A5036579970- Neuroinflammation and Neurodegeneration Mechanisms
- Neurogenesis and neuroplasticity mechanisms
- Cerebrospinal fluid and hydrocephalus
- Immune Response and Inflammation
- Wnt/β-catenin signaling in development and cancer
- Neuroscience and Neuropharmacology Research
- Neonatal and fetal brain pathology
- Retinoids in leukemia and cellular processes
- Axon Guidance and Neuronal Signaling
- Cannabis and Cannabinoid Research
- Tryptophan and brain disorders
- Neurobiology and Insect Physiology Research
- Antioxidant Activity and Oxidative Stress
- Developmental Biology and Gene Regulation
- Estrogen and related hormone effects
- Glycosylation and Glycoproteins Research
- Craniofacial Disorders and Treatments
- Neuropeptides and Animal Physiology
- Aquaculture Nutrition and Growth
- Cell Adhesion Molecules Research
- Neonatal Respiratory Health Research
- Immune cells in cancer
- Alzheimer's disease research and treatments
- Chemokine receptors and signaling
- Regulation of Appetite and Obesity
Universidad de Málaga
2014-2024
Instituto de Investigación Biomédica de Málaga
2014-2024
Andalusian Centre for Nanomedicine and Biotechnology
2017-2024
Collège de France
1996-1997
Inserm
1996-1997
Centre National de la Recherche Scientifique
1996-1997
Laboratoire de Biologie Moléculaire et Cellulaire des Eucaryotes
1994
Institut de Biologie Structurale
1994
ABSTRACT We describe here the analysis of congenital malformations in compound mutant fetuses bearing null alleles one RXR (α β or γ) and RAR isotype gene. A marked synergy was observed between effects mutations RXRα RARs, as a large number developmental defects previously found mainly single mutants were recapitulated specific RXRα/RAR mutants. Several seen only type combination, whereas others several types double No either RXRβ RXRγ those any mutations. These genetic data suggest that...
It is known that microglia morphology and function are closely related, but only few studies have objectively described different morphological subtypes. To address this issue, parameters of microglial cells were analyzed in a rat model aseptic neuroinflammation. After the injection single dose enzyme neuraminidase (NA) within lateral ventricle (LV) an acute inflammatory process occurs. Sections from NA-injected animals sham controls immunolabeled with marker IBA1, which highlights...
We have generated mouse lines in which the RXR beta gene was disrupted by homologous recombination. Approximately 50% of homozygous mutants died before or at birth, but those that survived appeared normal except males were sterile, owing to oligo-astheno-teratozoospermia. Failure spermatid release occurred within germinal epithelium, and epididymis contained very few spermatozoa that, addition, exhibited abnormal acrosomes tails. There a progressive accumulation lipids mutant Sertoli cells,...
Abstract In the brain of adult rats neurogenesis persists in subventricular zone lateral ventricles and dentate gyrus hippocampus. By contrast, low proliferative activity was observed hypothalamus. We report here that, after intracerebroventricular treatment with insulin‐like growth factor I (IGF‐I), cell proliferation significantly increased both periventricular parenchymal zones whole Neurons, astrocytes, tanycytes, microglia endothelial cells local vessels were stained marker...
Microglia are the resident macrophages in brain. Traditionally, two forms of microglia have been described: one considered as a resting/surveillant state which cells highly branched morphology, and another an activated they acquire de-ramified or amoeboid form. However, many studies describe intermediate microglial morphologies emerge during pathological processes. Since form function closely related, it is interest to correlate morphology with extent its activation. To address this issue,...
ABSTRACT Knock-out of the mouse RXRa gene was previously shown to result in a hypoplastic heart ventricular wall, histologically detectable 12.5 dpc fetuses. We show here that precocious differentiation can be detected as early 8.5 cardiomyocytes RXRα−/− mutants. This differentiation, which is characterized by presence striated myofibrils, sarcoplasmic reticulum and intercalated disks, found after 9.5 about 50% subepicardial myocytes. In contrast, wild-type myocytes remain morphologically...
ABSTRACT The eye is the organ whose development most frequently altered in response to maternal vitamin A deficiency [VAD; Warkany, J. and Schraffenberger, S. (1946). Archs Ophthalmol. 35, 150-169]. With exception of prenatal retinal dysplasia, all ocular abnormalities fetal VAD syndrome are recapitulated mouse mutants lacking either RARα RARβ2, RARγ? RARγ or RXRα [Lohnes, D., Mark, M., Mendelsohn, C., Dollé, P., Dierich, A., Gorry, Gansmuller, A. Chambon, P. (1994) Development 120,...
Soy extracts have been claimed to be neuroprotective against brain insults, an effect related the estrogenic properties of isoflavones. However, effects individual isoflavones on obesity-induced disruption adult neurogenesis not yet analyzed. In present study we explore pharmacological administration daidzein, a main soy isoflavone, in cell proliferation, apoptosis and gliosis hippocampus animals exposed very high-fat diet. Rats made obese after 12-week exposure standard or (HFD, 60%) diets...
Abstract Short-term behavioral alterations are associated with infection and aid the recovery from sickness. However, concerns have raised that sustained disturbances after acute neuroinflammation could relate to neurological diseases in long run. We aimed explore medium- long-term rats, using a model based on intracerebroventricular administration of enzyme neuraminidase (NA), which is part some pathogenic bacteria viruses. Neurological assessments were performed 2 10 weeks injection NA,...
Neuraminidase (NA) is a sialidase present, among various locations, in the envelope/membrane of some bacteria/viruses (e.g., influenza virus), and involved infectiveness and/or dispersion. The administration NA within brain lateral ventricle represents model acute sterile inflammation. relevance Toll-like receptors TLR2 TLR4 (particularly those microglial cells) such process was investigated.Mouse strains deficient either (TLR2-/-) or (TLR4-/-) were used. injected ventricle, inflammatory...
Echinoderms and chordates belong to the same monophyletic taxon, Deuterostomia. In spite of significant differences in body plan organization, two phyla may share more common traits than was thought previously. Of particular interest are features organization central nervous system. The present study employs polyclonal antisera raised against bovine Reissner's substance (RS), a secretory product produced by glial cells subcomissural organ, RS-like immunoreactivity system sea cucumbers....
Exposure of microglia to an inflammatory environment may lead their priming and exacerbated response future stimuli. Here we aimed explore hypothalamic its consequences on energy balance regulation. A model intracerebroventricular administration neuraminidase (NA, which is present in various pathogens such as influenza virus) was used induce acute neuroinflammation. Evidences primed were observed 3 months after NA injection, namely (1) a heightened located the arcuate nucleus vivo challenge...
Endocannabinoid signalling participates in the control of neurogenesis, especially after brain insults. Obesity may explain alterations physiology affecting although it is unclear whether cannabinoid modulate neural proliferation obese animals. Here we analyse impact obesity by using two approaches, a high-fat diet (HFD, 60% fat) and standard/low-fat (STD, 10% fat), response to subchronic treatment with receptor type 1 (CB1) inverse agonist AM251 (3 mg/kg) on cell relevant neurogenic...
To investigate the role of sialic acid in ependyma rat brain, we injected neuraminidase from Clostridium perfingens into lateral ventricle 86 adult rats that were sacrificed at various time intervals. After administration 10 μg neuraminidase, ciliated cuboidal ependymal cells ventricles, third ventricle, cerebral aqueduct, and rostral half fourth died detached. The regions sealed by tight juntions such as choroid plexus subcommissural organ not affected. Debris was removed infiltrating...
The retrograde suppression of the synaptic transmission by endocannabinoid sn-2-arachidonoylglycerol (2-AG) is mediated cannabinoid CB1 receptors and requires elevation intracellular Ca2+ activation specific 2-AG synthesizing (i.e. DAGLα) enzymes. However, anatomical organization neuronal substrates that express 2-AG/CB1 signaling system-related molecules associated with selective Ca2+-binding proteins (CaBPs) still unknown. For this purpose, we used double-label immunofluorescence confocal...
Msx1 is a regulatory gene involved in epithelio-mesenchymal interactions limb formation and organogenesis. In the embryonic CNS, expressed along dorsal midline. mutant mice have been obtained by insertion of nlacZ homeodomain. The most important features homozygous mutants that we observed were absence or malformation posterior commissure (PC) subcommissural organ (SCO), collapse cerebral aqueduct, development hydrocephalus. Heterozygous developed abnormal PC reduced SCO, as revealed...
In the present paper we describe facts that took place in rat brain after a single injection of enzyme neuraminidase from Clostridium perfringens into right lateral ventricle. After injection, it diffused through cerebrospinal fluid ipsilateral ventricle and third ventricle, about 400 µm periventricular parenchyma. The expression ICAM1 endothelial cells vessels, IBA1 microglia, GFAP astrocytes notably increased regions reached by injected neuraminidase. subependymal microglia ventricular...
Abstract Diet‐induced obesity produces changes in endocannabinoid signaling ( ECS ), influencing the regulation of energy homeostasis. Recently, we demonstrated that, high‐fat‐diet‐fed rats, blockade CB 1 receptor by AM 251 not only reduced body weight but also increased adult neurogenesis hippocampus, suggesting an influence diet on hippocampal cannabinoid function. To further explore role high‐fat‐diet‐induced obesity, investigated whether immunohistochemical expression enzymes that...