A5072034767- Extracellular vesicles in disease
- Cancer Genomics and Diagnostics
- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- Inflammatory Biomarkers in Disease Prognosis
- Radiomics and Machine Learning in Medical Imaging
- Glioma Diagnosis and Treatment
- Pulmonary Hypertension Research and Treatments
- Lung Cancer Treatments and Mutations
- Ferroptosis and cancer prognosis
- Platelet Disorders and Treatments
- Sarcoma Diagnosis and Treatment
- Pancreatic and Hepatic Oncology Research
- Brain Metastases and Treatment
- Lung Cancer Research Studies
- Ovarian cancer diagnosis and treatment
- Microtubule and mitosis dynamics
- Multiple Myeloma Research and Treatments
- Lung Cancer Diagnosis and Treatment
- Cancer, Lipids, and Metabolism
- DNA Repair Mechanisms
- Blood properties and coagulation
- Molecular Biology Techniques and Applications
- Ubiquitin and proteasome pathways
- Cancer Immunotherapy and Biomarkers
The Netherlands Cancer Institute
2013-2025
Cancer Center Amsterdam
2015-2023
Amsterdam University Medical Centers
2020-2023
Vrije Universiteit Amsterdam
2021-2023
Amsterdam UMC Location Vrije Universiteit Amsterdam
2013-2022
Amsterdam Neuroscience
2020-2022
University of Amsterdam
2021
University of Würzburg
2013
Harvard University
2013
Dana-Farber Cancer Institute
2013
Blood-based liquid biopsies, including tumor-educated blood platelets (TEPs), have emerged as promising biomarker sources for non-invasive detection of cancer. Here we demonstrate that particle-swarm optimization (PSO)-enhanced algorithms enable efficient selection RNA panels from platelet RNA-sequencing libraries (n = 779). This resulted in accurate TEP-based early- and late-stage non-small-cell lung cancer 518 validation cohort, accuracy, 88%; AUC, 0.94; 95% CI, 0.92–0.96; p < 0.001; n 106...
BackgroundGlioblastomas exhibit a high level of chemotherapeutic resistance, including to the antimitotic agents vincristine and taxol. During mitotic agent-induced arrest, glioblastoma cells are able perform damage-control self-repair continue proliferation. Monopolar spindle 1 (MPS1/TTK) is checkpoint kinase gatekeeper arrest.
Tumor-educated platelets (TEPs) are potential biomarkers for cancer diagnostics. We employ TEP-derived RNA panels, determined by swarm intelligence, to detect and monitor glioblastoma. assessed specificity comparing the spliced profile of TEPs from glioblastoma patients with multiple sclerosis brain metastasis (validation series, n = 157; accuracy, 80%; AUC, 0.81 [95% CI, 0.74–0.89; p < 0.001]). Second, analysis versus asymptomatic healthy controls in an independent validation series (n 347)...
Diffuse gliomas are the most common malignant primary tumors of central nervous system. Like other neoplasms, these release molecular information into circulation. Tumor-derived biomarkers include proteins, nucleic acids, and tumor-derived extracellular vesicles that accumulate in plasma, serum, blood platelets, urine and/or cerebrospinal fluid. Recently, also circulating tumor cells have been identified glioma patients. Circulating molecules, vesicles, may be useful as easily accessible...
Sarcoma is a heterogeneous group of rare malignancies arising from mesenchymal tissues. Recurrence rates are high and methods for early detection by blood-based biomarkers do not exist. Hence, development liquid biopsies as disease recurrence monitoring would be an important step forward. Recently, it has been shown that tumor-educated platelets (TEPs) harbor specific spliced ribonucleic acid(RNA)-profiles. These RNA-repertoires potentially applicable cancer diagnostics. We aim to evaluate...
Tumor-educated Platelets (TEPs) have emerged as rich biosources of cancer-related RNA profiles in liquid biopsies applicable for cancer detection. Although human blood platelets been found to be enriched circular (circRNA), no studies investigated the potential circRNA platelet-derived biomarkers cancer. In this proof-of-concept study, we examine whether signature can used a biopsy biomarker detection non-small cell lung (NSCLC). We analyzed total RNA, extracted from platelet samples...
Abstract Platelets are reprogrammed by cancer via a process called education, which favors development. The transcriptional profile of tumor-educated platelets (TEPs) is skewed and therefore practicable for detection. This intercontinental, hospital-based, diagnostic study included 761 treatment-naïve inpatients with histologically confirmed adnexal masses 167 healthy controls from nine medical centers (China, n = 3; Netherlands, 5; Poland, 1) between September 2016 May 2019. main outcomes...
Liquid biopsy approaches offer a promising technology for early and minimally invasive cancer detection. Tumor-educated platelets (TEPs) have emerged as liquid biosource the detection of various types. In this study, we processed analyzed TEPs collected from 466 Non-small Cell Lung Carcinoma (NSCLC) patients 410 asymptomatic individuals (controls) using previously established thromboSeq protocol. We developed novel particle-swarm optimization machine learning algorithm which enabled...
Extracellular vesicles (EVs) are small nanometre-sized that circulating in blood. They released by multiple cells, including tumour cells. We hypothesized EVs contain protein kinases may be assessed as biomarkers during treatment with tyrosine kinase inhibitors.EVs U87 glioma H3255 and H1650 non-small-cell lung cancer (NSCLC) cells were profiled tandem mass spectrometry. Total AKT/protein B extracellular signal regulated 1/2 (ERK1/2) levels well their relative phosphorylation measured...
Background and objectives: Serum neurofilament light (sNfL) is a biomarker for neuro-axonal damage in multiple sclerosis (MS). Clinical implementation remains limited. We investigated the impact of on clinical decisions using questionnaires at MS Center Amsterdam, tertiary outpatient clinic. Methods: sNfL assessments were added to routine practice (August 2021–December 2022). Before after results, clinicians filled context testing, decisions, certainty herein, expectation magnetic resonance...
Background In multiple sclerosis (MS), clinical assessment, MRI and cerebrospinal fluid are important in the diagnostic process. However, no blood biomarker has been confirmed as a useful tool work-up. Objectives Blood platelets contain rich spliced mRNA repertoire that can alter during megakaryocyte development but also platelet formation circulation. this proof of concept study, we evaluate potential RNA for detection MS. Methods We isolated sequenced samples obtained from 57 MS patients...
Diagnosis and treatment of leptomeningeal metastases (LM) in epidermal growth factor receptor mutation-positive (EGFRm +) non-small-cell lung carcinoma (NSCLC) is challenging. We aimed to identify resistance mechanisms (RM) osimertinib cerebrospinal fluid (CSF) plasma.
Background: Breast cancer stage at diagnosis has a large impact on the 10-year survival probability. Mammography based screening enabled early detection of breast cancer. However, mammography limited sensitivity, and suffers from overdiagnosis overtreatment. We aim to improve by developing classification algorithm mRNA signatures blood platelets (Tumor Educated Platelets; TEP). Methods: Platelet was sequenced 265 women with I-IV cancer, 216 asymptomatic female controls, 144 non-cancerous...
Rationale: Pulmonary hypertension encompasses progressive disorders leading to right ventricular dysfunction and early death. Late detection is an important cause of poor clinical outcomes. However, biomarkers that accurately predict the presence pulmonary are currently lacking. Objectives: In this study, we provide evidence blood platelets contain a distinctive ribonucleic acid (RNA) profile may be exploited for hypertension. Methods: Blood platelet RNA was isolated prospectively from 177...
Anti-PD-(L)1 immunotherapy has emerged as a promising treatment approach for non-small cell lung cancer (NSCLC), though the response rates remain low. Pre-treatment prediction may improve patient allocation immunotherapy. Blood platelets act active immune-like cells, thereby constraining T-cell activity, propagating metastasis, and adjusting their spliced mRNA content. We investigated whether platelet RNA profiles before start of nivolumab anti-PD1 predict responses. performed RNA-sequencing...
Abstract Pancreatic ductal adenocarcinoma (PDAC) is often diagnosed at advanced disease stages and invasive procedures are needed to confirm diagnosis. Innovative liquid biopsy-based approaches warranted diagnose early prevent unnecessary treatment for or misdiagnosed benign diseases. CA19.9 the only clinically implemented biomarker, but its sensitivity inadequate it lacks specificity. Spliced messenger RNA (mRNA) of tumor-educated platelets (TEPs) emerged recently as new potent pan-cancer...
INTRODUCTION: Blood-based liquid biopsies are a potential approach for leveraging clinical cancer diagnostics and patients' treatment. However, so far, diagnostic, prognostic, predictive, monitoring biomarkers patients with diffuse glioma only limitedly available, require further validation. Tumor-educated blood platelets (TEPs) sequester several biomolecules, including EGFRvIII mRNA, during tumor growth, thereby altering their RNA profile. Therefore, we reasoned that TEPs may serve as an...