A5062683632- Neurogenesis and neuroplasticity mechanisms
- Cerebrospinal fluid and hydrocephalus
- Neuroinflammation and Neurodegeneration Mechanisms
- Neurobiology and Insect Physiology Research
- Neuroscience and Neuropharmacology Research
- Physiological and biochemical adaptations
- Aquaculture Nutrition and Growth
- Wnt/β-catenin signaling in development and cancer
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Erythrocyte Function and Pathophysiology
- Neuropeptides and Animal Physiology
- Zebrafish Biomedical Research Applications
- Neuroscience of respiration and sleep
- Neuroendocrine regulation and behavior
- Axon Guidance and Neuronal Signaling
- Regulation of Appetite and Obesity
- Lipid Membrane Structure and Behavior
- Cannabis and Cannabinoid Research
- Developmental Biology and Gene Regulation
- Hedgehog Signaling Pathway Studies
- Sleep and Wakefulness Research
- Cellular transport and secretion
- Fetal and Pediatric Neurological Disorders
- Neonatal and fetal brain pathology
- Circadian rhythm and melatonin
Universidad de Málaga
2009-2019
Instituto de Investigación Biomédica de Málaga
2015-2019
Pediatrics and Genetics
2014
It is known that microglia morphology and function are closely related, but only few studies have objectively described different morphological subtypes. To address this issue, parameters of microglial cells were analyzed in a rat model aseptic neuroinflammation. After the injection single dose enzyme neuraminidase (NA) within lateral ventricle (LV) an acute inflammatory process occurs. Sections from NA-injected animals sham controls immunolabeled with marker IBA1, which highlights...
Abstract In the brain of adult rats neurogenesis persists in subventricular zone lateral ventricles and dentate gyrus hippocampus. By contrast, low proliferative activity was observed hypothalamus. We report here that, after intracerebroventricular treatment with insulin‐like growth factor I (IGF‐I), cell proliferation significantly increased both periventricular parenchymal zones whole Neurons, astrocytes, tanycytes, microglia endothelial cells local vessels were stained marker...
Microglia are the resident macrophages in brain. Traditionally, two forms of microglia have been described: one considered as a resting/surveillant state which cells highly branched morphology, and another an activated they acquire de-ramified or amoeboid form. However, many studies describe intermediate microglial morphologies emerge during pathological processes. Since form function closely related, it is interest to correlate morphology with extent its activation. To address this issue,...
Lysophosphatidic acid (LPA) is a simple phospholipid with extracellular signaling properties mediated by specific G protein–coupled receptors. At least 2 LPA receptors, LPA1 and LPA2, are expressed in the developing brain, former enriched neurogenic ventricular zone (VZ), suggesting normal role neurogenesis. Despite numerous studies reporting effects of exogenous using vitro neural models, first loss-of-function mutants reported did not show gross cerebral cortical defects 50% that survived...
The lack of safe and effective treatments for obesity has increased interest in natural products that may serve as alternative therapies. From this perspective, we have analysed the effects daidzein, one main soy isoflavones, on diet-induced rats.Rats made obese after exposure to a very (60%) high fat-content diet were treated with daidzein (50 mg·kg(-1)) 14 days. dose was selected basis acute isoflavone feeding test. After days, animals killed plasma, white brown adipose tissue, muscle...
Hydrocephalic hyh mice are born with moderate hydrocephalus and a normal cerebral aqueduct. At about the fifth postnatal day aqueduct becomes obliterated severe develops. The aim of present investigation was to investigate mechanism this hydrocephalus, probably starting during fetal life when is still patent. By use immunocytochemistry scanning electron microscopy, mutant (n = 54) 61) mouse embryos were studied at various developmental stages trace earliest microscopic changes occurring in...
The ECS (endocannabinoid system) plays an important role in the onset of obesity and metabolic disorders, implicating central peripheral mechanisms predominantly via CB1 (cannabinoid type 1) receptors. receptor antagonist/inverse agonist treatment improves cardiometabolic risk factors insulin resistance. However, relative contribution organs to net beneficial effects remains unclear. In present study, we have identified presence endocannabinoid signalling machinery skeletal muscle also...
Soy extracts have been claimed to be neuroprotective against brain insults, an effect related the estrogenic properties of isoflavones. However, effects individual isoflavones on obesity-induced disruption adult neurogenesis not yet analyzed. In present study we explore pharmacological administration daidzein, a main soy isoflavone, in cell proliferation, apoptosis and gliosis hippocampus animals exposed very high-fat diet. Rats made obese after 12-week exposure standard or (HFD, 60%) diets...
Neuraminidase (NA) is a sialidase present, among various locations, in the envelope/membrane of some bacteria/viruses (e.g., influenza virus), and involved infectiveness and/or dispersion. The administration NA within brain lateral ventricle represents model acute sterile inflammation. relevance Toll-like receptors TLR2 TLR4 (particularly those microglial cells) such process was investigated.Mouse strains deficient either (TLR2-/-) or (TLR4-/-) were used. injected ventricle, inflammatory...
Endocannabinoids (ECs) are important neuromodulators involved in a plethora of physiological processes such as modulation synaptic transmission, neuroprotection, immune function, and neurodevelopment, among others. However, still lacking is detailed study on the presence this system circumventricular areas, brain structures controlling interaction between cerebrospinal fluid parenchyma. The aim work was to provide anatomical basis supporting functional role ECs activity areas. To end, an...
Endocannabinoid signalling participates in the control of neurogenesis, especially after brain insults. Obesity may explain alterations physiology affecting although it is unclear whether cannabinoid modulate neural proliferation obese animals. Here we analyse impact obesity by using two approaches, a high-fat diet (HFD, 60% fat) and standard/low-fat (STD, 10% fat), response to subchronic treatment with receptor type 1 (CB1) inverse agonist AM251 (3 mg/kg) on cell relevant neurogenic...
The subcommissural organ is an ependymal gland located at the entrance of cerebral aqueduct. It secretes glycoproteins into cerebrospinal fluid, where they aggregate to form Reissner's fiber. This fiber grows along aqueduct, fourth ventricle, and central canal. There evidence that involved in pathogenesis congenital hydrocephalus. was investigated mutant mouse hyh developing a nervous system normal hydrocephalic mice, 1 40 days old, using antibodies recognizing secretory glycoproteins, by...
The subventricular zone of the striatal wall adult rodents is an active neurogenic region for life. Cubic multiciliated ependyma separates from cerebrospinal fluid (CSF) and involved in control neurogenesis. By injecting neuraminidase Clostridium perfringens into right lateral ventricle rat, we provoked a partial detachment wall. contralateral was never affected used as experimental control. Neuraminidase caused widening intercellular spaces among some ependymal cells their subsequent...
Cannabinoid CB1 receptors peripherally modulate energy metabolism. Here, we investigated the role of in expression glucose/pyruvate/tricarboxylic acid (TCA) metabolism rat abdominal muscle. Dihydrolipoamide dehydrogenase (DLD), a flavoprotein component (E3) α-ketoacid complexes with diaphorase activity mitochondria, was specifically analyzed. After assessing effectiveness receptor antagonist AM251 (3 mg kg-1, 14 days) on food intake and body weight, could identified seven key enzymes from...
To investigate the role of sialic acid in ependyma rat brain, we injected neuraminidase from Clostridium perfingens into lateral ventricle 86 adult rats that were sacrificed at various time intervals. After administration 10 μg neuraminidase, ciliated cuboidal ependymal cells ventricles, third ventricle, cerebral aqueduct, and rostral half fourth died detached. The regions sealed by tight juntions such as choroid plexus subcommissural organ not affected. Debris was removed infiltrating...