A5032982134- 3D Printing in Biomedical Research
- Pluripotent Stem Cells Research
- Liver physiology and pathology
- Mesenchymal stem cell research
- Tissue Engineering and Regenerative Medicine
- Genetic Mapping and Diversity in Plants and Animals
- Wheat and Barley Genetics and Pathology
- Neurogenesis and neuroplasticity mechanisms
- Innovative Microfluidic and Catalytic Techniques Innovation
- Graphene and Nanomaterials Applications
- Nanoparticles: synthesis and applications
- Genetics and Plant Breeding
- High Entropy Alloys Studies
- Natural Fiber Reinforced Composites
- Renal and related cancers
- Mechanical Engineering and Vibrations Research
- Microfluidic and Bio-sensing Technologies
- Cancer Cells and Metastasis
- Advanced biosensing and bioanalysis techniques
- Nanoparticle-Based Drug Delivery
- Luminescence and Fluorescent Materials
- Cystic Fibrosis Research Advances
- Nanotechnology research and applications
- Adipose Tissue and Metabolism
- Biosensors and Analytical Detection
University of North Texas
2021-2024
University of California, San Francisco
2024
Vellore Institute of Technology University
1969-2023
Saveetha University
2020-2021
Molecular Theranostics (United States)
2019
Sri Venkateswara University
2016
Indian Institute of Technology Kharagpur
2014
Washington State University
2006
Beaumont Hospital, Royal Oak
1997
Kane Biotech (Canada)
1995
The insulin-dependent tyrosine kinase activity (TKA) of the insulin receptor (IR) plays an essential role in signaling. Thus, dysregulation IR-TKA might be important element states resistance. A phosphorylated rat hepatic glycoprotein (pp63) acting as inhibitor IR-TK has been described. In search human homolog pp63, we isolated a cDNA clone from liver lambda gt11 library. DNA sequence analysis reveals identity with mRNA product gene AHSG encoding serum protein, alpha 2-Heremans...
We developed microarray 3D bioprinting technology and associated pillar/deep well plates that allow high-throughput, miniature, uniform culture of human liver organoids (HLOs) from bioprinted foregut cells facilitate in situ assessments.
Static three-dimensional (3D) cell culture has been demonstrated in ultralow attachment well plates, hanging droplet and microtiter plates with hydrogels or magnetic nanoparticles. Although it is simple, reproducible, relatively inexpensive, thus potentially used for high-throughput screening, statically cultured 3D cells often suffer from a necrotic core due to limited nutrient oxygen diffusion waste removal have vivo-like tissue structure. Here, we overcome these challenges by developing...
Bioimplants are susceptible to simultaneous wear and corrosion degradation in the aggressive physiological environment. High entropy alloys with equimolar proportion of constituent elements represent a unique alloy design strategy for developing bioimplants due their attractive mechanical properties, superior wear, resistance. In this study, tribo-corrosion behavior an equiatomic MoNbTaTiZr high consisting all biocompatible was evaluated compared 304 stainless steel as benchmark. The showed...
Ceria nanoparticles (CeO2 NPs) are generally considered in various functional applications, such as catalysts fuel cells, sensors, and antioxidant oxidase-like enzymes the biological environment. The CeO2 NPs were synthesized using E. globulus leaf extract-mediated hydrothermal technique. characterized by analytical instruments including powder X-ray diffractometer (PXRD), scanning electron microscope (SEM), transmission (TEM) dynamic light scattering (DLS) analysis. XRD results showed an...
This study mainly deals with an effective one-pot solvothermal synthetic pathway for the preparation of uniformly dispersed zirconium oxide nanoparticles on flattened rough surface reduced graphene (ZrO2/rGO NCs) using aqueous leaf extract Andrographis paniculata. After obtaining detailed information and characterization, anticancer activity synthesized ZrO2/rGO nanocrystals (NCs) was evaluated two human cancer cell lines (A549 HCT116) along one normal line (hMSC). The...
The leaching out of toxic elements from metallic bioimplants has serious repercussions, including allergies, peripheral neuritis, cancer, and Alzheimer's disease, leading to revision or replacement surgeries. development advanced structural materials with excellent biocompatibility superior corrosion resistance in the physiological environment holds great significance. High entropy alloys (HEAs) a huge compositional design space outstanding mechanical functional properties can be promising...
Abstract Despite the potential toxicity of commercial chemicals to development nervous system (known as developmental neurotoxicity or DNT), conventional in vitro cell models have primarily been employed for assessment acute neuronal toxicity. On other hand, animal used DNT are not physiologically relevant due heterogenic difference between humans and animals. In addition, low-throughput, time-consuming, expensive, ethically questionable. Recently, human brain organoids emerged a promising...
Abstract Human liver organoids (HLOs) differentiated from embryonic stem cells (ESCs), induced pluripotent (iPSCs), and adult (ASCs) can recapitulate the structure function of human fetal tissues, thus being considered as a promising tissue model for diseases predictive compound screening. However, adoption HLOs in drug discovery faces several technical challenges, which include lengthy differentiation process with multiple culture media leading to batch-to-batch variation, short-term...
Abstract Despite the potential toxicity of commercial chemicals to development nervous system (known as developmental neurotoxicity or DNT), conventional in vitro cell models have primarily been employed for assessment acute neuronal toxicity. On other hand, animal used DNT are not physiologically relevant due heterogenic difference between humans and animals. In addition, low-throughput, time-consuming, expensive, ethically questionable. Recently, human brain organoids emerged a promising...
Although neural stem cells (NSCs) have potential applications in treating neurological disorders, much still needs to be understood about the differentiation biology for their successful clinical translation. In this study, we aimed at deriving NSCs from human umbilical cord blood-derived mesenchymal (hUCB-MSCs) and explored role of Notch signaling process. The hUCB-MSCs were characterized as per guidelines International Society Cellular Therapy. successfully generated by using epidermal...
Light-responsive fluorescent organic nanoparticles (NPs) based on tetraphenylethylene (TPE) have been developed for the release of 4 equiv chlorambucil (Cbl) and photodynamic therapy (PDT) utilizing aggregation-induced-emission (AIE) phenomena. Our newly TPE(Cbl)4 NPs displayed strong fluorescence because its AIE. Upon exposure to visible light, our simultaneously produced singlet oxygen uncaged anticancer drug in a sequential manner their aggregated state. On other hand, released...
Abstract Static three-dimensional (3D) cell culture has been demonstrated in ultralow attachment well plates, hanging droplet and microtiter plates with hydrogels or magnetic nanoparticles. Although it is simple, reproducible, relatively inexpensive, thus potentially used for high-throughput screening, statically cultured 3D cells often suffer from the necrotic core due to limited nutrient oxygen diffusion waste removal have vivo -like tissue structure. Here, we overcome these challenges by...
Abstract Human liver organoids (HLOs) hold significant potential for recapitulating the architecture and function of tissues in vivo. However, conventional culture methods HLOs, forming Matrigel domes 6-/24-well plates, have technical limitations such as high cost low throughput organoid-based assays predictive assessment compounds clinical pharmacological lab settings. To address these issues, we developed a unique microarray 3D bioprinting protocol progenitor cells biomimetic hydrogels on...
Hydrogen sulfide (H<sub>2</sub>S) behaves like a two-edged sword, at low concentrations it has beneficial and cytoprotective effects, while higher exhibits toxicity.
Acute acetaminophen (APAP) toxicity is a predominant clinical problem, which causes serious liver injury in both humans and experimental animals. This study presents the histological biochemical factor antioxidant enzyme level changes induced by an acute overdose Wistar albino rat livers to elucidate effective hepatoprotective potential of biofabricated palladium nanoparticle-decorated reduced graphene oxide nanocomposites (rGO/PdNPs-NC) compared silymarin. After detailed characterization...
Carbon nanotubes have emerged as potential reinforcements that can be used to engineer composites with tailored combinations of physical and mechanical properties. In the present study, an Al alloy-based nanostructured composite containing multiwalled carbon (MWCNT) a reinforcement phase has been synthesized by combination two novel processing techniques, which are cryomilling spark plasma sintering. Scanning electron microscopy quantitative image analysis performed provide insight into...
Liver tissues, composed of hepatocytes, cholangiocytes, stellate cells, Kupffer and sinusoidal endothelial are differentiated from endodermal mesodermal germ layers. By mimicking the developmental process liver, various differentiation protocols have been published to generate human liver organoids (HLOs) in vitro using induced pluripotent stem cells (iPSCs). However, HLOs derived solely layer often encounter technical hurdles such as insufficient maturity functionality, limiting their...