detect differentially expressed (DEGs) between OS subgroups, followed by functional enrichment analysis. Cox proportional risk regression was performed for each gene, and score model predict prognosis patients with constructed. The scores calculated using signature divide training set into high-risk low-risk...
Aim: This study aims to investigate whether long noncoding RNA (lncRNA) X-inactive specific transcript (Xist) can regulate osteoclast differentiation in osteoporosis and the mechanism. Materials & methods: The mouse model of was established by ovariectomy surgery. Osteoclast from RAW264.7 cells induced vitro. relationships between associated genes were assessed. Results:Xist Tgif2 upregulated, but miR-590-3p downregulated femurs cell models. Xist knockdown or overexpression inhibited...
Objective To explore the inhibitory effect of small interfering RNA(siRNA) on Cathepsin K expression and growth invasion ability osteosarcoma cells. Methods A mRNA- targeting siRNA was transfected into MG63 cells with lipidosme. Reverse transcriptasepolymerase chain reaction(RT- PCR)and Western blotting were used to detect mRNA protein respectively. Methyl thiazol tetrazolium(MTT)and Transwell assays applied measure proliferation cells. Results The levels in siRNA(0.05± 0.16...