A5041566578- Neuroscience and Neuropharmacology Research
- Cannabis and Cannabinoid Research
- Neurotransmitter Receptor Influence on Behavior
- Sleep and Wakefulness Research
- Ion channel regulation and function
- Hearing, Cochlea, Tinnitus, Genetics
- Biochemical Analysis and Sensing Techniques
- Olfactory and Sensory Function Studies
- Neural dynamics and brain function
- Neuroinflammation and Neurodegeneration Mechanisms
- Pain Mechanisms and Treatments
- Pancreatic function and diabetes
- Biochemical effects in animals
- Photoreceptor and optogenetics research
- Genetic Neurodegenerative Diseases
- Neuroscience of respiration and sleep
- Memory and Neural Mechanisms
- Ion Channels and Receptors
- Forensic Toxicology and Drug Analysis
- Tryptophan and brain disorders
- Pharmacology and Obesity Treatment
- Fatty Acid Research and Health
- Prenatal Substance Exposure Effects
- Multiple Sclerosis Research Studies
- Apelin-related biomedical research
University of the Basque Country
2016-2025
Achucarro Basque Center for Neuroscience
2015-2024
Euskadiko Parke Teknologikoa
2015-2017
Hospital Universitario de La Princesa
2013
Inserm
2011
Neurocentre Magendie
2008-2011
Université de Bordeaux
2008
University of Oslo
2005
Similar to dopamine (DA), cannabinoids strongly influence prefrontal cortical functions, such as working memory, emotional learning, and sensory perception. Although endogenous cannabinoid receptors (CB 1 Rs) are abundantly expressed in the cortex (PFC), very little is known about endocannabinoid (eCB) signaling this brain region. Recent behavioral electrophysiological evidence has suggested a functional interplay between receptor systems, although cellular mechanisms underlying interaction...
Brain mitochondrial activity is centrally involved in the central control of energy balance. When studying functions brain, however, discrepant results might be obtained, depending on experimental approaches. For instance, immunostaining experiments and biochemical isolation organelles expose investigators to risks false positive and/or negative results. As an example, functional presence cannabinoid type 1 (CB1) receptors brain membranes (mtCB1) was recently reported rapidly challenged,...
The cannabinoid type 1 (CB1) receptor is widely distributed in the brain and peripheral organs where it regulates cellular functions metabolism. In brain, CB1 mainly localized on presynaptic axon terminals but also found mitochondria (mtCB1), respiration energy production. Likewise, muscle mitochondria, very little known about it. aim of this study was to further investigate detail distribution functional role mtCB1 three different striated muscles. Immunoelectron microscopy for used...
Abstract Astroglial type‐1 cannabinoid (CB 1 ) receptors are involved in synaptic transmission, plasticity and behavior by interfering with the so‐called tripartite synapse formed pre‐ post‐synaptic neuronal elements surrounding astrocyte processes. However, little is known concerning subcellular distribution of astroglial CB receptors. In particular, brain mostly localized at cells' plasmalemma, but recent evidence indicates their functional presence mitochondrial membranes. Whether present...
We investigated the mechanisms by which activation of group I metabotropic glutamate receptors (mGluRs) and CB1 cannabinoid (CB1Rs) leads to inhibition synaptic currents at calyx Held synapse in medial nucleus trapezoid body (MNTB) rat auditory brainstem. In ∼50% MNTB neurons tested, mGluRs specific agonist (s)-3,5-dihydroxyphenylglycine (DHPG) reversibly inhibited AMPA receptor- NMDA receptor-mediated EPSCs a similar extent reduced paired-pulse depression, suggestive an release. Presynaptic...
We examined the effect of glutamate transporter blockade at calyx Held synapse. In immature synapses [defined as postnatal day 8 (P8) to P10 rats], causes tonic activation NMDA receptors and strong inhibition AMPA receptor-mediated EPSC amplitude. was blocked with a metabotropic receptor (mGluR) antagonist [1 microm LY341495 (2S-2-amino-2-(1S,2S-2-carboxycycloprop-1-yl)-3-(xanth-9-yl)propanoic acid)], suggesting that elevated resting concentration specifically activates group II III mGluRs....
Alterations of long-term synaptic plasticity have been proposed to participate in the development addiction. To preserve functions, homeostatic processes must be engaged after exposure abused drugs. At mouse cortico-accumbens synapses, a single vivo injection Δ 9 -tetrahydrocannabinol (THC) suppresses endocannabinoid-mediated depression. Using biochemical and electrophysiological approaches, we now report that 1 week repeated THC treatment reduces coupling efficiency cannabinoid CB1...
The endocannabinoid system is involved in multiple physiological functions including reward. Cannabinoids potently control the activity of midbrain dopamine cells, but contribution cortical projections this phenomenon unclear. We show that bed nucleus stria terminalis (BNST) efficient relays excitation to neurons ventral tegmental area (VTA). Anatomical and vivo electrophysiological evidence demonstrate excitatory arising exclusively from infralimbic cortex converge on BNST neurons, which...
The dorsal striatum is a key node for many neurobiological processes such as motor activity, cognitive functions, and affective processes. proper functioning of striatal neurons relies critically on metabotropic receptors. Specifically, the main adenosine endocannabinoid receptors present in striatum, ie, A2A receptor (A2AR) cannabinoid CB1 (CB1R), are pivotal importance control neuronal excitability. Facilitatory inhibitory functional interactions between A2AR CB1R have been reported,...
The failure to undergo remyelination is a critical impediment recovery in multiple sclerosis. Chondroitin sulfate proteoglycans (CSPGs) accumulate at demyelinating lesions creating nonpermissive environment that impairs axon regeneration and remyelination. Here, we reveal new role for 2-arachidonoylglycerol (2-AG), the major CNS endocannabinoid, modulation of CSPGs deposition progressive model sclerosis, Theiler9s murine encephalomyelitis virus-induced disease. Treatment with potent...
Background The bed nucleus of the stria terminalis (BNST) is involved in behaviors related to natural reward, drug addiction and stress. In spite emerging role endogenous cannabinoid (eCB) system these behaviors, little known about anatomy function this anterolateral BNST (alBNST). aim study was provide a detailed morphological characterization localization 1 (CB1) receptor necessary step toward better understanding physiological roles eCB region brain. Methodology/Principal Findings We have...
ABSTRACT Type 1 cannabinoid (CB ) receptors are widely distributed in the brain. Their physiological roles depend on their distribution pattern, which differs remarkably among cell types. Hence, subcellular compartments with little but functionally relevant CB can be overlooked, fostering an incomplete mapping. To overcome this, knockin mice cell‐type–specific rescue of have emerged as excellent tools for investigating receptors’ localization and sufficient functional role no bias. However,...
The endocannabinoid system modulates synaptic plasticity in the hippocampus, but a link between long-term and type 1 cannabinoid (CB1) receptor at medial perforant path (MPP) synapses remains elusive. Here, immuno-electron microscopy adult mice showed that ∼26% of excitatory terminals middle 1/3 dentate molecular layer (DML) contained CB1 receptors, field postsynaptic potentials evoked by MPP stimulation were inhibited activation. In addition, 10 Hz for min triggered receptor-dependent...
The nucleus accumbens (NAc) plays a key role in drug-related behavior and natural reward learning. Synaptic plasticity dopamine D1 D2 receptor medium spiny neurons (MSNs) of the NAc endogenous cannabinoid (eCB) system have been implicated seeking. However, precise molecular physiological basis reward-seeking remains unknown. We found that specific deletion metabotropic glutamate 5 (mGluR5) D1-expressing MSNs (D1miRmGluR5 mice) abolishes eCB-mediated long-term depression (LTD) prevents...
Emotions and behavior can be affected by social chemosignals from conspecifics. For instance, olfactory signals stressed individuals induce stress-like physiological synaptic changes in naïve partners. Direct stress also alters cognition, but the impact of socially transmitted on memory processes is currently unknown. Here we show that exposure to produced sufficient impair retrieval unstressed male mice. This requires astrocyte control information bulb mediated mitochondria-associated CB1...
Studies indicate that metabotropic glutamate receptors (mGluRs) may play a role in spinal sensory transmission. We examined the cellular and subcellular distribution of mGluR subtype 4a tissue by means specific antiserum immunocytochemical techniques for light electron microscopy. A dense plexus mGluR4a-immunoreactive elements was seen dorsal horn, with an apparent accumulation lamina II. The immunostaining composed sparse immunoreactive fibres punctate elements. No perikaryal staining seen....