A5009256892- Drug Transport and Resistance Mechanisms
- Cholesterol and Lipid Metabolism
- Blood disorders and treatments
- Immunodeficiency and Autoimmune Disorders
- Metabolism and Genetic Disorders
- Diabetes and associated disorders
- RNA and protein synthesis mechanisms
- Neurogenetic and Muscular Disorders Research
- Inflammatory Myopathies and Dermatomyositis
- Adrenal Hormones and Disorders
- Pharmacological Effects and Toxicity Studies
- Biomedical Research and Pathophysiology
- Systemic Sclerosis and Related Diseases
- Autoimmune and Inflammatory Disorders
- Lipid Membrane Structure and Behavior
- Cardiac Arrhythmias and Treatments
- Genetic Neurodegenerative Diseases
- Eosinophilic Disorders and Syndromes
- Biochemical and Molecular Research
- Soft tissue tumor case studies
- Glycogen Storage Diseases and Myoclonus
- Neurological disorders and treatments
- Dermatological and Skeletal Disorders
- Protein Tyrosine Phosphatases
- Streptococcal Infections and Treatments
University of Ottawa
2018-2025
Bipar
2021
The University of Texas Southwestern Medical Center
2016-2017
Southwestern Medical Center
2017
Texas Tech University
2007-2010
Texas Tech University Health Sciences Center
2007-2010
Texas A&M University System
2009
University of California, Riverside
2002-2007
University of Rochester Medical Center
2007
High-density lipoprotein (HDL) particles form during cellular cholesterol removal, positioning HDL biogenesis as a potential strategy to combat atherosclerosis. We identified desmocollin 1 (DSC1) negative regulator of and discovered that docetaxel (DTX) effectively inhibits DSC1 activity. This study assessed the efficacy DTX in reducing atherosclerosis ApoE−/− mice. After two weeks on high-fat diet, mice were divided into baseline, vehicle-treated, DTX-treated groups. Baseline sacrificed at...
The structure of P-glycoprotein (Pgp) from mouse has been studied by electron microscopy and image analysis. Two-dimensional crystals Pgp in a lipid bilayer were generated reconstituting pure, detergent-solubilized protein containing C-terminal six-histidine tag using the monolayer technique. belong to plane group P1 with = b 104 ± 2 Å γ 90 4°. projection calculated at resolution 22 shows two closely interacting domains that can be interpreted as N- halves protein. is consistent recently...
P-glycoprotein (Pgp) is an ATP hydrolysis driven multidrug efflux pump, which, when overexpressed in the plasma membrane of certain cancers, can lead to failure chemotherapy. Previously, we have presented a projection structure nucleotide-free mouse Pgp from electron microscopic images lipid monolayer-generated two-dimensional crystals ( Lee, J. Y., Urbatsch, I. L., Senior, A. E., and Wilkens, S. (2002) Biol. Chem. 277, 40125-40131 ). Here analyzed cysteine-free human that were generated...
ABCG5 and ABCG8 are half-size ABC transporters that function as heterodimers (ABCG5/G8) to reduce sterol absorption in the intestines increase excretion from liver. Previous studies demonstrated bile acids increased ABCG5/G8 specific cholesterol efflux cell models. In this study we tested effects of on ATP hydrolysis Pichia pastoris purified found they stimulated approximately 20-fold wild-type but not a hydrolysis-deficient mutant. Nonconjugated cholate supported highest ATPase activity...
Human ATP-binding cassette (ABC) transporters comprise a family of 48 membrane-spanning transport proteins, many which are associated with genetic diseases or multidrug resistance cancers. In this study, we present comprehensive approach for the cloning, expression, and purification human ABC in yeast Pichia pastoris. We analyzed expression 25 proteins demonstrate that 11 transporters, including ABCC3, ABCB6, ABCD1, ABCG1, ABCG4, ABCG5, ABCG8, ABCE1, ABCF1, ABCF2, ABCF3, were expressed at...
The heterodimeric ATP-binding cassette (ABC) sterol transporter, ABCG5/G8, is responsible for the biliary and transintestinal secretion of cholesterol dietary plant sterols. Missense mutations ABCG5/G8 can cause sitosterolemia, a loss-of-function disorder characterized by accumulation premature atherosclerosis. A new molecular framework was recently established crystal structure human reveals network polar charged amino acids in core transmembrane domains, namely, relay. In this study, we...
Lipid composition in the cellular membranes plays an important role maintaining struc-tural integrity of cells and regulating signaling that controls functions both mem-brane-anchored cytoplasmic proteins. ATP-dependent ABC P4-ATPase lipid transport-ers, two integral membrane proteins, are known to contribute translocation across li-pid bilayers on membranes. In this review, we will highlight current knowledge about cholesterol phospholipids cell how transporters participate process.