A5078275124- Antimicrobial Resistance in Staphylococcus
- Microbial Metabolism and Applications
- Probiotics and Fermented Foods
- Plant-Microbe Interactions and Immunity
- Plant Pathogenic Bacteria Studies
- Bacterial Identification and Susceptibility Testing
- Bacterial biofilms and quorum sensing
- Legume Nitrogen Fixing Symbiosis
- Microbial infections and disease research
- Identification and Quantification in Food
- Genomics and Phylogenetic Studies
- Salmonella and Campylobacter epidemiology
- Escherichia coli research studies
- Respiratory viral infections research
- Bacterial Genetics and Biotechnology
- Biosensors and Analytical Detection
- Rabies epidemiology and control
- Animal Virus Infections Studies
- Phytoplasmas and Hemiptera pathogens
- Bacillus and Francisella bacterial research
- Plant pathogens and resistance mechanisms
- Mycotoxins in Agriculture and Food
- Antibiotic Resistance in Bacteria
- Clostridium difficile and Clostridium perfringens research
- Bacteriophages and microbial interactions
Agence Nationale de Sécurité Sanitaire de l’Alimentation, de l’Environnement et du Travail
2018-2025
Paris-Est Sup
2023-2024
Université d'Angers
2016-2017
Institut de Recherche en Horticulture et Semences
2016-2017
Institut Agro Rennes-Angers
2016-2017
Deciphering the evolutionary history and transmission patterns of virulence determinants is necessary to understand emergence novel pathogens. The main determinant most pathogenic proteobacteria type three secretion system (T3SS). Xanthomonas genus includes bacteria responsible for numerous epidemics in agroecosystems worldwide represents a major threat plant health. factor Hrp2 family T3SS; however, this not conserved all strains it has been previously determined whether distribution T3SS...
Food contamination by staphylococcal enterotoxins (SEs) is responsible for many food poisoning outbreaks (FPOs) each year, and they represent the third leading cause of FPOs in Europe. SEs are a protein family composed 27 SEs. However, enzyme immunoassays can detect only five classical (SEA-SEE) directly food. Thus, molecular characterization methods strains found now used FPO investigations. Here, we describe on development implementation genomic analysis tool called NAuRA (Nice automatic...
Staphylococcal enterotoxins (SEs) are responsible for frequent food poisoning outbreaks worldwide. Specific identification of SEs is crucial confirmation poisoning, tracking the incriminated foods or ingredients, and removal from chain. Here, we report on a new testing protocol addressing challenge low abundance in contaminated high sequence heterogeneity. Multiplex ability targeted high-resolution mass spectrometry was succesfully applied to simultaneous quantitative determination eight...
The acquisition of virulence-related genes through horizontal gene transfer can modify the pathogenic profiles strains and lead to emergence new diseases. Xanthomonas arboricola is a bacterial species largely known for damage it causes stone nut fruit trees worldwide. In addition these host-specific populations called pathovars, many nonpathogenic have been identified in this species. Their evolutionary significance context pathogen unknown. We looked at seven housekeeping amplified from 187...
Currently, only 5 (SEA to SEE) out of 27 known staphylococcal enterotoxins can be analyzed using commercially available kits. Six genes (seg, sei, sem, sen, seo, and seu), encoding putative undetectable enterotoxins, are located on the enterotoxin gene cluster (egc), which is part Staphylococcus aureus genomic island vSaβ. These have been described as likely being involved in food-poisoning outbreaks. The aim present study was determine if whole-genome data used for prediction egc...
Staphylococcus aureus is a major human pathogen and an important cause of livestock infections. More than 20 staphylococcal enterotoxins with emetic activity can be produced by specific strains responsible for food poisoning, one the most common food-borne diseases. Whole genome sequencing provides comprehensive view structure gene content that have largely been applied in outbreak investigations genomic comparisons. In this study, six enterotoxigenic S. were characterised using combination...
In the context of pathogen surveillance, it is crucial to ensure interoperability and harmonized data. Several surveillance systems are designed compare bacteria identify outbreak clusters based on core genome MultiLocus Sequence Typing (cgMLST). Among different approaches available generate bacterial cgMLST, our research used an assembly-based approach (chewBBACA tool).
We addressed here the need for improved sensitivity of top-down mass spectrometry identification, differentiation, and absolute quantification sequence variants SEA, a bacterial toxin produced by Staphylococcus aureus regularly involved in food poisoning outbreaks (FPO). combined immunoaffinity enrichment, protein internal standard, optimized acquisition conditions, either full-scan high-resolution (HRMS) or multiplex parallel reaction monitoring (PRM) mode. Deconvolution HRMS signal PRM...
<title>Abstract</title> Background In the context of pathogen surveillance, it is crucial to ensure interoperability and harmonized data. Several surveillance systems are designed compare bacteria identify outbreak clusters based on core genome MultiLocus Sequence Typing (cgMLST). Among different approaches available generate bacterial cgMLST, our research used an assembly-based approach (chewBBACA tool). Methods Simulations short-read sequencing were conducted for 5 genomes 27 pathogens...