A5088576349- Cancer, Hypoxia, and Metabolism
- Amino Acid Enzymes and Metabolism
- Cancer Research and Treatments
- RNA modifications and cancer
- Epigenetics and DNA Methylation
- Metabolism and Genetic Disorders
- Cancer-related molecular mechanisms research
- Digestive system and related health
- Cellular transport and secretion
- Neuroscience and Neuropharmacology Research
- Lysosomal Storage Disorders Research
- Mitochondrial Function and Pathology
- Biochemical and Molecular Research
- Plant biochemistry and biosynthesis
- Pancreatitis Pathology and Treatment
- Molecular Biology Techniques and Applications
- Advanced biosensing and bioanalysis techniques
- Virus-based gene therapy research
- Postharvest Quality and Shelf Life Management
- Plant Surface Properties and Treatments
- Peroxisome Proliferator-Activated Receptors
- Sphingolipid Metabolism and Signaling
- Click Chemistry and Applications
- ATP Synthase and ATPases Research
- Diet, Metabolism, and Disease
Universidad de Málaga
2012-2024
Instituto de Investigación Biomédica de Málaga
2015-2021
Background Glutaminase is expressed in most mammalian tissues and cancer cells, but the regulation of its expression poorly understood. An essential step to accomplish this goal characterization species- cell-specific isoenzyme pattern expression. Our aim was identify characterize transcript variants glutaminase Gls2 gene. Methodology/Principal Findings We demonstrate for first time simultaneous two from gene human, rat mouse. A combination RT-PCR, primer-extension analysis, bioinformatics,...
The expression of glutaminase in glial cells has been a controversial issue and matter debate for many years. Actually, is essentially considered as neuronal marker brain. Astrocytes are endowed with efficient high capacity transport systems to recapture synaptic glutamate which seems be consistent the absence these cells. In this work, comprehensive study was devised elucidate neuroglia and, more concretely, astrocytes. Immunocytochemistry rat human brain tissues employing isoform‐specific...
Glutaminase (GA) catalyzes the first step in mitochondrial glutaminolysis playing a key role cancer metabolic reprogramming. Humans express two types of GA isoforms: GLS and GLS2. isozymes have been consistently related to cell proliferation, but GLS2 remains poorly understood. is repressed many tumor cells better understanding its function tumorigenesis may further development new therapeutic approaches. We analyzed expression HCC, GBM neuroblastoma cells, as well monkey COS-7 cells....
Cannabinoid CB1 receptors peripherally modulate energy metabolism. Here, we investigated the role of in expression glucose/pyruvate/tricarboxylic acid (TCA) metabolism rat abdominal muscle. Dihydrolipoamide dehydrogenase (DLD), a flavoprotein component (E3) α-ketoacid complexes with diaphorase activity mitochondria, was specifically analyzed. After assessing effectiveness receptor antagonist AM251 (3 mg kg-1, 14 days) on food intake and body weight, could identified seven key enzymes from...
Abstract Glutamine behaves as a key nutrient for tumors and rapidly dividing cells. Glutaminase is the main glutamine‐utilizing enzyme in these cells, its activity correlates with glutamine consumption growth rate. We have carried out antisense L‐type glutaminase inhibition human MCF7 breast cancer order to study effect on hexosamine pathway pattern of protein O‐glycosylation. The mRNA expressing named ORF19, presented 50% lower proliferation rate than parental showing more differentiated...
Abstract Background Glutaminase isoenzymes GLS and GLS2 play apparently opposing roles in cancer: acts as an oncoprotein, while (GAB isoform) has context specific tumour suppressive activity. Some microRNAs (miRNAs) have been implicated progression of tumours, including gliomas. The aim was to investigate the effect GAB expression on both miRNAs oxidative status glioblastoma cells. Methods Microarray profiling miRNA performed GLS-silenced LN229 GAB-transfected T98G human cells their...
Lysophosphatidic acid (LPA) is an extracellular lipid mediator that regulates nervous system development and functions acting through G protein-coupled receptors. Here we explore the crosstalk between LPA1 receptor glutamatergic transmission by examining expression of glutaminase isoforms in different brain areas isolated from wild-type KOLPA1 mice. Silencing induced a severe down-regulation KGA (GLS) protein several regions, particularly cortex hippocampus. Immunohistochemical assessment...
Abstract Glutaminase is considered as the main glutamate producer enzyme in brain. Consequently, essential for both glutamatergic and gabaergic transmissions. Glutamine-derived ammonia, products of glutaminase reaction, fulfill crucial roles energy metabolism biosynthesis basic metabolites, such GABA, proteins glutathione. However, ammonia are also hazardous compounds danger lurks their generation beyond normal physiological thresholds; hence, activity must be carefully regulated mammalian...
Iduronate-2-sulfatase (IDS) is a lysosomal enzyme involved in the metabolism of glycosaminoglycans heparan (HS) and dermatan (DS) sulfate. Mutations on IDS gene produce mucopolysaccharidosis II (MPS II), characterized by accumulation HS DS, leading to severe damage central nervous system (CNS) other tissues. In this study, we used neurochemistry proteomic approaches identify brain distribution its interacting proteins wild-type mouse brain. immunoreactivity showed robust staining throughout...
Ehrlich ascites tumor cells (EATC) is a highly proliferative malignant cell line derived from mouse mammary epithelia, whereas their derivative, 0.28AS-2 cells, expressing antisense glutaminase mRNA, show less transformed phenotype and loss of tumorigenic capacity in vivo correlated with an inhibition expression. The mRNA differential display technique was applied to these two lines for the identification isolation genes whose transcription altered. Side-by-side comparisons cDNA patterns...
Abstract Here we report the synthesis and characterization of several tripod‐shaped oligo( p ‐phenylene)s molecules with legs composed five three phenylene units, compounds 1 2 , respectively. In these structures, each leg is end‐capped an azide group terminal functional arm contains acetylene or bromine group. The presented has as key step a Pd‐catalyzed Suzuki cross‐coupling reaction. particular, iodine derivative from silicon core molecule reacts appropriate ‐phenylene) boron derivative,...
The Lysosomal Storage disease known as Mucopolysaccharidosis type II, is caused by mutations affecting the iduronate-2-sulfatase required for heparan and dermatan sulfate catabolism. central nervous system (CNS) mostly severely affected accumulation of both substrates. complexity CNS damage observed in MPS II patients has been limitedly explored. use mass spectrometry (MS)-based proteomics tools to identify protein profiles may yield valuable information about pathological mechanisms Hunter...
An amendment to this paper has been published and can be accessed via a link at the top of paper.