A5000755381- Pluripotent Stem Cells Research
- 3D Printing in Biomedical Research
- Tissue Engineering and Regenerative Medicine
- Developmental Biology and Gene Regulation
- Nerve injury and regeneration
- Planarian Biology and Electrostimulation
- Cancer Cells and Metastasis
- Cellular Mechanics and Interactions
- Congenital heart defects research
- MicroRNA in disease regulation
- Music Education and Analysis
- Cultural and Sociopolitical Studies
- Nanofabrication and Lithography Techniques
- Turkish Urban and Social Issues
- Education Practices and Challenges
- Diverse Music Education Insights
- Islamic Thought and Society Studies
- Molecular Biology Techniques and Applications
- Lexicography and Language Studies
- Turkish Literature and Culture
- Neuroscience and Neural Engineering
- Innovative Microfluidic and Catalytic Techniques Innovation
- Single-cell and spatial transcriptomics
- Nuclear Receptors and Signaling
Roche (Switzerland)
2023
École Polytechnique Fédérale de Lausanne
2016-2021
University of Basel
2021
Institute of Cellular and Organismic Biology, Academia Sinica
2019
Bioengineering Center
2019
Institute of Environmental Studies & Wetland Management
2019
Three-dimensional organoid constructs serve as increasingly widespread in vitro models for development and disease modeling. Current approaches to recreate morphogenetic processes rely on poorly controllable ill-defined matrices, thereby largely overlooking the contribution of biochemical biophysical extracellular matrix (ECM) factors promoting multicellular growth reorganization. Here, we show how defined synthetic matrices can be used explore role ECM complex 3D neuroepithelial cysts that...
When stimulated with a pulse from an exogenous WNT pathway activator, small aggregates of mouse embryonic stem cells (ESCs) can undergo embryo-like axial morphogenesis and patterning along the three major body axes. However, these structures, called gastruloids, currently lack anterior regions, such as those belonging to brain. Here, we describe approach generate gastruloids that have more complete antero-posterior development. We used hydrogel microwell arrays promote robust derivation ESCs...
The difficulty of studying post-implantation development in mammals has sparked a flurry activity to develop vitro models, termed embryoids, based on self-organizing pluripotent stem cells. Previous approaches derive embryoids either lack the physiological morphology and signaling interactions, or are unconducive model post-gastrulation development. Here, we report bioengineering-inspired approach aimed at addressing this gap. We employ high-throughput cell aggregation simultaneously coax...
Abstract Organoids are powerful models for studying tissue development, physiology, and disease. However, current culture systems disrupt the inductive tissue-tissue interactions needed complex morphogenetic processes of native organogenesis. Here we show that mouse embryonic stem cells (mESCs) can be coaxed to robustly undergo fundamental steps early heart organogenesis with an in vivo -like spatiotemporal fidelity. These axially patterned organoids support generation cardiovascular...
Abstract When stimulated with a pulse from an exogenous WNT pathway activator, small aggregates of mouse embryonic stem cells (ESCs) can undergo embryo-like axial morphogenesis and patterning along the three major body axes. However, these structures, called gastruloids, currently lack anterior regions, such as those belonging to brain. Here we describe approach generate gastruloids that have more complete antero-posterior development. We used bioengineered hydrogel microwell arrays promote...
Embryonic development is a complex process integrating cell fate decisions and morphogenesis in spatiotemporally controlled manner. Previous studies with model organisms laid the foundation of our knowledge on post-implantation development; however, studying mammalian embryos at this stage difficult laborious process. Early attempts to recapitulate vitro begun embryoid bodies (EBs), which aggregates mouse embryonic stem cells (mESCs) were shown differentiate into spatially arranged germ...
Methods for screening combinations of signals their effects on stem cell behavior are needed in the field tissue engineering. We introduce a microgel-based platform testing proteins fate.
Abstract Establishment of the three body axes is a critical step during animal development. In mammals, genetic studies have shown that combination precisely deployed signals from extraembryonic tissues position anteroposterior axis (AP) within embryo and lead to emergence dorsoventral (DV) left-right (LR) axes. We used Gastruloids , embryonic organoids, as model system understand this process find they are able develop AP, DV LR well undergo axial elongation in manner mirror embryos. The...
Human mammary epithelial cells can proliferate and reorganize into polarized multi-cellular constructs in-vitro, thereby functioning as an important model system in recapitulating key steps of in-vivo morphogenesis. Current approaches to constructing such three-dimensional mimics the microenvironment have involved use complex ill-defined naturally derived matrices, whose properties are difficult manipulate independently, which therefore limited our ability understand extrinsic regulation...
The difficulty of studying post-implantation development in mammals has sparked a flurry activity to develop vitro models, termed embryoids, based on self-organizing pluripotent stem cells. Previous approaches derive embryoids either lack the physiological morphology and signaling interactions, or are not yet optimal for modeling post-gastrulation development. Here, we report bioengineering-inspired approach aimed at addressing this gap. A high-throughput cell aggregation was employed...
Standard protocol used for immunostaining Gastruloids (aggregates of mouse embryonic stem cells) in the Lutolf Lab, EPFL. For a published on how to generate Gastruloids, see:
Gastruloid generation protocol as performed in the Lutolf Lab, EPFL. For previously published protocols, see: Baillie-Johnson, Peter, et al. "Generation of aggregates mouse embryonic stem cells that show symmetry breaking, polarization and emergent collective behaviour vitro."JoVE (Journal Visualized Experiments)105 (2015): e53252. Mehmet, G., "Generating Gastruloids from Mouse Embryonic Stem Cells."Protocol Exchange (2018).
Standard protocol used for culturing mouse embryonic stem cells (mESCs) in the Lutolf Lab, EPFL.