A5017194144- Plant Stress Responses and Tolerance
- Plant Molecular Biology Research
- Seed Germination and Physiology
- Plant Gene Expression Analysis
- Plant responses to water stress
- RNA Research and Splicing
- Photosynthetic Processes and Mechanisms
- Polysaccharides and Plant Cell Walls
- Autophagy in Disease and Therapy
- Allelopathy and phytotoxic interactions
- Plant nutrient uptake and metabolism
- Microbial bioremediation and biosurfactants
- Single-cell and spatial transcriptomics
- Plant Genetic and Mutation Studies
- Neutropenia and Cancer Infections
- Cancer therapeutics and mechanisms
- Chronic Lymphocytic Leukemia Research
- Plant responses to elevated CO2
- Tumors and Oncological Cases
- Plant Water Relations and Carbon Dynamics
- Forest ecology and management
- Genomic variations and chromosomal abnormalities
- Redox biology and oxidative stress
- Botany and Plant Ecology Studies
- Epigenetics and DNA Methylation
Adam Mickiewicz University in Poznań
2015-2024
Cook Children's Medical Center
2024
Brigham and Women's Hospital
2021-2022
Wroclaw Medical University
2022
Institute of Dendrology
2019-2022
Polish Academy of Sciences
2019-2022
Harvard University
2021
Nicolaus Copernicus University
2013-2015
The formation of multifunctional vascular tissues represents a significant advancement in plant evolution. Differentiation conductive cells is specific, involving two main pathways, namely protoplast clearance and cell wall modification. In xylogenesis, autophagy crucial process for complete elimination tracheary elements, whose also undergoes strong changes. Knowledge pertaining to living sieve which lose most their during phloemogenesis, remains limited. We hypothesized that plays role,...
Senescence, despite its destructive character, is a process that precisely-regulated. The control of senescence required to achieve remobilization resources, principle aspect senescence. Remobilization allows plants recapture valuable resources would otherwise be lost the environment with senescing organ. Autophagy one critical processes switched on during This evolutionarily conserved plays dual, antagonistic roles. On hand, it counteracts instantaneous cell death and set in motion, while...
Autophagy is involved in developmentally programmed cell death and identified during the early development of phloem, as well xylem with a dual role, both an inducer executioner death. The regulation primary secondary roots stems important for establishment root systems overall survival trees. molecular cellular basis autophagic processes, which are used at distinct moments growth organs, crucial to understand their development. To address this, we use Populus trichocarpa seedlings grown...
Germline missense variants of unknown significance in cancer-related genes are increasingly being identified with the expanding use next-generation sequencing. The ataxia telangiectasia-mutated (
Abstract The remobilization and resorption of plant nutrients is considered as a crucial aspect the seasonal senescence organs. In leaves, mechanisms responsible for relocation valuable compounds are well understood while related processes in roots still being debated. Some research indicates that occurs, other studies have not found evidence this process. Considering total biomass fine equal to or greater than clarifying conflicting reports ambiguities may provide critical information on...
Plant senescence is a highly regulated process that allows nutrients to be mobilized from dying tissues other organs. Despite has been extensively studied in leaves, the of ephemeral organs located underground still poorly understood, especially context phytohormone engagement. The present study focused on filling this knowledge gap by examining roles abscisic acid (ABA) and jasmonate regulation fine, absorptive roots leaves Populus trichocarpa. Immunohistochemical (IHC), chromatographic,...
Small nuclear ribonucleoproteins (snRNPs) play a crucial role in pre-mRNA splicing all eukaryotic cells. In contrast to the relatively broad knowledge on snRNP assembly within nucleus, spatial organization of cytoplasmic stages their maturation remains poorly understood. Nevertheless, sparse research indicates that, similar steps, processes may also be strictly spatially regulated. European larch microsporocytes, it was determined that snRNPs cell might occur two distinct manners, which...
Regulation of gene expression, as determined by the genetics tree species, is a major factor in determining wood quality. Therefore, identification genes that play role xylogenesis extremely important for understanding mechanisms shaping plant phenotype. Efforts to develop new varieties characterized higher yield and better quality will greatly benefit from recognizing complex transcriptional network underlying development. The present study provides detailed comparative description changes...
Abstract Desiccation tolerance is a developmental program enabling seed survival in dry state and common seeds categorized as orthodox. We focused on NAD its phosphorylated form (NADP) because their continual switching between reduced (NAD(P)H) oxidized (NAD(P)+) forms involved the modulation of redox signaling determination reducing power further antioxidant responses. Norway maple sycamore representing orthodox recalcitrant categories, respectively, were used models comparison responses to...
Norway maple and sycamore produce desiccation-tolerant (orthodox) desiccation-sensitive (recalcitrant) seeds, respectively. Drying affects reduction oxidation (redox) status in seeds. Oxidation of methionine to sulfoxide (MetO) via reductases (Msrs) have never been investigated relation seed desiccation tolerance. MetO levels the abundance Msrs were reactive oxygen species (ROS) such as hydrogen peroxide, superoxide anion radical hydroxyl (•OH), ascorbate glutathione redox couples gradually...
Two related tree species, Norway maple (Acer platanoides L.) and sycamore pseudoplatanus L.), produce desiccation-tolerant (orthodox) desiccation-sensitive (recalcitrant) seeds, respectively. We compared the seeds of these two species to characterize developmentally driven changes in levels peptide-bound methionine sulfoxide (MetO) abundance reductases (Msrs) B1 B2, with respect cellular redox environment. Protein oxidation at Met level was dynamic only reduced MsrB2 form detected this...
In contrast to aboveground organs (stems and leaves), developmental events their regulation in underground organs, such as pioneer fine roots, are quite poorly understood. The objective of the current study was achieve a better understanding physiological molecular role reactive oxygen species (ROS) ROS-related enzymes process stem root development black cottonwood (Populus trichocarpa), well senescence leaves roots. Results transcriptomic analysis revealed that primary/secondary growth...
European beech is an important component of lowland forests in terms ecology, and produces irregular seeds categorized as intermediate due to their limited longevity. Removal the excess reactive oxygen species crucial for redox homeostasis growing plant tissues. Hydrogen peroxide (H2O2) detoxified via plant-specific ascorbate-glutathione cycle, enzymatically, mainly by catalase (CAT). The reduced oxidized (redox) forms ascorbate (AsA, DHA) glutathione (GSH, GSSG) decreased during maturation...
Seed germination is a complex process enabling plant reproduction. Germination was found to be regulated at the proteome, metabolome and hormonal levels as well via discrete post-translational modification of proteins including phosphorylation carbonylation. Redox balance also involved but less studied. Acer seeds displaying orthodox recalcitrant characteristics were investigated determine redox couples nicotinamide adenine dinucleotide (NAD) phosphate (NADP) integrated with ascorbate...
The levels of methionine sulfoxide (MetO) and the abundances reductases (Msrs) were reported as important for desiccation tolerance Acer seeds. To determine whether MetO/Msrs system is related to reactive oxygen species (ROS) involved in regulation germination orthodox recalcitrant seeds, Norway maple sycamore investigated. Changes water content, MetO abundance MsrB1 MsrB2 relation ROS content activity depending on nicotinamide adenine dinucleotides monitored. seeds differed...
Norway maple and sycamore, two Acer genus species, have an important ecological value different sensitivity to stressing factors being currently aggravated by climate change. Seedling growth is postulated be the main barrier for successful plant establishment under change scenarios. Therefore, differences in redox regulation during seedling performance of sycamore were investigated. Seeds species exhibited identical high germination capacity, whereas emergence was higher sycamores. PCA...
Immunotherapy has emerged as a promising treatment approach in oncology, it is specifically designed to boost the strength and accuracy of immune system, allowing target tumor cells but spare non-tumor tissue. This not only demonstrates potential for improved clinical outcomes may also be associated with fewer adverse effects compared traditional therapies. Despite its early success, application immunotherapy largely been limited adult cancer patients, slow adoption noted pediatric patients....
Abstract Background and Aims The transformation of sieve elements from meristematic cells, equipped with a full complement organelles, to specialized transport tubes devoid nucleus has long been enigmatic. We hypothesized strong involvement various degradation pathways, particularly macroautophagy in this context, emphasizing the importance autophagic selectivity remaining viability these cells. Methods Experiments were performed on pioneer roots Populus trichocarpa cultivated rhizotrons...
Beech seeds are produced irregularly, and there is a need for long-term storage of these forest management practices. Accumulated reactive oxygen species broadly oxidize molecules, including amino acids, such as methionine, thereby contributing to decreased seed viability. Methionine oxidation can be reversed by the activity methionine sulfoxide reductases (Msrs), which enzymes involved in regulation many developmental processes stress responses. Two types Msrs, MsrB1 MsrB2, were...